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STREX Cell Strain Instrument

Catalog # STB-140

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-04

User Manual

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Summary of Contents for STB-1400-04

Page 1: ...STREX Cell Strain Instrument Catalog STB 1400 04 User Manual ...

Page 2: ...in Chamber 5 Section 2 Use of the Cell Strain Instrument 6 Preparation of Cell Strain Instrument 6 6 7 System Operation Culturing Cells in the Silicone Chambers Preparation of Silicone Chambers 8 Section 3 Strain Parameters 10 Section 4 FAQ 11 Section 5 References 13 Section 6 Safety Instructions 15 Section 7 Warranty 18 ...

Page 3: ...r length adjusting knob Use the knob to adjust the distance of chamber brackets to maintain tension on the chamber The bottom of the chamber should be taut Connectors Use Connector Cable to connect Control Unit to Stretch Unit Alignment Pins Holds Strain Chamber Brackets in place Strain Chamber Brackets Each chamber is mounted on four pins two pins on each bracket Mounting Stage Brackets sit on to...

Page 4: ...wer source Strain Ratio Use upper and lower buttons to increase and decrease stretch ratio Strain Frequency Selector Use upper and lower buttons to adjust cycle frequency Water Pump Switch Use to turn on and off the pump that circulates water to cool the motor System coolant inlet outlet valves Be sure to supply cool water when in operation Failure to do so may lead to overheating inside the incub...

Page 5: ...5 Silicone Strain Chamber ST CH 04 0 10m m Chamber Dimensions 20mm x 20mm x 10mm 31m m 18m m 25m m 40m 4 places for pins Chambermembrane thickness 0 1mm ...

Page 6: ... Coolant Inlet and Outlet Valves see Section 1 To release the metal plugs from the Stretch Unit push backward on the outer metal rings around the valves The single larger black plug supplies electricity to the Stretch Unit this plug has to be in the correct orientation to fit properly The other end of the Connector Cable has 2 exposed latex tubes which fit onto the System Coolant Inlet and Outlet ...

Page 7: ...ch cycle may damage the motors 4 Push the Start Button to start the stretch unit 5 After 5 minutes stop the stretching and check the condition of the cells If the cells have not detached proceed with your experiment If the cells are detached the adhesion matrix coating was probably insufficient Recoat the chambers Culturing Cells in the Silicone Chambers 1 Seed cells at the appropriate concentrati...

Page 8: ...rging in 70 ethanol rinsing with water then drying in a sterile environment Place the sterile silicone chambers in a Petri dish in preparation for coating Fibronectin Coating Preparation of fibronectin solution 1 Dilute human or bovine fibronectin to a final concentration of 50 to 100 ug ml in Phosphate Buffered Saline PBS Coating with fibronectin solution 1 Pour 3 ml of the fibronectin solution i...

Page 9: ...be Coating with collagen solution 1 Coat chamber with a thin layer 2 Aspirate excess 3 Dry in biological safety cabinet at 25 C or below The chamber can be stored at the same temperature 4 Wash the chamber twice with culture medium 5 If coating is successful water will not be repelled The PDMS silicone chambers are very hydrophobic with two methyl bases on the surface Cells adhere to the fibronect...

Page 10: ...s min 0 5 Hz Sine wave Stretch 1 sec contract 1 sec repeat 2 15 cycles min 0 25 Hz Stretch 2 sec contract 2 sec repeat 3 10 cycles min Sine wave Stretch 3 sec contract 3 sec repeat 4 20 cycles min Square wave Hold 0 5 sec stretch 1 sec hold 0 5 sec contract 1 sec repeat 5 10 cycles min Square wave Hold 2 sec stretch 1 sec hold 2 sec contract 1 sec repeat 6 1 cycle min Square wave Hold 29 sec stret...

Page 11: ...ding your cell suspension in the chamber Q3 Cell attachment on the stretch chamber was confirmed by microscopy But the cells detached from the chamber surface after stretching the cells A3 Try seeding your chambers at a lower concentration of cells Over confluent cells generally adhere to neighboring cells rather than to the base matrix dish surface When an excess amount of cells are put in a cult...

Page 12: ...on Wash the cells once with PBS Add cell extract buffer directly into the chamber and collect the cell extract by using a cell scraper 3 RNA Wash the cells once with PBS for RNApreparation Add RNAextraction buffer directly into the chamber and collect the cell extract by using a cell scraper Q6 I want to use recombinant cells for an experiment A6 Direct transfection of cells in the strain chamber ...

Page 13: ... 615 619 1998 8 Pp125FAK is required for stretch dependentmorphological response of endothelial cells K Naruse T Yamada X Sai M Hamaguchi and M Sokabe Oncogene 17 455 463 1998 9 OrientationChangeof Cardiocytes Induced byCyclic Stretch Stimulation TimeDependencyand Involvementof ProteinKinases K Kada K Yasui K Naruse and J Toyama J Mol Cell Cardio 31 247 259 1999 10 Molecular Identification of a Eu...

Page 14: ...annel bymembranestress crated with amphipaths Mol Membr Biol 22 6 519 527 2005 21 Stretch induced cell proliferation ismediated byFAK MAPK pathway Life Sci 76 24 2817 2825 2005 22 Fabrication of reconfigurationprotein matrices bycracking X Zhu K Mills P Peters J Bahng El Liu J Shim K Naruse M Csete M Thouless S Takayama Nature Materials 4 403 406 2005 23 Involvementof reactive oxygen speciesin cyc...

Page 15: ...nnect gently by holding its plug not the cable Do not plug many objects into a single electrical outlet since it may cause fire If you are using an extension cord ensure it can withstand the total current to be used Disconnect power from the unit when it is not in use Connect the instrument to a power surge protected outlet Installation Location and Environment Keep the instrument on a stable leve...

Page 16: ... 16 Section 6 Safety Instructions and Precautions Please read this section carefully before using the instrument Items in this section alert the user to operational dangers that if not followed may damage the instrument or more significantly result in serious injury or death of the user To ensure safe operation of the instrument it is therefore imperative that you follow these instructions careful...

Page 17: ...tlet for safety 17 Section 6 Safety Instructions and Precautions Please read this section carefully before using the instrument Items in this section alert the user to operational dangers that if not followed may damage the instrument or more significantly result in serious injury or death of the user To ensure safe operation of the instrument it is therefore imperative that you follow these instr...

Page 18: ...amage resulting from an attempted repair or modiﬁcation of the instrument by the user Damage caused by unavoidable external causes such as earthquakes lightening ﬁre flood gas leak power surges or other acts of providence 3 Strex is free from any responsibility for effects or loss or damages arising from the result of the machine operation This warranty assures that Strex will repair our product f...

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