4. Optical System
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
41
4.3 Absorbance
System
For absorbance measurements, a similar optical path is used as for fluorescence
excitation. For details of the light source (1) and the excitation monochromator
(2), please refer to chapter 4.1.1 Light Source System Fluorescence Intensity.
A fiber bundle guides the light from the excitation monochromator (2) to the
absorbance optics (3), which focus the light into the wells. The transmitted light is
measured by silicon photodiodes (4) located beneath the plate carrier (see figure
below).
Before the measurement of the microplate is performed, a reference
measurement is made with the plate carrier moved away from the light beam.
Figure 17: Optical System Absorbance.
1)
2)
3)
4)
