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Thermo Electron C3i, User Manual

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Thermo Electron C3i User Manual Download Page 2

CAREFULLY READ THIS MANUAL BEFORE OPERATING YOUR INSTRUMENT.

INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF

THERMO ELECTRON CORPORATION;

IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT THE OWNER’S AUTHORIZATION.

THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE INSTRUCTIONS

AND PRECAUTIONS DESCRIBED IN THIS DOCUMENT

C3i-CR3i 

Summary of Contents for C3i

Page 1: ...C3i CR3i Jouan 4 x 280 mL Multifunction Centrifuges User s Manual Manual N 89003152 a ...

Page 2: ...TAINED IN THIS DOCUMENT IS THE PROPERTY OF THERMO ELECTRON CORPORATION IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT THE OWNER S AUTHORIZATION THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS DOCUMENT C3i CR3i ...

Page 3: ...Revision Status C3i CR3i Revision Status REV DATE AMENDED PAGES NOTES a 07 04 Initial release ...

Page 4: ...ocking wrench 1 c 86003941 Fuse 5X20 3 15A T 250V 2 c Item number Quantity Check Centrifuge 1 c User manual 1 c CE Declaration of compliance 1 c Manual lid opening instructions 1 c Mains cord 1 c Toolbox Rotor removal tool 1 c Emergency unlocking tool 1 c Spare fuses C3i only 2 c c 11175770 Centrifuge C3i 230V 50 60 Hz c 11175771 Centrifuge C3i 120V 60 Hz c 11175774 Centrifuge CR3i 230V 50 60 Hz c...

Page 5: ...5 48 16 62 97 Finland Vantaa Phone 358 9 329 100 Fax 358 9 3291 0414 France Saint Herblain Nantes Phone 33 0 2 28 03 20 00 Fax 33 0 2 28 03 20 01 Germany Dreieich Phone 49 6103 408 0 Fax 49 6103 408 1222 Hong Kong Wanchai Phone 852 2885 4613 Fax 852 2567 4447 India Navi Mumbai Phone 91 22 2778 1101 Fax 91 22 2778 1103 Italy Cologno Monzese MI Phone 39 02 253 90889 Fax 39 02 253 8922 Japan Yokohama...

Page 6: ...Use 13 4 1 Controls and Indicators 13 4 2 Display Screens 14 4 3 Control Panel Functions 14 4 4 Control Panel Indicators 15 4 5 Description of Certain Events with Respect to the Control System 16 4 6 Preparing the First Run of the Day 16 4 7 Sample Loading 17 5 Hazards Precautions and Limitations of Use 19 5 1 IEC 1010 2 020 19 5 2 Cautions 19 5 3 Speed Control 19 5 4 Operational Limitations 20 5 ...

Page 7: ... unobserved could result in damage to the appliance and or harm to the operator In this manual this symbol means that you will find important information about minimizing biological risk if unobserved the result could be harm to the operator 1 3 Description The C3i and CR3i centrifuges are designed for laboratory use They will separate the components of fluids into layers of varying density by sub...

Page 8: ... to be drained from the bowl The drain hose exits at the back of the machine Near the drain hose you can find the following label To remove condensation please follow this procedure Switch off the centrifuge and disconnect the power Put a small basin under the hose Unplug the hose Drain the centrifuge bowl Insert the plug back into the hose ATTENTION Do not remove the plug from the drain hose duri...

Page 9: ...o balance the load call your Thermo Service representative Note Imbalance tolerance depends upon the rotor in use The centrifuge will tolerate 10 gr of imbalance with the 4 x 280 mL rotor equipped with standard buckets Carefully balance the sample load to avoid actuating the imbalance detection system 1 8 Relative Centrifugal Force Relative Centrifugal Force RCF at the circumference of a rotor and...

Page 10: ...lid for CASIO HP RCF x g 1 118 R N2 N x x 1 118 x R Speed r p m 946 RCF RCF R x 946 R Radius mm RCF N x x 1 118 M RCF MR 1 118 N2 Transformations Key sequence To determine actual g achieved at a different speed RCF2 RCF1 N2 2 N2 N1 x x RCF1 N1 To determine actual speed required to achieve a different g at the same radius N2 N1 RCF2 RCF2 RCF1 x N1 RCF1 To determine actual speed required to achieve ...

Page 11: ...34 x g C3i Angle 18 407 x g Swing out 3 082 x g CR3i Max capacity Swing out 4 x 280 ml Angle 6 x 100 ml Microprocessor controlled Display High visibility digital display Memory size 5 programs direct access Program protection Recall key lock Speed Range 500 to 14 000 rpm Step 10 100 rpm Accuracy 20 rpm Timer Range 30 sec to 99 min hold position Step 30 sec to 1 min Acceleration rates 5 Braking rat...

Page 12: ...C3i CR3i Specifications 6 ...

Page 13: ...n safety and health in the workplace The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase professional handling assistance is required Unpack the centrifuge carefully removing any possible accessories and the material supplied for ordinary operations and maintenance Check the contents of the package using the packing list provided ...

Page 14: ...that this safety device enables the lid to be unlocked refer to 3 6 for manual lid opening Carefully clean the inside of the centrifugation chamber removing any packing residues In fact due to air turbulence solid particles accidentally left in the centrifugation chamber create excessive wear of the chamber itself and of the outer rotating equipment surface To install the rotor Carefully lower the...

Page 15: ...ation In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually Upon opening lift the lid by hand and observe the rotor be careful in this operation if it is still rotating close the lid and wait The protection sticker must be replaced after it has been pierced item N 85241772 En cas de nécessité pour le déverrouillage manuel du couvercle attendre 10...

Page 16: ... x 122 158 4100 2969 11175719 Set of 4 inserts 1 x 100 ml 38 x 123 161 4100 3026 11175720 Set of 4 inserts 2 x 65 ml 34 x 104 156 4100 2932 11175721 Set of 4 inserts 4 x 50 ml 28 5 x 106 132 4100 2481 11175722 Set of 4 inserts 2 x 50 ml conical 29 x 114 134 4100 2518 11175723 Set of 4 inserts 5 x 38 ml 25 5 x 102 157 4100 2950 11175724 Set of 4 inserts 5 x 25 ml 24 x 104 155 4100 2917 11175725 Set...

Page 17: ...92 9000 8331 11174715 Set of 6 adaptors 1 x 30 38 ml 25 5 x 92 88 9000 7969 11174716 Set of 6 adaptors 1 x 15 ml conical 17 x 122 93 9000 8422 11174717 Set of 6 adaptors 2 x 10 ml 16 x 80 83 9000 7516 11174718 Set of 6 adaptors 4 x 1 5 2 ml 11 x 39 79 9000 7154 11175754 AC 50 10 Angle rotor 6 x 50 ml con round 29 5 x 118 112 10000 12520 40 11174606 Set of 4 adaptors 1 x 15 ml conique 17 5 x 122 11...

Page 18: ...C3i CR3i Installation 12 ...

Page 19: ...e set 9 Deceleration rate set 10 Stop Run 11 Pulse Run 12 Start Run 13 Temperature set R only Display Screens 15 Upper Screen speed RCF 16 Middle Screen time raduis 17 Lower Screen accel decel rates temp R models LED Lights 1a Program 1 Indicator 2a Program 2 Indicator 3a Program 3 Indicator 4a Program 4 Indicator 5a Program 5 Indicator 8a Acceleration Indicator 9a Deceleration Indicator 13 Imbala...

Page 20: ...cceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed In addition it shows the temperature in the bowl of the centrifuge When the centrifuge is at rest the set bowl temperature is displayed C During a run the actual bowl temperature is displayed C Function Start cycle Stop cycle immediate P...

Page 21: ...control display function to be accurate The correct value of the centrifugal radius depends on the combination of rotor buckets adapters being used When the button 8 is pressed the lower display 17 shows the number corresponding to the acceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down arrows When the button 9 is pressed the low...

Page 22: ...play then by pushing the up or down arrow Set run time can be changed by pressing the up or down arrow corresponding to the middle display Set deceleration profile can be changed by first selecting the deceleration profile and then using the up down arrows Stop and braking deceleration phase When the stop button is pressed or when the run timer reaches zero the centrifuge will begin to decelerate ...

Page 23: ...he one diametrically opposite Imbalance of the rotor may cause major damage to the rotor and centrifuge Do not attempt to introduce liquids into rotor pockets or into tubes already in the pockets Distribute the load correctly Loads should be inserted two by two samples of equal weight at 180 degrees from the rotation axis and at equal distance radius from the center of rotation see pictures When u...

Page 24: ...ce the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides Also remember that the pins must be softly lubricated Inadaquate lubrication of the pins may cause incorrect swinging of the...

Page 25: ...ering with the electronic and mechanical parts are dangerous operations Never try to bypass the lid lock safety while the rotor is spinning Do not try to open the lid until the display returns to the stand by mode Only use a correctly grounded power source Use only rotors and accessories designed for use in these centrifuges Pay special attention to the following Installation of the unit proper ve...

Page 26: ...ainment through worldwide recognized testing procedures performed in recognized external laboratories Aerosols are produced naturally when using uncapped tubes or bottles They are also produced when a container deforms or breaks provoking a small or large sample leak We draw your attention to the use of tubes in angle rotors Even if the liquid level does not touch the cap when in a static state it...

Page 27: ...o be used in medical research where hazardous substances including radioactive chemicals are frequently found ALWAYS USE THE APPROPRIATE DECONTAMINATION PROCEDURES WHEN THE ROTOR IS EXPOSED TO THESE CHEMICALS Examples of commonly used techniques are outlined below The information is given as a guideline only It is the responsibility of the owner to use the most suitable procedure If decontaminatio...

Page 28: ...ls are very corrosive and should never be used 6 4 Radioactive Decontamination We recommend that all radioactive contamination be referred to your Radioactivity Safety Officer Rotors may be decontaminated by a mixture of equal volumes of Distilled water SDS diluted to 10 Ethanol diluted to 70 The rotor should then be rinsed with ethanol followed by distilled water and then dried completely Thermo ...

Page 29: ...curved face of the trunnion Do not apply too much grease because it will eventually coat the bowl of the centrifuge as centrifugal force pulls the grease from the trunnions If the centrifuge is having imbalance problems try this operation before calling Thermo service In many cases imbalance problems arise due to the fact that poorly maintained bucket trunnion interfaces prevent the buckets from s...

Page 30: ... O O O O O S O S S U S S O O S O S O O Aluminum Hydroxide S O O O O O O O S Mt Sd S S O O O O O O Aluminum Nitrate M O O O O O O O S O O S O O O O O O O Aluminum Sulphate S O O O O O O O Sd O S Sd O O O O O O O Amino Acids S O O O O O O O S S S S S O O O O O O Ammonia S O O O O O O O S U S S Sd O O O O O O Ammonium Acetate O O O O O O O O S S S S O O S S S O O Ammonium Carbonate S U S S O S O S S ...

Page 31: ...Chlorobenzene O O U U O S O O U U U U O O S O S O O Chloroform X U M S M S O U U U U U U S S M S S S Chlorosulphonic M O O O O O O O M O M M O O O O O O O Chromic Acid 5 U O O O O O O O S M S S U O O O O O O Chromic Acid 10 M U U U U S S O S M S S U U S S S S M Chromic Acid 50 U U U S U S O O D U S S U U S S M S M Cinnamon Essence O O O O O O O O U St U U M O O O O O O Citric Acid 10 S S S O M S S...

Page 32: ... Acid 100 S M U O U S S U S M S S O U S S S U S Freon TF U O O O O O O O Sd Sd Sd Sd Sd O O O O O O Fuel Oil O O O O O O O O Sd Sd Mt Sd Sd O O O O O O Glucose S O O O O O O O S O S S O O O O O O O Glutaraldehyde O O O O O O O O Sd Sd S Sd Sd O O O O O O Glycerine S O O O O O O O S S S S S O O O O O O Glycerol S O O S O S S O S S S S S S S S S O O Heptane S O O O O O O O M O S M O O O O O O O Hexa...

Page 33: ...zone O O O O O O O O Sd Sd S Sd S O O O O O O Paraffin S O O O O O O O S O S S O O O O O O O Pentane S O O O O O O O U O U U O O O O O O O Perchlorethylene S O O O O O O O U U U U U O O O O O O Perchloric Acid X O O O O O O O St U St St U O O O O O O Perchloric Acid 10 U O O O U S O O S U M M O U S S S S S Perchloric Acid 70 X O O O O O O O M U M M U O S S O O O Petrol S O O O O O O O St M St St M...

Page 34: ...O O O Sodium Sulphide S S S O O S O S S U S S O S S S M S S Stearic Acid S O O O O O O O S Sd S S S O O O O O O Sulphuric Acid 10 M U S S U S S S S M S S S U S S S S S Sulphuric Acid 20 U O O O O O O O Sd Sd S Sd S O O O O O O Sulphuric Acid 50 U U U U U S S U S S S S S U S S M S M Sulphuric Acid conc U U U U U S O U D U M D U M S S U S U Sulphuric Anhydride dry or moist S O O O O O O O S Sd S S S...

Page 35: ...elements of the product that may become noxious during the life of the product are the following Electric batteries and accumulators situated in the front panel of the machine among the other electrical and electronic components Oil in the compressor of the refrigeration system Refrigerant Disposal of these elements must be in accordance with applicable regulations and recommendations defined by t...

Page 36: ...ation Certificate with all the information required Attach this Certificate to the instrument or part before sending it to Thermo Service or other authorized technical service SAT and CSAT personnel will not accept to work on instruments deprived of this Decontamination Certificate If an instrument is received at our Service facilities and in our opinion is a radioactive or biological hazard the i...

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