Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Feed Cells
The purpose of this task is to continuously add fluid at a low flow rate to the IC circulation loop and/or
the EC circulation loop. There are several outlet settings that you can use to remove the fluid added to
the system during this task.
Table 11-3 describes the types of solution that are needed to attach to each line when performing Feed
Cells. These solutions and corresponding volumes are based on the factory default settings for this task.
Table 11-3: Solutions for Feed Cells
Line Solution Attached to the Line
Required Volume (estimate
based on factory default
settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein 6 mL/hour
Wash None N/A
EC Media None N/A
Feed Cells pathway:
Task
>
Feed and Add
>
Feed Cells
1. Confirm the values for each setting for Feed Cells shown in Table 11-4.
Table 11-4: Settings for Feed Cells
Setting Factory Default Setting Options
IC Inlet IC Media Cell, Reagent, IC Media, Wash, EC Media, None
IC Inlet Rate 0.1 mL/min 0 to 10 mL/min
IC Circulation Rate 20 mL/min −100 to 100 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet None Reagent, IC Media, Wash, EC Media, None
EC Inlet Rate 0 mL/min 0 to 10 mL/min
EC Circulation Rate 30 mL/min 10 to 300 mL/min
Outlet IC Outlet EC Outlet, IC Outlet, Synchronization
Rocker Stationary (0°) In Motion (−180° to 270°, 0 to 3600 sec), Stationary
(−180° to 270°)
Feed and Add Tasks
11-4 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1