14
STEINDORFF
®
3.4.1.
Using color filters (Figure 22)
o
Selecting the appropriate color filters according your need,
it became more effective to observe or photography the
specimen. Especially, we suggest using the LBD color
filter, which can compensate more neutral colors.
o
You could pile up a group of color filters to the filter
holder, if you ensure they are level and the whole thickness
is less than 11mm.
Color filter
Meaning
IF550
Single contrast color filter (green)
(Used for the phase contrast microscopy)
LBD
Color temperature transit color filter (blue)
(Used for bright field observation and
microphotography)
3.4.2.
Using the aperture diaphragm (Figure 24)
o
When in the bright field observation, the aperture diaphragm control the numerical aperture of the
illumination system. Only when the numerical aperture of the objective and the illumination system being
matching, you can obtain the higher image resolution and contrast, and the increased depth of field, too.
To recognize the aperture diaphragm, you could remove the eyepiece if necessary. (You also could insert in
the center telescope) then looked into the viewing tube, you might see a field of view like the figure shown.
The proportion could be changed by dialing the aperture adjustment lever according your need. (
①
is the
image of the aperture diaphragm,
②
is the edge of the objective)
Generally, when observing the chromatic specimen, you need to set the size of the condenser aperture
diaphragm at 70%
～
80% of the numerical aperture which marked in the objective, but if observing the
bacterium specimen which not colored, you could turn the aperture diaphragm lever at the direction of “ ”
(clockwise).
3.4.
Illumination Unit
Figure 23
Figure 24
