CSB CryoEM Facility TF20 Operating Instructions SEC
05/02/16
IV.
End of Session
1.
Close column valves
2. Retract the campera
3. Put the screen down
4.
Home the stage (go to SearchStage and select the tab Navigate to Control and click on
Holder, this ensures that the stage is at 0um in X, Y and Z.
5.
Remove the holder carefully.
6.
Click on cryo cycle. Make sure the cryo cycle will run for 365 minutes or more.
Wait ~5 minutes or for Turbo to turn yellow before removing the liquid nitrogen dewar
7. Dismount the specimen from the holder and dispose of it (or save it) properly.
8.
Once the Turbo turns yellow, remove the dewar from the microscope and dispose of the
remaining LN2 in the foam container under the table.
9.
Place the protective fabric underneath the anticontaminator.
10.
If using the RT or RT tomography holders, return them to their proper receptacle.
11.
If using the Gatan cryo holder(s), return to the dry pumping station and follow the instructions
there on how to warm it and pump it properly.
12.
Please sign up on the user's sheet. Record the actual time spent imaging.
13. Return the next day to retrieve Data. Data will be moved overnight to the computer located
behind the curtain in the F20 room
V.
Imaging
1. Open Digital Micrograph
2. Set exposure time to 1 second
3. Want the mean value to equal ~1000