4. Optical System
42
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
4.3.1 Absorbance
Optics
Up to 4 fiber bundles guide the light from the excitation monochromator system to
the absorbance optics (Figure 17). The absorbance optics consist of a pair of
lenses which focus the light beam into the well of the microplate (Figure 18).
Figure 18: Absorbance Optics, one channel.
The absorbance channels are spaced to read a 96-well plate. The optical
alignment for a 384 or 1536-well plate is shown in Figure 19.
The software automatically sorts the data and reports it in the correct order. Each
well is measured with only one channel in absorbance mode. If a plate type other
than 96/384/1536 is used, only a single optic channel will be used for the
absorbance reading.
The
light beam diameter
of the absorbance optics is
about 1 mm
.
Measurements using the injector are performed with one absorbance channel
only.
Figure 19: From left to right: Schematic view of the channel usage of an
absorbance measurement of a 96, 384, 1536 and 6-well plate.
