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3.
Load a micropipette (appropriate to the chosen application) into the micropipette holder
and install it in the clamp of the mechanical so that the tip of the micropipette is pointing
in and down towards the optical axis of the microscope.
4.
Adjust the angle setting of the micropipette clamp on the mechanical so that the angle of
attack of the pipette is appropriate for the chosen application as described in Operating
Instructions.
5.
Once an appropriate angle of approach has been set, loosen the micropipette holder clamp
slightly and slide the holder inwards along its own axis, until the micropipette tip is level
with (almost touching) the plane of the microscope stage.
NOTE: Do this by eye – it is not necessary to look down the microscope at this stage.
6.
Place the manipulator in ‘Coarse’ mode ‘C’, and carefully manipulate the joystick until
the micropipette can be seen through the microscope eyepieces.
7.
Change the speed to 1 or 2, center the micropipette tip in the microscope field of view, and
change microscope to a higher magnification. Remember not to adjust the microscope
focus at this point. To “focus” the micropipette tip, move it up and down in the Z-axis by
rotating the Z-axis dial at the bottom of the joystick.
8.
Once the desired magnification has been reached, and the micropipette is still in view, set
the ‘Work’ position.
9.
To replace the microinjection dish, press ‘Home’ to take the micromanipulator to the
‘Home’ position (make sure that a ‘Work’ position has been set first). Once the
micropipette tip is clear of the microscope stage, replace the microinjection dish. Pressing
the ‘Work’ button will cause the micropipette tip to return to the ‘Work’ position
previously set.
10.
At this point, it may be desirable to set the ‘Z-floor’ function to prevent accidentally
breaking the micropipette during use. To do this, lower the tip to the bottom of the dish
and you will see the micropipette tip “kick-out” slightly as it touches the dish (this is not
recommended for sharp micropipettes with a high angle of attack, since the micropipette
tip may break). Press and hold the ‘Z-floor’ key until a tone is heard and the green LED
illuminates. The ‘Z-floor’ is now active. The ‘Z-floor’ will stay in the micromanipulator
memory until it is overwritten with a new limit or until the instrument is powered down.
11.
The XenoWorks™ Micromanipulator is now ready for microinjection.
Note that, as the system becomes more familiar, many of these steps (like the Z-limit) can be
skipped.
XENOWORKS MICROMANIPULATOR SYSTEM (TOUCHDECLUTCH) OPERATION MANUAL – REV.
