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SPT Labtech Apricot S3, User Manual

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SPT Labtech Apricot S3 User Manual Download Page 19

© 2021 SPT Labtech Ltd 

 

 

 

19 

 

96 Core 

384 Core 

 

96 

Channel 

24 Ch-
O et 

8  

Channel 

4 Ch-

O et 

384 

Channel 

96 Ch-
O et 

16 

Channel 

8 Ch- 

O et 

Aspirate 

 

 

 

 

 

 

 

 

Dispense 

 

 

 

 

 

 

 

 

Empty 

 

 

 

 

 

 

 

 

Mix 

 

 

 

 

 

 

 

 

Transfer 

 

 

 

 

 

 

 

 

Serial 
Dilution 

 

 

 

 

 

 

 

 

Fill Plate 

 

 

 

 

 

 

 

 

Pooling 

 

 

 

 

 

 

 

 

 

 

96 Core 

384 Core 

 

96 

Channel 

24 Ch-
O et 

8  

Channel 

4 Ch-

O et 

384 

Channel 

96 Ch-
O et 

16 

Channel 

8 Ch- 

O et 

Reservoir 

 

 

 

 

 

 

 

 

24 Wells 

 

 

 

 

 

 

 

 

96 Wells 

 

 

 

 

 

 

 

 

384 Wells 

 

 

 

 

 

 

 

 

 
 

 

Summary of Contents for Apricot S3

Page 1: ......

Page 2: ...2021 SPT Labtech Ltd 2 Notes...

Page 3: ...ument will provide years of e ient and reliable service Failure to adhere to proper care maintenance and operation of the instrument may lead to poor experimental results reduced equipment life and th...

Page 4: ...ing the Pipetting Core 14 3 8 Personalize Operation Functions 18 Functions Available by Core Head Tips 19 Plate Head Compatibility 19 4 Safety Warning 20 4 1 Safety Precautions 20 5 Setup 21 5 1 User...

Page 5: ...4 6 2 3 Dispense Location in Well 54 6 2 4 Side Touch 54 6 2 5 Bottom Touch 55 6 2 6 Decremental Steps 55 6 3 Empty 56 6 4 Mix 57 6 4 1 Volume Retained 57 6 4 2 Mix at Corners 57 6 5 Transfer 58 6 6 S...

Page 6: ...Volume 72 7 2 Leave Plate Slow 73 7 3 Enable GLP 21 CFR Part 11 73 7 3 1 Text File Edit 73 7 4 Using CVTC 76 7 5 Change Core 76 7 6 Con ration 77 8 Pipetting Technique 78 8 1 Training Plates 78 8 2 I...

Page 7: ...at 1 L Resolution 1250 1000 500 1 L 125 0 1 L Volume Max channel capacity 1250 L Dispense range 1250 5 1000 L 1000 5 1000 L 500 1 500 L 125 0 5 125 L ParticleSize 0 032 diameter to prevent p blockage...

Page 8: ...Power cord o Access AccessDatabaseEngineFor2007up exe o This is included in the SPTLabtech folder after installation No need for further steps o Screen resolution needs to be 1920 x 1080 o Change Disp...

Page 9: ...f the top of the crate and remove any boxes and accessories Lift the sides of the crate up and off the base and remove the tool kit With the 14mm open ended wrench remove all four bolts that secure th...

Page 10: ...g the instrument be sure to lift only from the base of the instrument o Place the instrument on a steady sturdy at and level surface The instrument is now ready for installation Note We recommend that...

Page 11: ...olumn 8 16 o Automated plate elevators for plate sampling automatically raise or lower plate reservoir to programmable height o EZ Load tip technology to load disposable tips quickly and easily withou...

Page 12: ...from the pipetting mechanism allowing easy and economical recon ration in the lab Changing heads only takes a few seconds easily recon re as needed For best pipetting results consider pipette tip sel...

Page 13: ...Dilution Heads Inserts Serial Dilution Tips Strip Tips SP C 096 125 5 125 L SP 096 A 01 0125 125 096 EZ NS S FS SP 096 A 01 0125 SC 125 008 EZ NS S FS 050 096 EZ NS S FS 050 008 EZ NS S FS 015 096 EZ...

Page 14: ...system using disposable tips that do not need to be pressed on with excessive downward force in order to create a reliable seal Load disposable tips into the head quickly and easily No tools required...

Page 15: ...2021 SPT Labtech Ltd 15 Step 2 Unscrew silver screws on both sides...

Page 16: ...2021 SPT Labtech Ltd 16 Step 3 Raise the hood pull out the support rod to place the hood...

Page 17: ...2021 SPT Labtech Ltd 17 Step 4 Adjust the plunger position by using the software Step 5 Exchange the core and select OK and Apply to return to the main menu...

Page 18: ...The function operation menus will vary depending on the type of plate full head or single column head and channel tip the user selects For example here are the di erent types of menu for Aspirate fun...

Page 19: ...nel 4 Ch O et 384 Channel 96 Ch O et 16 Channel 8 Ch O et Aspirate Dispense Empty Mix Transfer Serial Dilution Fill Plate Pooling 96 Core 384 Core 96 Channel 24 Ch O et 8 Channel 4 Ch O et 384 Channel...

Page 20: ...e manufactured instrument to help you get the best results o It is highly recommended that you use disposable tips manufactured by SPT Apricot Designs because they are designed to perform optimally wi...

Page 21: ...2021 SPT Labtech Ltd 21 Here s a general overview of how a user will run the apricot S3...

Page 22: ...p a Username and Password for you If you have any questions please contact SPT Labtech for assistance The default language is English Chinese is also available Unless changes are made in the next step...

Page 23: ...Admin 9663299 Medium Super User Apricot 654321 Low User Customer 123456 Functions Admin Super User User Account Change Core Change Password Create Log File Clear Plunger Con uration Clean Log File Re...

Page 24: ...SPT Labtech Ltd 24 If the password is entered incorrectly an alert message will pop up for 10 seconds Make sure to input the correct password within 10 tries Otherwise the login will be locked for 5 m...

Page 25: ...Password from Setting Fill in Username and Password Then click Add Super User on the left To delete the Super User account select the desired username and click Delete User Admin can restore a previou...

Page 26: ...ername for Super User account As shown in the picture below it does not have the same access as the Admin account It does not have the option to Enable GLP Clear Plunger or Reset the instrument Fill i...

Page 27: ...User The username for general User account is Customer It is the most basic account The purpose for User account is to open the le and run the protocol Therefore it cannot create new les edit labware...

Page 28: ...2021 SPT Labtech Ltd 28...

Page 29: ...nnot edit most of the features in settings Users can only access Change Core and Change Password Click Change Password then select the desired Username and ll in the current and new password elds Con...

Page 30: ...built in USB wired connection or the Bluetooth connection There are 2 steps for setting the connection up hardware and software Both need to be satis ed to utilize the instrument either by Wire conne...

Page 31: ...2021 SPT Labtech Ltd 31 2 Raise the hood 3 Adjust the left switch to either Wire or BLT Bluetooth Adjust the right switch to Run...

Page 32: ...e Connection check the COM Port setting in the computer device manager Con rm the port number in the drop down matches the port number in the device manager For Bluetooth connection input the Bluetoot...

Page 33: ...alization Method 1 After open the software a con rmation window will be popped up asking the user to initialize the pipettor Click on Yes will initialize the instrument Method 2 Another way to initial...

Page 34: ...1 This method is usually for debug purpose only 1 Push the STOP button to in position while the instrument is powered o 2 Turn on the Power 3 Release the STOP button for the instrument to initialize N...

Page 35: ...etup tab There are 2 sections Select Head and Select Plate User can browse all the heads and select the desired one Or choose the channel rst then select the desired volume If there s a protocol creat...

Page 36: ...sired station to put If user wants to create a new plate then leave the station empty and proceed to calibrate plate To copy plate select the desired plate input a di rent plate name and select Copy P...

Page 37: ...td 37 There s an option to select My Plate Only under Select Plate section By checking the box software will only display the plates that created by that speci c account Unchecking My Plate Only will...

Page 38: ...nder My Plate o Cannot rename or delete another Super User s plate or Admin s plate o Cannot calibrate another Super User s plate or Admin s plate Although it can go through the steps for plate calibr...

Page 39: ...o train plate To calibrate new plate click Training Plate from Setup tab then select the empty station Follow the steps to de ne the plate name and de ne the plate type Note that Step 1 and Step 2 are...

Page 40: ...ull 24 head can train 24 well plate and 96 well plate but it cannot train 384 Well For more detail please refer to the table in Section 3 8 After de ning the type of the plate user can follow each ste...

Page 41: ...late click Calibrate Plate from Setup Main Menu then select the desired plate to enter Calibrate Plate menu Follow the steps to calibrate the plate Notice that Step 1 and Step 2 are not allowed to mak...

Page 42: ...xisting protocol opened select New File once will empty the Protocol Line Section Select the second time will empty the Plate Display When having an empty protocol and existing Plate Display select Ne...

Page 43: ...e a e to a di erent folder o Select the le user wants to move o 2 methods to move o Drag the le to the desired folder o Double click the folder To create a new folder o Enter a new folder name o Selec...

Page 44: ...Name and select Apply to save This function can be used to de ne the head type and plate type in each station It can also rename copy and delete plate It is covered in Section 5 2 2 This function is u...

Page 45: ...being put on the S3 This will show di rent numbers of stations depending on di rent machine model User can use the functions in this section to create protocol Detail will be discussed in Section 6 Th...

Page 46: ...can create or edit protocol during Simulation Mode but have to switch to Operation Mode to run the protocol Software will automatically detect which core user put in after initialization Select Head...

Page 47: ...right Then operate using the manual operations commands on the left To perform any operation in Manual mode the procedure would be 1 De e the head type and plate type 2 Select the station number to pe...

Page 48: ...there are liquid inside the tips select once will empty the liquid Select the second time will blow the excessive air out of the tip If there are no liquid inside the tips select once will perform Bl...

Page 49: ...system is connected head and plates are de ed and the plates are trained user can start to create protocol Procedure to operate the functions would be 1 Select the desired function 2 Select the desire...

Page 50: ...ith 384 well plate The options for user are to edit the station number aspirate volume pause period aspirate depth and speed pre and post airgap increased steps Preview Position and quadrant selection...

Page 51: ...to the volume to open Change Value Interface shown below User can either use o The sliders on the left o The number pad on the right o Click Select Variable in the bottom to set the volume variable I...

Page 52: ...airgaps will be emptied after other dispensing functions Increased Steps can be edit between 1 to 10 It is the number of di erent aspiration points from Start Depth to End Depth After user changes the...

Page 53: ...on that will be operated by the instrument In this example it is using a 96 core with 96 channel head and aspirate in a 96 well plate all wells are highlighted Below shows the Position Preview for a 9...

Page 54: ...ll dispense total of 20uL There are two dispensing speeds for dispensing One is for the volume over 60uL and the other one is for the volume less than 60uL User can only select one of the sides under...

Page 55: ...om Touch Depth after liquid dispensed Decremental Steps can be edit between 1 to 10 It is the number of di rent dispensing points from Start Depth to End Depth After user changes the Decremental Steps...

Page 56: ...Labtech Ltd 56 Below shows the Empty menu for 96 Channel tip with 384 Well plate Most of the features are already covered in previous sections Notice that for Empty function only one quadrant can be...

Page 57: ...his feature can be set as variable After enabling this feature the tip will mix by each side depending on the mix cycles The order the tip will be mixing on the side is left front right back For examp...

Page 58: ...e that the Quadrant gure only showed under Source because station 1 is a 384 well plate Select Source Column will open a visualized 384 wells which allows user to select the desired locations to perfo...

Page 59: ...2021 SPT Labtech Ltd 59...

Page 60: ...epresents the moving direction in Preview Position The interval is for the columns that will be perform Serial Dilution between Start and End Column If using the default 1 interval the software will r...

Page 61: ...elow shows the Operate Position Preview from the Serial Dilution Menu The instrument picks up 10uL from the Source Station 2 Column 1 Then mix and dispense 10uL in the Target Station 1 from Column 1 t...

Page 62: ...umns at once it will go back and forth from the Source well to the Target well until the lling is complete Below shows the Fill Plate menu for 8 Channel tip from 384 Well plate to 96 Well plate This f...

Page 63: ...ech Ltd 63 Below shows the Operate Position Preview for Fill Plate The instrument will aspirate 60uL from Source Station 1 and dispense 10uL to the Target Station 2 from Column 12 to Column 1 with an...

Page 64: ...en the Source and Target plate In the example showed above the instrument will o Aspirate 100 L each well from Column 1 to 5 in Source Station 2 o Dispense all 500 L to Column 10 in Target Station 1 o...

Page 65: ...th Mixing but the purpose is to wash the tips with or without Pump Above shows the example to wash 96 channel tip in a 384 plate Notice the quadrant ure is not showing for user to select because syste...

Page 66: ...Labtech Ltd 66 If the instrument connects to the Pump select Yes to use pump This is the period in seconds the system can pause before washing This is the period in seconds the system can pause after...

Page 67: ...eriod by using Minute and Second 2 Enable Wait for User 3 The rst method will disable Comment and Change Tip features The second method will disable Minute and Second features Comment will be shown if...

Page 68: ...2021 SPT Labtech Ltd 68 This is di rent than the Comment in Pause Comment function is worked as a note that can be seen in protocol lines for user and will be ignored during the Protocol running...

Page 69: ...t and 10 is the fastest Dwell Time is the pausing time after aspirating or dispensing in the liquid Overshoot is the liquid volume the system will aspirate before the aspirate volume and dispense afte...

Page 70: ...e Loop Cycle number will be decreasing simultaneously until Protocol nished Inner Loop user can select the function Start Loop to edit the Loop Name and Loop Cycle The protocol lines between Start Loo...

Page 71: ...2021 SPT Labtech Ltd 71...

Page 72: ...1 SPT Labtech Ltd 72 Here are some of the detail about the features in Settings Menu Enabling this function will prompt the user to con rm the maximum volume of the selected head after each initializa...

Page 73: ...rface and will be able to edit the text le Uncheck Enable GLP will be allowed to edit text le Text les can be found in C Program Files x86 SPT Labtech S3 ProtocolTXT Uncheck and check Enable GLP will...

Page 74: ...n Here s a brief view of what are the number representing for aspirate To edit the volume side aspirate speed and station number use must edit all related parameters Here s an example to edit the aspi...

Page 75: ...2021 SPT Labtech Ltd 75 Save the Notepad Then re open the le using Open File under the Protocol tab The preview and the actual aspirate volume will be changed...

Page 76: ...21 SPT Labtech Ltd 76 Enabling this function will allow user to select liquid type The left gure below shows enable CVTC The right gure below shows disable CVTC Refer to 3 7 Accessing the Pipetting Co...

Page 77: ...Edit the parameters from Con guration to change the system default settings Please consult an SPT representative for any questions about the parameters Changing parameters without fully understand may...

Page 78: ...just above the bottom of the well not touching the bottom surface To set this the user can drive the tip down using small increments until it touches the plate Then move up in small increments until t...

Page 79: ...r an accurate initial dispense The following are standard Overshoot volumes for the SPT Labtech Apricot pipettors based on the high volume of the system o 125 L 3 L Overshoot o 550 L 12 L Overshoot o...

Page 80: ...pense hanging drops thus providing a complete dispense It is very useful for dispensing into a dry plate Slowing the plunger speed can improve results when working with viscous materials The slower sp...

Page 81: ...instrument will stop and the Z elevator will move down in case something is pinched A message will appear on the screen instructing the user to put a reservoir under the tips to catch any remaining li...

Page 82: ...Using a clean cloth moistened with ethyl alcohol or cleanser wipe down the instrument surfaces Avoid cleaning the Touch Screen with solvent or cleanser that may damage the screen surface Regularly in...

Page 83: ...2021 SPT Labtech Ltd 83 Solution Right click the icon to open Properties go to Compatibility tab and check Run the program as an administrator then select OK to exit Program should be able to open now...

Page 84: ...arefolder Usually under C Program Files SPT Labtech 2 Right Click the Folder and select Properties 3 Select Security Then click Edit 4 Check Allow full control for the Users 5 Click Apply thenOK to cl...

Page 85: ...ess SPT Labtech products are renowned for their industry leading reliability and e ciency With our reliance service you have access to a dedicated support team to safeguard your investment and secure...

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