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Sigma 3-16P, Operating Manual

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Sigma 3-16P Operating Manual Download Page 13

 

Version 06/2007, Rev. 1.15 of 16/09/2011 - sb 

 

  page 13 of 75 

Translation of the original operating manual 

 

1.4 

Scope of Supply 

 
The centrifuge comprises: 
 

•

 

1 connecting cable 

depending on the voltage variant 

•

 

1 rotor wrench 

Part no. 930 100 

•

 

20 ml slushing oil 

Part no.   70 104 

•

 

1 tube of grease for load-bearing bolts 

Part no.   70 284 

 
 
 
Documentation: 
 

•

 

Operating manual incl. EU Declaration of Conformity (page 5) 

 
 
 
 
Accessories 
 
according to your order, our order confirmation, and our delivery note. 
 
 
 
 
 

1.5 

Standards and Regulations 

 
Please refer to the enclosed EU Declaration of Conformity (page 5). 

 

Summary of Contents for 3-16P

Page 1: ...Version 06 2007 Rev 1 15 1 of 05 10 2011 sb Translation of the original operating manual Operating Manual Centrifuge 3 16P Please retain for later use...

Page 2: ......

Page 3: ...ght by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Phone 49 0 55 22 50 07 0 Fax 49 0 55 22 50 07 12 E mail info sigma zentrifugen de For service please use our se...

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Page 7: ...Operating Manual 15 2 3 Informal Notes on Safety 15 2 4 Safety Instructions for Centrifugation 16 2 4 1 Special Instructions 17 2 4 2 Resistance of Plastics 18 2 5 Prohibited Centrifuging Operations...

Page 8: ...l Start Up 28 5 2 1 Switching the Centrifuge ON 28 5 2 2 Opening and Closing the Lid 28 5 2 3 Installation of Rotors 29 5 2 3 1 Installation of Angle Rotors with a Hermetically Sealed Lid30 5 2 4 Inst...

Page 9: ...on the Display 43 6 2 2 Centrifuge cannot be started 43 6 2 3 Centrifuge decelerates during Operation 43 6 2 4 Lid cannot be opened 44 6 2 5 Emergency Lid Release 44 6 2 6 Error Codes 45 6 2 7 Service...

Page 10: ...ies 55 10 1 Maximum speed for tubes 65 10 2 Graphical Representation of the Rotors 66 11 Appendix 67 11 1 Formulae Mathematical Relations 67 11 1 1 Relative Centrifugal Force RCF 67 11 1 2 Density 67...

Page 11: ...ved by all persons operating the centrifuge In addition the local rules and regulations for the prevention of accidents must be complied with 1 2 Intended Use Centrifuges are power driven machines tha...

Page 12: ...energy Nm 14 500 1 000 21 475 9 530 Other parameters Time range Storage locations 10 sec 11 h 59 min short run continuous run 50 Physical data Depth mm Width mm Height mm Weight kg EMC as per EN 61326...

Page 13: ...oltage variant 1 rotor wrench Part no 930 100 20 ml slushing oil Part no 70 104 1 tube of grease for load bearing bolts Part no 70 284 Documentation Operating manual incl EU Declaration of Conformity...

Page 14: ...e d emploi Ein Netzverbindung On Power Marche mise sous tension Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Te...

Page 15: ...tr s importante Fig 2 2 Symbols used in the Operating Manual 2 3 Informal Notes on Safety The operating manual is an integral part of the product Please retain the operating manual throughout the serv...

Page 16: ...been approved by the manufacturer In case of doubt contact our service team See 6 2 7 Service Contact page 46 Ensure that rotor and buckets are correctly fitted see 5 2 3 Installation of Rotors page 2...

Page 17: ...on of infectious toxic radioactive or pathogenic substances Spin infectious material in sealed rotors and buckets only in order to prevent the material from leaking into the centrifuge Avoid the corro...

Page 18: ...he following hazard warnings In the case of non compliance with the instructions the manufacturer cannot be held liable or subject to any warranty claims Only persons who have read and understood the...

Page 19: ...e if necessary Risk of crushing Attention Ensure that all repairs are performed only by authorized and specialized personnel see 6 2 7 Service Contact page 46 2 5 1 Special Hazards Do not open the lid...

Page 20: ...parts must be replaced immediately Fastening of the trunnion pins in the rotor Screw connections Rotors and accessories see 5 2 5 Service Life of Rotors an Accessories page 32 2 7 Instructions for Eme...

Page 21: ...ntrifuge always reach under the centrifuge from the side Install the transport safety device foamed plastic ring into the rotor chamber Transport the centrifuge within suitable packaging and if at all...

Page 22: ...the centrifuge from the side Attention The centrifuge weighs approx 48 kg Please retain the packaging for any possible future transport of the centrifuge 4 1 1 Transport Safety Device The transport s...

Page 23: ...operating voltage on the name plate must correspond to the local supply voltage SIGMA laboratory centrifuges are units of safety class I and have a three wire power cord with a IEC C13 connector They...

Page 24: ...fuge 5 1 Description 5 1 1 Operating Elements 1 Mains power switch 2 Stop key 3 Lid key 4 Display 5 Lid 6 Start key 7 Left rotary knob 8 Right rotary knob 11 Fig 5 2 Rear view of the centrifuge Fig 5...

Page 25: ...e 1 Manufacturer and registered office 2 Type name 3 Serial number 4 Max speed 5 Kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for special disposal see chapter 8 10 CE mark in a...

Page 26: ...5 1 4 Operation and Display The display is a hermetically sealed LCD display It is operated by way of two rotary knobs and indicates the operating statuses 5 1 5 Electronic System The microprocessor c...

Page 27: ...al system check monitors the data transfer and sensor signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages with a number on the speed and rc...

Page 28: ...y then illuminates 5 2 2 Opening and Closing the Lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid see 5 3 7 A...

Page 29: ...e turns and fastened again This must be done once a day or after 20 cycles This ensures a proper connection between the rotor and the motor shaft The rotor tie down screw should only be tightened when...

Page 30: ...shaft Tighten the rotor tie down screw with 7 5 Nm using the supplied rotor wrench so that the spring washer assembly is compressed tightly The rotor can also be used without a cover Slightly grease t...

Page 31: ...ed The tubes must be installed symmetrically so that the buckets and their suspensions are loaded evenly Centrifugation with different tube sizes Working with different tube sizes is possible In this...

Page 32: ...sive wear of the bearings In high speed angle rotors the vessels must be filled up to their useful volume the volume stated for the vessel If the vessels are only partially filled they will deform Thi...

Page 33: ...raving on the bucket rotor Attention Refer to the table of rotors and accessories with a different service life see chapter Fehler Verweisquelle konnte nicht gefunden werden Appendix 5 2 6 Starting th...

Page 34: ...softstop function Press the key three times to active the softstop function alone Press the key four times to activate the softstart function and the brakeless deceleration The soft stop display flas...

Page 35: ...he input If this is not done the value will be automatically reset to the last setting After 11 59 min the display changes from min sec to hrs min The time can then be changed in steps of 10 minutes I...

Page 36: ...he duration of the short run is displayed Fig 5 12 When the start key is released the centrifuge decelerates at maximum power to a standstill the start key is illuminated After finishing the short run...

Page 37: ...or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired value is displayed Press the right rotary knob or the start key to confirm the selected value If this i...

Page 38: ...t appears in front of the parameter rcf on the display Fig 5 15 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired value is...

Page 39: ...l set appears in front of the option Rotor on the display Fig 5 16 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired rotor...

Page 40: ...y knob until the desired program number is displayed Empty storage location numbers flash Press the right rotary knob or the start key to confirm the input The settings are now saved under this progra...

Page 41: ...function the padlock symbol Fig 5 19 will flash Now the centrifuge can be started and stopped but the settings cannot be changed Proceed in the same way in order to deactivate the function The current...

Page 42: ...signal can be heard at the end of the operation as well as in the event of an error message or imbalance Press the lid key five times and on the fifth time hold it down for approx two seconds Proceed...

Page 43: ...ly Power cord plugged in and line voltage present Plug in power cord correctly Fuse OK Switch unit off and let fuses cool down for approx 2 min Mains power switch on Switch on power Lid closed Close l...

Page 44: ...the lid seal Apply talcum powder to the seal to avoid sticking 6 2 5 Emergency Lid Release In the event of a power supply failure it is possible to manually open the centrifuge lid using a stopper It...

Page 45: ...efrigerated centrifuges allow to slow down power off on allow to cool down provide better ventilation 46 49 Imbalance error allow to slow down power off eliminate the imbalance 50 59 Lid error press l...

Page 46: ...rifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 84 25 Fax 49 0 55 22 50 07 94 25 E mail service sigma zentrifugen de outside Germany contact our agency in your country...

Page 47: ...entrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 7 1 1 Centrifuge Disconnect the power cord from the socket before cleaning Carefully remove all liquids includin...

Page 48: ...d with toxic radioactive or pathogenic substances clean them immediately with a suitable decontamination agent depending on the type of contamination Take suitable precautions for your own safety if t...

Page 49: ...t least once a month for cracks visible damage of the surface pressure marks signs of corrosion other changes Check the bores of the rotors and multiple carriers Replace any damaged components immedia...

Page 50: ...from the rotor chamber Grease the upper third of the centrifuge chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2 000 rpm The glass and metal particle...

Page 51: ...e min max time min max cycles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30 40 20 Polypropylene tubes 115 118 30 40 30 Teflon tubes 134 138 3 5 100 Aluminum rotors 134 138 3 5 Polycarbonate...

Page 52: ...his is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dismantled state Rubber parts shou...

Page 53: ...fix the original form in a clearly visible manner to the outside of the packaging Attention If no such declaration is enclosed in the return package we will perform the decontamination at your expense...

Page 54: ...ll in the enclosed declaration of decontamination Please comply with any other applicable local rules and regulations 8 2 Disposal of the Packaging Use the packaging to return the centrifuge for dispo...

Page 55: ...18000 18200 sealable with cap no 17112 max tube length 115 mm 1 set 2 pcs max radius 17 1 cm min radius 7 6 cm 4 200 3 372 17112 Rectangular polysulfone sealing cap clear incl 1 set clips no 17118 1 s...

Page 56: ...ture tubes 50 ml Nunc Corning Falcon Greiner e g 15151 polypropylene 18053 Rectangular carrier for 2 culture tubes 50 ml with skirt Nunc Corning Falcon Greiner max 29 5 38 x 85 118 mm polypropylene 18...

Page 57: ...0 mm e g no 15060 RIA tubes hemolyse tubes 1 set 2 pcs 17353 Round carrier for 20 reaction vials 1 5 2 0 ml max 11 mm e g no 15008 15040 1 set 2 pcs 17354 Round carrier for 16 round bottom tubes 7 ml...

Page 58: ...0 Round carrier for 1 bottle with flat bottom 200 ml max 57 x 90 115 mm e g no 15202 1 set 2 pcs 17347 Round carrier for 1 bottle with flat bottom 250 ml max 61 5 x 90 125 mm e g no 15247 15249 13255...

Page 59: ...ml round and flat bottom max 16 8 x 90 105 mm e g no 15015 15023 15024 1 set 2 pcs 17018 Round carrier for 4 tubes 9 15 ml round and flat bottom max 17 20 x 90 110 mm e g no 15015 15020 15022 15023 1...

Page 60: ...e vessels could be damaged at speeds exceeding 13 000 rpm 13 000 13 226 14000 Casset suitable for 20 reaction vials 0 25 0 4 ml no 15014 1 set 2 pcs 14002 Casset suitable for 10 reaction vials 1 5 2 0...

Page 61: ...rotor 8 x 50 ml for tubes e g no 13055 15051 15052 incl hermetic aluminium lid no 17840 max radius 9 6 cm min radius 3 3 cm angle 25 9 000 8 694 19776 Angle rotor for 6 x 50 ml culture e g no 15151 in...

Page 62: ...0 14000 15042 Reaction vials 0 2 ml 100 pcs per pack suitable for 13021 12115 14021 15060 Polystyrene tube 5 ml RIA 12 x 75 mm suitable for 17006 17356 18005 18009 15000 Polyfluor tube 10 ml 16 1 x 81...

Page 63: ...0 mm graduated 2 up to 100 ml in 2 ml increments suitable for 17100 17101 17390 18100 15125 Polypropylene bottle 125 ml 51 x 99 mm incl screw cap suitable for 17125 17395 18125 15202 Polypropylene Co...

Page 64: ...9 Rubber adapter for special glass tubes no 15015 15022 and 15085 1 set 2 pcs suitable for 12155 12159 16030 Rubber adapter for glass tube no 15025 15033 and 15086 1 set 2 pcs suitable for 12150 12156...

Page 65: ...ropolymer tubes and especially high volume tubes can be used in our rotors buckets and adapters at higher speeds than their breaking limit Always fill the tubes up to their useful volume the volume th...

Page 66: ...epresentation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used If necessary the values must be manually calculated see 11 1 1 RCF pa...

Page 67: ...mined by the equation If then the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is altered the speed will be adapted accordingly under the consideration of...

Page 68: ...Version 06 2007 Rev 1 15 of 16 09 2011 sb page 68 of 75 Translation of the original operating manual Fig 11 1 Speed gravitational field diagram...

Page 69: ...4K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 9100 15 000 4 15C 4K15C 4 16 4 16K 6 15 6K15 6 16 6 16K without engravi...

Page 70: ...4 4 1 4 4 2 1 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylate C7H12...

Page 71: ...1 1 1 4 Hydrogen chloride HCl concentrated 1 4 4 4 1 1 2 3 1 4 Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 1 3 Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H2S 10 1 1 1 1 1 1 1 3 1 1 Iodine...

Page 72: ...1 4 Sodium bisulfite NaHSO3 10 1 1 2 4 1 1 1 Sodium carbonate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 4 Sodium hydroxide NaOH 50...

Page 73: ...agents 48 49 Closing the lid 19 29 Condensation 24 Connected load 12 Connecting cable 13 Contamination 19 48 49 Continuous heat resistance 51 Continuous run 37 Corrosion 17 19 20 33 48 49 50 51 Cost e...

Page 74: ...iple carrier 33 50 Noise level 12 Online download of forms 54 Opening the lid 29 Operating voltage 24 Operational safety 19 49 Overseas shipping 22 Padlock symbol 42 Parameter 12 27 38 39 42 46 68 Pat...

Page 75: ...vice request 3 47 53 Service work 53 Slushing oil 13 49 50 Solvents 18 48 49 Sound signal 28 43 44 Specialist personnel 53 Storage temperature 12 22 Stress corrosion 50 Structural changes 20 Supply vo...

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