Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 147 / 204

Add Bolus
The purpose of this task is to quickly add a selected volume of reagent into the IC circulation loop; you
can also add a bolus into the EC circulation loop at the same time.
Table 11-5 describes the types of solution that are needed to attach to each line when performing Add
Bolus. These solutions and corresponding volumes are based on the factory default settings for this task.
Table 11-5: Solutions for Add Bolus
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent Reagent 0.1 L
IC Media None N/A
Wash None N/A
EC Media None N/A
Add Bolus pathway:
Task
>
Feed and Add
>
Add Bolus
1. Confirm the settings for Add Bolus shown in Table 11-6.
Table 11-6: Settings for Add Bolus
Setting Factory Default Setting Options
IC Inlet Reagent Reagent, IC Media, Wash, EC Media, None
IC Inlet Rate 10 mL/min 0 to 200 mL/min
IC Circulation Rate 100 mL/min −300 to 300 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet None Reagent, IC Media, Wash, EC Media, None
EC Inlet Rate 0 mL/min 0 to 300 mL/min
EC Circulation Rate 30 mL/min 0 to 300 mL/min
Outlet EC Outlet IC Outlet, EC Outlet, Harvest
Rocker Stationary (0°) In Motion (−180° to 270°, 0 to 3600 sec), Stationary
(−180° to 270°)
Feed and Add Tasks
11-6 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1