strex STB-150 User Manual Download | Manualshive

Page: 1 / 16

background image

STREX Cell Strain Instrument

Cat. # STB-150

User Manual

Cell Stretching System

Model # STB-190-XY

User Manual

Strex US Office

10060 Carroll Canyon Rd., Suite 100

San Diego, CA 92131

Email:

[email protected]

www.strexcell.com

1
2
3
...
»

Summary of Contents for STB-150

Page 1: ...ell Strain Instrument Cat STB 150 User Manual Cell Stretching System Model STB 190 XY User Manual Strex US Office 10060 Carroll Canyon Rd Suite 100 San Diego CA 92131 Email info strexcell com www strexcell com ...

Page 2: ...s mounted on four brackets Fan Chamber Length Adjustment Knob To freely rotate the knob the power switch must be OFF Use the knob to adjust the distance of the chamber brackets such that it maintains tension on the silicone chamber Motor Cable Cable connects to Control Unit Platform Place platform ontop of microscope stage for viewing ...

Page 3: ...top Button Use to start or stop the stretching action Stretch Ratio Selector right digit Use upper and lower buttons to increase and decrease strain ratio Control Unit Front Panel Control Unit Back Panel Motor Cable Outlet Connects the Stretch Unit to the Control Unit via Motor Cable Fuse Power Cable Outlet ...

Page 4: ... 2 Connect the Control Unit to the power supply 240 V by the Power Cable 3 Turn on the Power Switch to start operation The Power Switch will light up and the Stop Button will flash BEWARE The Fan that cools the step motor will be rotating Do not obstruct the fan blades with any objects 4 If the electrical system is operating properly turn OFF the Power Switch 5 Make sure that the microscope is lev...

Page 5: ...ensity in the chambers For example epithelial cells often form a cell sheet and the cell cell adhesion seems to be stronger than a cell surface adhesion When this happens cells may detach from the chamber Additionally cultures that are grown over a week in the chambers may detach 2 After an overnight incubation without stretching inspect the cells with a microscope to ensure they have adhered to t...

Page 6: ... Pour 3 ml of the gelatin solution into each strain chamber 2 Incubate at 37oC for more than 30 minutes 3 Aspirate the gelatin solution If coating is successful water will not be repelled after removing the gelatin solution 4 The liquid solution can be used to coat 3 or 4 chambers before discarding Collagen Coating Cellmatrix 1 C P Type 3 or 4 Preparation of collagen solution 1 Combine 1 part coll...

Page 7: ...contract 0 5 second end 4 1cycle min Square wave Stretch 0 5 seconds hold 29 5 second contract 0 5 second hold 29 5 seconds repeat 5 6cycle min Square wave Stretch 0 5 seconds hold 4 5 second contract 0 5 second hold 4 5 seconds repeat 6 20cycle min Square wave Stretch 0 5 seconds hold 1 second contract 0 5 second hold 1 seconds repeat 7 60cycle min Square wave Stretch 0 5 seconds contract 0 5 sec...

Page 8: ...n layer of ethanol between the dish and the chamber will remove any wrinkles in the chamber membrane Allow the ethanol to evaporate before spreading your cell suspension in the chamber Q3 Cell attachment on the stretch chamber was confirmed by microscopy But the cells detached from the chamber surface after stretching the cells A3 Try seeding your chambers at a lower concentration of cells In typi...

Page 9: ...fer directly into the chamber and collect the cell extract by using a cell scraper 3 RNA Wash the cells once with PBS for RNA preparation Add RNA extraction buffer directly into the chamber and collect the cell extract by using a cell scraper Q6 I want to use recombinant cells for an experiment A6 Direct transfection of cells in the chamber may be possible However transfection itself may damage th...

Page 10: ...othelial cells K Naruse T Yamada X Sai M Hamaguchi and M Sokabe Oncogene 17 455 463 1998 9 Orientation Change of Cardiocytes Induced by Cyclic Stretch Stimulation Time Dependency and Involvement of Protein Kinases K Kada K Yasui K Naruse and J Toyama J Mol Cell Cardio 31 247 259 1999 10 Molecular Identification of a Eukaryotic Stretch Activated Nonselective Cation Channel M Kanzaki M Nagasawa I Ko...

Page 11: ...etch induced cell proliferation is mediated by FAK MAPK pathway Life Sci 76 24 2817 2825 2005 22 Fabrication of reconfiguration protein matrices by cracking X Zhu K Mills P Peters J Bahng El Liu J Shim K Naruse M Csete M Thouless S Takayama Nature Materials 4 403 406 2005 23 Involvement of reactive oxygen species in cyclic stretch induced NF κΒ activation in human fibroblast cells H Amma K Naruse ...

Page 12: ... software not involving Strex We are not responsible for any incidental damage caused by the use or misuse of this product including loss of business income interruption of business loss of stored data theft of machine etc WARNING Please do not place water or water containing vessels on or near the machine Cups vials tubes etc containing water should not be located on or near the device Be careful...

Page 13: ...ndling of This Equipment Do not place the power cable close to a heating source such as a hotplate or open flame The cable cover may melt causing fire electric shock malfunction etc When unplugging the power cable from the outlet please do not pull on the cable part but remove at the plug Pulling the cable will damage the cable and cause fire electric shock breakdown etc Regularly check the condit...

Page 14: ...he instrument on a stable level floor or a table secure from vibrations Be sure you have enough space Do not store the instrument in a humid or dusty place Over time excessive humidity or dust may cause deterioration that can result in an electrical short circuit and possibly fire Do not use the machine in a place where the temperature is excessively high Do not place and run the machine near a he...

Page 15: ...tion In the case of any abnormal sound smell or smoke disconnect the power immediately and contact B Bridge International Do not run the machine overloaded Be cautious as to your clothing and hair when operating the instrument Baggy clothing neckties necklaces etc can get tangled in moving parts of the unit Take appropriate precautions to prevent this occurrence Keep the machine clean and periodic...

Page 16: ...warranty does not cover damage to the instrument that is a result of the following circumstances Damage caused by dropping or other impact Damage caused by inappropriate operation of the instrument Damage resulting from an attempted repair or modification of the instrument by the user Damage caused by unavoidable external causes such as earthquakes lightening fire flood gas leak power surges or ot...

Reviews:

No comments

Related manuals for STB-150

Brand: Laica Pages: 4

medialon Showmaster Go

Brand: 7th Sense Pages: 9

Brand: DB Industries Pages: 60

Brand: vc999 Pages: 55

Brand: Salsbury Industries Pages: 4

Brand: Gardena Pages: 19

Brand: Jackco Pages: 6

Brand: Garmin Pages: 2

Brand: mychway Pages: 56

Brand: U-Line Pages: 6

Brand: Joyolight Pages: 4

SynCore RW Unit

Brand: Ridder Pages: 28

Brand: TECshow Pages: 22

Brand: TBM Pages: 22

Robus RVB007RGB10I65

Brand: LED Group Pages: 2

Brand: Monosem Pages: 52

Brand: DALBO Pages: 27

Infinity Wash XL

Brand: Coemar Pages: 6

Brands by name

0 1 2 3 4 5 6 7 8 9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Popular brands

Load more brands