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Techne Touchgene Gradient, Operator'S Manual

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Techne Touchgene Gradient Operator'S Manual Download Page 37

T o u c h g e n e   G r a d i e n t   O P E R A T O R ’ S   M A N U A L

PAGE 37

Accessories

The following accessories can be obtained from Techne or your Techne dealer:

Item No

Description

Quantity

FTU05TW 0.5ml microtubes, thin wall

Pack of  1000 with caps

FTU02TW 0.2ml microtubes, thin wall

Pack of  1000 with caps

FMITUBC 0.2ml microtubes, thin wall

125 strips of 8

FMICAPC 0.2ml micro tube caps

125 strips of 8

F96T02

96 well plate, flat top non-skirted, pad of 25 plates

pack of 25

F96PLNS

96-well plate raised top non-skirted

Case of 50

F96PLHS

96-well plate half skirt

Case of 50

F96SEAL

96-well sealing mat (silicone)

Case of 50

FGRAD02 Gradient block 96 x 0.2ml microtube or 96 well plate

1

FGRAD05 Gradient block 40 x 0.5ml microtube

1

FGRADF

Flat plate block for in situ (non gradient)

1

For full details of “Gensoft” contact your dealer or Techne Sales Department.

Replacement parts

The following replacement parts can be obtained from Techne or your Techne dealer:

Item Nº

Description

Quantity required

FCABRTUK

Mains cable and plug UK 230V

1

FCABRTEU

Mains cable and Schuko plug 230V

1

FCABRTUS

Mains cable and US 3-pin plug 120V

1

FCABRTUS

Mains cable and US 3-pin plug 100V

1

6500342

Fuse T6.3A, 230V units

2

6008445

Fuse T8A, 120V units

2

6500324

Fuse T10A, 100V units

2

6104172

Non-Volatile Memory Module

1

Summary of Contents for Touchgene Gradient

Page 1: ...1 Block removal 11 The heated lid 12 The memory card 13 Adjusting the light intensity of the screen 13 Before switching on 14 Symbols on the screen 14 POWER UP SCREENS 15 System user main menu 16 Program defaults 16 Numerical input screen 17 PROGRAMS 18 New program screen 18 Template program screens 19 Existing program screen 21 Editing programs 22 The Gradient 26 Increment or decrement temperatur...

Page 2: ...T o u c h g e n e G r a d i e n t O P E R A T O R S M A N U A L PAGE 2 ...

Page 3: ...EN 61010 2 010 1995 Particular requirements for laboratory equipment for the heating of materials I have made all reasonable enquiries regarding the unit stated and its conformance to the following EU directives Low Voltage directive 73 23 EEC and amendment 93 68 EEC and EMC Directive 89 336 EEC and amendments 91 263 EEC 92 31 EEC and 93 68 EEC To the best of my knowledge and belief these units co...

Page 4: ...use When you have finished heating samples remember that parts of the unit the tubes blocks and associated accessories may be very hot Take the precautions listed earlier Guarantee The unit is guaranteed against any defect in material or workmanship for the period specified on the enclosed guarantee card This period is from the date of purchase and within this period all defective parts will be re...

Page 5: ...ur un plan de travail ou surface plane ou le cas échéant dans une hotte d aspiration en s assurant que les trous d aération situés sous l appareil ne sont pas obstrués 5 Les symboles situés sur ou à côté de l interrupteur de l appareil ont la signification suivante I arrêt O marche Après utilisation Lorsque vous avez fini de chauffer les échantillons n oubliez pas que certaines parties de l appare...

Page 6: ...sfläche bzw falls erforderlich unter einen Laborabzug Beachten Sie daß die Entlüftungsrippen an der Geräteunterseite immer frei zugänglich sind 5 Die Symbole auf oder neben dem EIN AUS Schalter an der Geräterückseite bedeuten I An O Aus Nach dem Gebrauch Vergessen Sie nicht daß Teile des Gerätes die Gefäße die Blöcke und andere Zubehörteile nach dem Erhitzen von Proben noch sehr heiß sein können B...

Page 7: ...cluso en una campana con extractor de humos asegurándose de que las entradas de aire en la parte inferior no queden obstruidas 5 Los símbolos que se encuentran en o cerca del interruptor de alimentación tienen los siguientes significados I Interruptor principal encendido O Interruptor principal apagado Después de su uso Cuando haya finalizado el calentamiento de muestras recuerde que las piezas de...

Page 8: ...nal programs may be stored in the nonvolatile memory cards which are plugged into a slot under the cover above the screen A programme which can be recalled from memory consists of a series of specified temperatures in C the times for which each specified temperature will be held Hold Times the desired heating or cooling rates in C min between each specified temperature whether the times and or the...

Page 9: ...p time from ambient to 105 C 4 min Over temperature cutout Yes Regulated lid pressure Yes Programs Password protected Yes Incremental decremental Segment Hold Time 99 to 99sec 1sec intervals Incremental decremental Temperature 5 5 C to 5 6 C in 0 1 C intervals Variable programmable ramp rate steps 0 1 C sec Programmable ramp rate resolution 0 1 C Gradient calculator Yes Maximum programs internal m...

Page 10: ...tions including DNA sequencing and PCR Aspects of the PCR process are claimed in U S Patent Nos 4 683 195 4 683 202 and 4 965 188 Use of the Touchgene Gradient in such processes does not convey a licence to practice the processes themselves Tubes or Reaction Vessel Techne does not recommend any specific tube or reaction vessel other than those described in this Manual We recommend using reaction v...

Page 11: ...om Decon Laboratories Ltd suitable for nonferrous metals should be used but remember other parts of the unit are made of ferrous materials and may be damaged by spillage onto them You can clean the case or the screen of the Touchgene Gradient with a cloth dipped in water or ethanol methanol or formaldehyde can also be used No part of the case or cover should be immersed in the solvents Do not use ...

Page 12: ... shut To adjust the pressure on the samples Adjust the red rotating knob anticlockwise to give minimal pressure shut the lid and latch it Gently rotate the knob clockwise until you can just feel pressure beginning to be applied Rotate the knob a further quarter of a turn and this will give the correct pressure Do not over tighten the knob as this will cause deformation of the silicone rubber membr...

Page 13: ...ades of the control software Either via copying software from a new unit and transferring it to an older unit or by obtaining a copy of the new software from Techne This is a relatively easy process see the System Administration card and means that you can keep your unit up to date with the latest software Adjusting the Light Intensity of the Screen The light intensity of the screen can be adjuste...

Page 14: ...k was and you will see a label as shown In the centre of the label is a switch When the unit leaves the factory the switch is set to 230V to avoid accidental damage Ensure that the appropriate fuses are fitted for your supply For 230V set the voltage selector switch set to 230V and fit T2 5A fuses For 115V set the switch to 115V and fit T4A fuses For 100V units the switch will be set to 115V T4A f...

Page 15: ...atures are temperatures which you require the sample to achieve Displayed temperatures are either the temperatures measured by the block probe the calculated sample temperature or both The screen shown is displayed when the unit is powered on and will remain displayed while the unit performs it s self tests After the self tests this screen is displayed unless the unit is powered up by auto restart...

Page 16: ...ttings for the programs you are about to put into the unit If you touch the button for a field where there is either ON or OFF then the field will toggle between the two For example Pause before program Off One touch gives Pause before program On A second touch will return the field to the first example If you touch a button where there is a numerical input it will transfer you to an input screen ...

Page 17: ...s hours minutes or seconds The arrow buttons only operate where you need to change fields The Disable button puts xxxxx in the fields The button itself will change to Enable If the segment was previously Disabled the reverse will happen and you will be able to put numerical values in the field The Cancel button takes you back to the previous screen without any changes The BS button is a back space...

Page 18: ...emory card or remotely how to edit and use existing programs are explained on page 21 Use the or down arrow or up arrow key to toggle through the programs Select any one by touching the appropriate field If you touch Exit you will return to the System User Main Menu New Program Screen If you choose New then you will go into an input screen to give the program a name Once you have entered the name ...

Page 19: ... Program screen if you touch 2 step PCR template you will come to this screen Touch Copy and you will go into the title screen Type in your name for the program and touch Enter If you try to Edit any of these programs you will find that you can only Save as when you finish The Save key will not be on the screen If you touch Delete you will be asked if you are sure you want to delete the program Se...

Page 20: ...o 15 0 C Program 3 STEP PCR TEMPLATE Hot Start Disabled Number Cycles 30 Seg MAX c m 00m30s 92 0 c Seg MAX c m 00m30s 55 0 c Seg MAX c m 00m30s 72 0 c Final extension 05m00s 72 0 c Final Hold 10 0 c Copy Delete Edit Run Print Exit Program ICE BUCKET Heated Lid Disabled Heated Lid before program Off Pause before program Off Initial denaturation Disabled Hot Start Disabled Final extension Disabled F...

Page 21: ...een If you select an existing program from the Programs screen you will get a screen similar to this one Touch the down arrow button to toggle more parts of the program You can then toggle back using the up arrow button If you touch Delete you will be asked if you are sure you want to delete the program Select Yes or No Before you touch Print make sure that you have a printer connected to the Touc...

Page 22: ...s 3 Seg MAX C m 00m30s 92 0 c 10 c Seg MAX C m 00m30s 92 0 c Next step Final extension Disabled Ins Del Edit PWord Save As Save Exit Editing Programs From the Program screen touching Edit puts you into a very similar screen The top field will have gone and the next field will have appeared at the bottom of the set of seven fields as shown in this picture Password protection If you do not want othe...

Page 23: ... Disabled you will get a Numerical Input screen when you touch the Edit button with the field highlighted Editing Bill Heated Lid Disabled Heated Lid before program Off Pause before program Off Initial denaturation Disabled Hot Start 05m00s 94 0 c Number Cycles 3 Seg MAX C m 00m30s 92 0 c 10 c Ins Del Edit PWord Save As Save Exit Editing Bill Initial denaturation Disabled Hot Start 05m00s 72 0 c N...

Page 24: ... you can use the Next step that the program inserts automatically 3 Touch the Next Step field and highlight it 4 Touch Edit You will now be in the Edit Program Function screen Editing Bill Initial denaturation Disabled Hot Start 05m00s 72 0 c Number Cycles 3 Seg MAX C m 00m30s 55 0 c 10 c Seg MAX C m 00m30s 92 0 c Next step Final extension Disabled Ins Del Edit PWord Save As Save Exit 1 3 2 4 Edit...

Page 25: ...ht Arrow key to move to the Ramp field Type in the Ramp Rate as appropriate The Ramp rate you put in here will be the rate from the temperature at the begining of the Segment to the temperature of the mid point of the Gradient Rates to the highest and lowest temperatures will be adjusted appropriately Edit program function Seg 75 0 c 0 c 00m30s MAX c m Segment h 7 8 9 Number m 4 5 6 Continue Cycle...

Page 26: ... 20 C and the hottest temperature is 70 C However you must remember that the greater the gradient the poorer the uniformity The illustration shows a Set Temperature of 55 C with a Gradient of 10 C on a 0 2ml x 40 block Other examples Set temperature of 55 C with a gradient of 20 C will give a low of 45 C and a high of 65 C a set temperatureof 50 C with a gradient of 16 C will give a low of 42 C an...

Page 27: ...es to suit Inc Dec time or temperature You can insert an incremental value for Time or Temperature and these are illustrated on the next page Touch Inc Dec Edit program function Next Step Segment h 7 8 9 Number m 4 5 6 Continue Cycles Inc s 1 2 3 Cancel Dec Pause 0 BS OK Then First and or Edit program function First 75 0 c 0 c 00m30s MAX c m Last 75 0 c 0 c 00m30s MAX c m Segment h 7 8 9 Number m ...

Page 28: ...ented decremented only subsequent Cycles If you select a decrement the Hold Temperature is prevented from falling below 4 C If you select an increment the Hold Temperature is prevented from rising above 99 C Similarly under normal circumstances the Hold Time of all segments is constant However it is possible to automatically increment or decrement the duration of a specified segment of a programme...

Page 29: ...g MAX c m 00m35s 75 0 c 0 c Ins Del Edit PWord Save As Save Exit Completion Touch OK when you have the values you need in each of the fields You will return to the Editing screen Save before Run Once you are satisfied that the information is correct you must Save the program Touch Save Alternatively you can give the program a different name by touching Save As and following the on screen instructi...

Page 30: ... 10 1998 22 48 11 Auto Restart Enabled Password Protection Disabled Heated Lid Enabled Initial Denaturation Disabled Hot Start Disabled 01 Cycles Set Point C RampRate C HoldTime Segment 4 0 Max 00 10 00 03 Cycles Set Point C RampRate C HoldTime Segment 95 0 Max 00 00 30 Segment 55 0 Max 00 00 30 Segment 72 0 Max 00 01 00 either or RUNNING A PROGRAM From the Select program screen you will go into t...

Page 31: ...nd so initially you will have a blank graph with two axis lines The trace will consist of one or two lines the block temperature the sample temperature or both this depends on the selection you made in Program Defaults If you wish to pause the program touch the Pause button Press Unpause and the program will continue If you wish to stop the program you have to press Stop twice This is to prevent a...

Page 32: ... program is complete from the System User screen choose Data Logs Select the Data Log that you want to print by highlighting it Make sure that the printer is plugged in and set on line before pressing the print key Note that profiles cannot be printed out during calibration The events which are used to generate profile information are Start A start of program event is generated on first pressing t...

Page 33: ...39 56 02 01 03 55 0 C 72 0 C MAX C m Holding 30 11 1998 14 40 05 02 01 03 65 5 C 72 0 C Ramping 30 11 1998 14 41 05 02 02 01 72 0 C 95 0 C MAX C m Holding 30 11 1998 14 41 16 02 02 01 88 5 C 95 0 C Signed Typical Data Log Printout Data Log NORMAN Program Name DEPT PROGRAM Time Started 30 11 1998 14 27 26 Temperature C 0 10 20 30 40 50 60 70 80 90 Time m 0 5 10 20 Typical Graph from a Data Log A pr...

Page 34: ... each individual unit and the time remaining F Print out of the protocol in a diagrammatic format together with associated notes page If you are driving one unit only you will need FGEN232 An RS232 cable set which includes a cable the software on disc and an instruction sheet If you are driving two or more units then you will need FTG485D A Computer Connection set This set includes the connection ...

Page 35: ...are lit one of the two fuses may have blown Check that there is no external cause such as a faulty plug or lead Check both fuses and replace the faulty fuse with a new one of the correct value Fuse values are given on the label next to the power inlet Note that fuses should only be replaced by a qualified electrician The holder for the two fuses is built into the mains input socket First remove th...

Page 36: ... the block by one quick release handle always use both handles or support the block underneath When refitting the block or fitting a new one slide the quick release handles in the opposite direction to the above arrows to lock the block assembly into the unit The software will register the change and calibrate the unit for the new block Remember to adjust the options in the Program Defaults Menu f...

Page 37: ...e half skirt Case of 50 F96SEAL 96 well sealing mat silicone Case of 50 FGRAD02 Gradient block 96 x 0 2ml microtube or 96 well plate 1 FGRAD05 Gradient block 40 x 0 5ml microtube 1 FGRADF Flat plate block for in situ non gradient 1 For full details of Gensoft contact your dealer or Techne Sales Department Replacement parts The following replacement parts can be obtained from Techne or your Techne ...

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Page 40: ......

Page 41: ...Gradient Gradient Gradient OPERATOR S MANUAL Issue F Date of issue 26th September 2000 Touchgene is the registered trademark of Techne Incorporated Princeton NJ USA The Techne logo is the trademark of Techne Cambridge Limited and Techne Inc Techne Cambridge Limited 2000 ...

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