Slee Cryostat MEV Operating Instructions Manual Download Page 1

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Instructions – MEV

OPERATING
INSTRUCTIONS

INS2000GB
2011-12-11

INSTRUCTIONS

Cryostat

MEV

Summary of Contents for Cryostat MEV

Page 1: ...Instructions MEV OPERATING INSTRUCTIONS INS2000GB 2011 12 11 INSTRUCTIONS Cryostat MEV 1 ...

Page 2: ...Instructions MEV 2 ...

Page 3: ...SAFETY NOTES 4 4 COMPONENTS 8 5 SPECIFICATIONS 9 6 UNPACKING AND INSTALLATION 10 7 INITIAL OPERATION 13 8 OPERATION OF MICROTOME 19 9 OPERATION OF CRYOSTAT 28 10 CLEANING AND MAINTENANCE 32 11 SERVICE 34 12 OPTIONAL ACCESSORIES 35 13 WARRANTY 36 14 DISPOSAL 36 ...

Page 4: ... during the upstroke of the specimen which avoids rubbing on the disposable blades or microtome knives This stops rapid deterioration of the disposable blade or microtome knife 2 SYMBOLS Dangers warnings and cautions are marked by this symbol i Special instructions regarding the operation of the instrument are marked by this symbol 3 SAFETY NOTES The SLEE Medical open top cryostat MEV is provided ...

Page 5: ...n before working with the knife or specimen before changing the specimen during break time Hand wheel stop released Movement Hand wheel stop activated No movement The hand wheel can be stopped in any position For this purpose move the stop lever towards the centre For releasing the stop please turn the lever towards the outside again ...

Page 6: ...MEV 6 3 2 FINGER PROTECTION Use the finger protection always Before you start any work with the knife or specimen Before changing the specimen During work break Finger protection released Finger protection activated ...

Page 7: ...SPOSABLE BLADES Microtome knives and disposable blades have extremely sharp edges and this can lead to injuries Please be extremely careful when handling microtome knives and disposable blades Do not place microtome knives or disposable blades at unsecured areas Never position microtome knives or disposable blades with the sharp edge pointing upwards Store blades in a covered container Use a conta...

Page 8: ... unit with refrigeration system Semi automatic rotary microtome Operation manual 2x Specimen holder 1x Ø 22mm 1x Ø 35 mm clear cut Brush Anti roll plate Ready to use 1x Bottle cryostat low temperature oil 4x 100 ml CryoGlue embedding medium 1 Box of disposable blades 50 pcs or 1 steel knife 16 cm C profile Set of Allen keys ...

Page 9: ...n 2 µm steps 50 100 µm in 5 µm steps Horizontal specimen advance 28 mm Vertical specimen stroke 57 mm Specimen retraction on return stroke 0 200 µm selectable Dimensions and weight width x depth x height 600 mm x 680 mm x 1130 mm Weight without accessories 110 kg Specimen orientation horizontal 8 Specimen orientation vertical 8 Specimen orientation z axis 360 Trimming thickness 0 300 µm freely pro...

Page 10: ...it see section simple service work and lift the unit slightly with one hand at the outer edge and the other hand at the rear edge of the cover move approx halfway off the pallet and lower the front to the ground After this the unit is lifted at the rear the pallet is removed and the rear part lowered to the ground Option 2 If the unit has to be transported further the wheelbarrow is useful Push th...

Page 11: ...d rear of the instrument of 10 20 cm should be guaranteed A free ventilation of fresh air from underneath the instrument should be guaranteed 6 2 INSTALLATION For opening the chamber push the window to the top Remove the transport security underneath the specimen holder and position the waste tray After installation wait at least 4 hours before switching the instrument on Failure to comply may res...

Page 12: ...wheel lever that is folded away for transport reasons Before you start any work unfold the hand wheel lever by simply pulling the lever into a horizontal position For folding the lever back pull the horizontally orientated lever and fold it away Hand wheel lever in folded position Hand wheel lever unfolded ...

Page 13: ...ure the target chamber temperature the status of the quick freeze unit optional and the total electronic status of the instrument and a cutting counter optional TUE 13 05 05 CUT 7 µm Trim 19µm Cha 20 C 22 QF 0 Status OK 0000 7 2 CONTROL PANEL Software settings and motor operation can be operated with the control panel on the left to side of the instrument MEV Control Panel ...

Page 14: ...rast TIME TUE 13 05 05 To move further up in the main menu list Press Light Auto Light duration 10 m Chamber incr 15 C Retraction 5 µm To change values select a setting Press to open the setting Change entries by pressing or Store new settings by pressing Light duration 10 m Chamber incr 15 C Retraction 5 µm Start defrost Press to return to the main menu TUE 13 05 05 CUT 7 µm Trim 19µm Cha 22 C 25...

Page 15: ...dministrators Enter PIN 0000 Select defrosting programs 1 to 3 and set a time for the automatic defrosting programme e g each day at 12 00 h and at 23 00 h in this example Defr Start 1 12 00 Defr Start 2 23 00 Defr Start 3 __ __ Disinf Req __ __ Select temperature increase start and set a starting time for the automatic power saving mode e g start at 21 00 h in this example Disinf delay 10 h Incr ...

Page 16: ...rst select a time when the system will ask for a disinfection cycle e g at 12 00 h the instrument will ask for a disinfection cycle Disinfec Req 12 00 Disin Days 12____ Disin Delay 10 h Select the days in which a disinfection cycle can be activated e g Monday and Tuesday in this example Disinfec Req 12 00 Disin Days 12____ Disin Delay 10 h 1 Monday 2 Tuesday 3 Wednesday 4 Thursday 5 Friday 6 Satur...

Page 17: ...ith the optional fast cooling peltier system its function can be activated or deactivated The default setting for automatic deactivation of the quick freeze is set to 30 minutes The timer for automatic deactivation can be set by an authorized service technician UV disinfection optional ON OFF If the instrument is equipped with the optional UV C disinfection system its function can be activated or ...

Page 18: ...ivated Trim latching ON OFF If Trim latching mode is activated trimming function is activated and deactivated by pressing once If Trim latching mode is deactivated trimming function is activated by continuously pressing Contrast Display contrast Time Day and Time Language A language can be selected out of a list of available languages Display version Display of software version Program settings Up...

Page 19: ...the knife holder counter clockwise Remove Insert knife Adjust knife height by turning the wheels on the left and right side of the knife holder base Tighten the knife fixation by turning the two screws in the front of the knife holder clockwise Release hand wheel lock for cutting Loosening of knife fixation and insertion of new knife Fixation and height adjustment of knife ...

Page 20: ...and wheel lock Remove finger protection Loosen the blade fixation by turning the right lever counter clockwise Remove Insert blade from one side Tighten the blade fixation by turning the right lever clockwise Loosening of blade fixation and insertion of new blade Fixation of blade ...

Page 21: ...lade holder A tilt towards the object holder increases the cutting angle clockwise turn of the knob a tilt away from the object holder decreases the cutting angle counter clockwise turn of the knob Tighten the blade holder fixation by turning the right lever on the blade holder base clockwise Release hand wheel lock for cutting Loosening of blade holder fixation and adjusting new cutting angle inc...

Page 22: ...dding medium e g GSV1 on the holder and carefully press the object on top Wait until the object is frozen mat surface or pressure 8 4 INSERTION OF OBJECT HOLDER Turn hand wheel to its highest position and activate the hand wheel stop Release the object holder fixation by turning the fixation lever counter clockwise Remove Insert object holder Tighten the object holder by turning the fixation lever...

Page 23: ...For upward and downward orientation use the upper orientation wheel For orientation to the left and right use the left orientation wheel Close the orientation fixing lever Release hand wheel lock for cutting Opening of orientation fixation lever and orientation of object holder upwards and downwards Orientation of object holder to the left and right Fastening of orientation fixation ...

Page 24: ...To change the cutting thickness press or to increase or decrease the value 8 10 TRIMMING OF SPECIMEN Insert object holder Orientate specimen as desired Deactivate the hand wheel stop Release finger protection If Trim latching mode is activated trimming function is activated and deactivated by pressing once Activation of trimming function is displayed as illustrated TUE 13 05 05 TRIM Trim 19µm Cha ...

Page 25: ... in the display e g 7 µm in this example TUE 13 05 05 CUT 7 µm Trim 19µm Cha 20 C 22 QF 0 Status OK 0000 Turn hand wheel until some even sections have been obtained Clean the microtome knife or disposable blade always away from the cutting edge with a cold brush Position anti roll plate onto the microtome knife or disposable blade and cut If necessary readjust the height of the guide plate Leave h...

Page 26: ...the height of the anti roll plate turn the wheel to the left or right For pick up of the samples turn the anti roll plate side wards with the help of the knob Height adjustment of anti roll plate i For height adjustment of the anti roll plate it is recommended to adjust the correct height during cutting ...

Page 27: ...ain 7 to 10 Breast with fat 25 to 30 i For tissue which has to be cut considerably below normal chamber temperature e g fatty tissues and in order to avoid cooling artefacts an additional bench top CO2 freezer is available Exceptionally freezing spray can be used Breast without fat 16 to 20 Cartilage 13 to 20 Fat 30 to 40 Heart 20 to 25 Intestinal 13 to 20 Kidney 13 to 20 Larynx 13 to 16 Lip 10 to...

Page 28: ... light will be deactivated upon opening of the lid of the cryochamber The UVC disinfection will be aborted and has to be activated again for surface disinfection The default setting for automatic deactivation is set to 30 minutes The timer can be set by an authorized service technician 9 2 QUICK FREEZE POSITION only applicable to instruments equipped with this feature For convenience of the user t...

Page 29: ...equipped with an heat extractor Apply the heat extractor on top of the specimen during the freezing process Heat extractor without quick freeze positions Heat extractor With quick freeze positions i Use of the heat extractor can lead to changed orientation of the specimen in the frozen block especially for smaller samples If precise orientation of the specimen is required the freezing procedure sh...

Page 30: ...tic defrost or an manual activated defrost hot gas from the cooling system is pressed through the evaporator All ice which has built up is melted the water drops into a heated tray below of the evaporator and from there it flows into a tray with hot tubes and evaporates inside the instrument The defrost will last till the system reaches a temperature of 20 C on the evaporator The temperature insid...

Page 31: ...ochamber Switch off the instrument Place a container at the flexible outlet hose to collect all the melt water Remove the rubber plug at the right backward of the cryochamber Dry the inside of the cryochamber completely Switch on the instrument Flexible hose to drain out melt water Removal of rubber plug in cryochamber i Before starting a complete defrost of the instrument assure that all samples ...

Page 32: ...out removing the microtome from the cryochamber When disinfecting the instrument take appropriate protective measures gloves mask protective clothing etc Wear protective clothing and disposable gloves according to Good Laboratory Practices When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer The integrated anti roll plate of the blade h...

Page 33: ...ed by authorized SLEE service technician check of all functions complete defrost removing of microtome oiling of movable parts check of cooling system check of driving system complete disinfection cleaning drying 10 4 CLEANING OF CRYOSTAT VENTILATION During usage of the instrument dust will deposit on the ventilation of the cooling unit This can negatively affect the cooling performance of the ins...

Page 34: ...able Complete contact details Type of instrument and serial number Place of instrument and name of user Purpose of service call Delivery date of the unit If it is necessary to return the instrument it must be cleaned and disinfected before delivery It must be returned in its original packing If the instrument or parts thereof are sent back in a dirty or non disinfected condition SLEE reserves the ...

Page 35: ... holder Ø 22 mm 30000002 Object holder Ø 35 mm 30000000 Object holder Ø 50 mm 30000003 Cork plate 100 pcs Ø 20 mm 30001001 CryoGlue embedding medium 4x 125 ml 30001100 clear cut Brush 30001030 Anti roll plate disposable blade holder 32001050 Anti roll plate standard knife holder 32001049 Disposable blade low profile 50 pcs 28407000 Disposable blade high profile 50 pcs 28408000 Freezing spray 173 g...

Page 36: ... Unauthorized modification or repair by third party persons will void the warranty Only original SLEE spare parts must be used Guarantee claims can be put forward only if the instrument is used according to this manual and for the purpose described Mistakes and errors which occur because of improper use cannot be accepted 14 DISPOSAL The instrument or parts of the instrument must be disposed of ac...

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