CONTROLLED COPY
Centre for Biological Engineering
Standard Operating Procedure
SOP025
Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES
Location:
Autoclave Room, H31, CBE
Version 004
Effective Date: 28
th
June 2020
Review 28
th
June 2022
Written by: A. Chandra
Reviewed by: P.Hourd
Approved by: R.I.Temple
Page 5 of 26
Mastercopy
(vi)
Principal investigators/supervisor’s MUST ensure that his/her employees are trained before
operating any autoclave unit. All operators (regardless of experience) must have successfully
completed an authorized training session on the safe operating procedures of autoclaves.
Users must be instructed on the operation and safety regulations and, if required, to repeat this
instruction at regular intervals
(vii)
Maintenance of autoclaves should not commence without a 'Decontamination Certificate'.
4. EQUIPMENT AND MATERIALS
1) Systec VX-95 Autoclaves Number CBE045 and CBE 044
2)Printer
3)De-ionised water vessel
4)Autoclave Basket
5)Autoclave Bucket – item J807 from nisbets.co.uk
6)Integrated hoist
7)Autoclave Log Sheets
8)Autoclave bags (Fisherbrand, polypropylene or HDPE)
9)Autoclave Indicator Tape
10)Polypropylene or stainless steels tubs as secondary containment
11)Sharpsafe one Sharps autoclavable containers (nominal volume – 1L),
5. PROCEDURE
The following describes procedures for (i) the validated steam sterilization of solid form biological waste
that may contain biological agents or Genetically Modified Organisms. (ii) The sterilization of solids,
reusable equipment and (iii) the sterilization of liquids, aqueous solutions and media.
In addition to this SOP and the Operators manual, simple, easy to understand instructions are
provided for the operator in the autoclave room.
NOTE;
before proceeding all operators must don
o
Full length apron (green)
o
Eye protection (safety goggles or full face visor)
o
Specialised Heat Resistant (Orange) Gloves
