Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 157 / 204

Release Adherent Cells
The purpose of this task is to release cells from the bioreactor membrane, leaving the cells in the
IC circulation loop. This task includes four separate steps:
•
Step 1: performs the IC EC Washout task in preparation for adding a reagent. For example, the
system replaces IC media and EC media with PBS to remove protein, Ca++, and Mg++ in
preparation for adding trypsin.
• Step 2: loads a reagent into the system until the bag is empty.
• Step 3: chases the reagent into the IC circulation loop.
• Step 4: mixes the reagent within the IC circulation loop.
Table 12-1 describes the types of solution that are needed to attach to each line when performing Release
Adherent Cells. These solutions and corresponding volumes are based on the factory default settings for
this task.
As a precondition before starting this task:
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
Table 12-1: Solutions for Release Adherent Cells
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent Trypsin As desired
IC Media None N/A
Wash PBS 1.5 L
EC Media None N/A
Release Adherent Cells pathway:
Task
>
Release and Harvest
>
Release Adherent Cells
1. Confirm the settings for step 1 shown in Table 12-2.
Table 12-2: Step 1 Settings for Release Adherent Cells
Setting Factory Default Setting Options
IC Inlet Wash Reagent, IC Media, Wash, EC Media
IC Inlet Rate 100 mL/min N/A
The system automatically calculates this rate based on the EC Inlet Rate
and the number of IC Volumes and EC Volumes used for the Exchange
stop condition for this step.
Release and Harvest Tasks
12-2 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1