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Sigma 3-16L, Operating Manual

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Sigma 3-16L Operating Manual Download Page 1

Translation of the original operating manual 

sb 
03/2014 
Rev. 2.11 of 11/08/2017 
Sigma 3-16L;Laboratory Centrifuge;Sigma 3-16L IVD 

Laboratory Centrifuge 

part no. 0700902 

 

Sigma 3-16L 

 

Sigma 3-16L IVD 

 

 

 

from serial no. 146284 

 

 

Laboratory Centrifuge 

Operating Manual 

 

 

Please retain for later use! 

 

 

Summary of Contents for 3-16L

Page 1: ...al sb 03 2014 Rev 2 11 of 11 08 2017 Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD Laboratory Centrifuge part no 0700902 Sigma 3 16L Sigma 3 16L IVD from serial no 146284 Laboratory Centrifuge Ope...

Page 2: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 2 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902...

Page 3: ...of the original operating manual part no 0700902 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 3752...

Page 4: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 4 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902...

Page 5: ...us and relative centrifugal force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel...

Page 6: ...allation of the rotor 28 6 2 2 2 Installation of angle rotors with a hermetically sealed lid 28 6 2 2 3 Installation of accessories 29 6 2 2 4 Adapters 30 6 2 2 5 Tubes 30 6 3 Control system Spincontr...

Page 7: ...bearing bolts 49 8 1 5 Glass breakage 50 8 2 Sterilisation and disinfection of the rotor chamber and accessories 50 8 2 1 Autoclaving 51 8 3 Service 52 8 4 Return of defective parts 53 9 Disposal 55...

Page 8: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD Table of contents 8 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902...

Page 9: ...23 2 1 1 2 Intended use The laboratory centrifuge is suitable for the separation of constituents of different densities in mixtures with a maximum density of 1 2 g cm3 The laboratory centrifuge that i...

Page 10: ...r dmodule 010 Allgemei ne Infor mati onen 010 0050 N or men und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC decl...

Page 11: ...020 Aufbau und Wir kungsweise 020 0010 Aufbau der Zentrifuge 26 mod_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 16L 020 Aufbau...

Page 12: ...020 0010 0020 Typenschild 3 16L 29 mod_1405321590259_68 docx 197155 3 1 2 1 2 Name plate 1 Manufacturer and registered office 2 Type 3 Serial number 4 Max density 5 Nominal voltage 6 Input fuse 7 Symb...

Page 13: ...uadratically as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the...

Page 14: ...e centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagra...

Page 15: ...2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name plate s...

Page 16: ...s In this operating manual the following names and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols...

Page 17: ...Pos 38 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 39 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0041 Bedienpersonal IVD 26 mod_1405319018891_68 d...

Page 18: ...ar dmodule 030 Sicher heit 030 0060 0020 M echanische Sicher heit au er 1 16 1 16K 26 mod_1405319024314_68 docx 192059 3 1 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifu...

Page 19: ...0 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 mod_1405319028141_68 docx 192101 3 1 3 6 4 Chemical and biological safety If pathogenic...

Page 20: ...king glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions o...

Page 21: ...t least once per month for safety reasons Pay special attention to changes such as corrosion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles...

Page 22: ...or signals with regard to plausibility The system continuously performs a self check and identifies malfunctions Error messages are displayed as Error followed by a code number see chapter 7 2 Table o...

Page 23: ...s 74 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisi ken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The unit was built state of the art and acc...

Page 24: ...5319161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature...

Page 25: ...0 BA Zentrifugen Sigma Pr ojekte 3 16L 040 Lagerung und Tr ansport 040 0050 Transportsicherung 3 16L berschrift 29 mod_1405321600880_68 docx 197211 2 1 4 5 Transport safety device Pos 87 100 Sigma 110...

Page 26: ...le Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 93 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss 050 0020 Energieversorgung 27 mod_1405319175630_68 docx 192409 2...

Page 27: ...a Standardmodul e 060 Betrieb_Spi ncontr ol L S 060 0020 Ei nschalten au er 6 16HS 27 mod_1405319201340_68 docx 192451 2 1 6 2 Switching the centrifuge on Press the mains power switch The display then...

Page 28: ...and fastened again This ensures a proper connection between the rotor and the motor shaft CAUTION When using rotors for microtiter plate formats Ensure that the plate holders are inserted together wit...

Page 29: ...eh r 4pl tzig bis 2 16P bis 3 18KS 27 mod_1405319205710_68 docx 192507 4 1 6 2 2 3 Installation of accessories Only use inserts that are suitable for the rotor see chapter 11 1 Range of accessories Al...

Page 30: ...alance If all of the compartments of a carrier are not used the buckets must be loaded evenly Loading the edges of a bucket only is not permissible Pos 114 010 Uni versalmodul e Leerzeile 0 mod_120211...

Page 31: ...berfl che L ohne Temperatur anzeige 27 mod_1405319251981_68 docx 192633 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function kno...

Page 32: ...ened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will b...

Page 33: ...be changed during the centrifugation Fig 11 Setting the speed value or the RCF value Pos 128 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 112 1 Pos 130 010 Uni versalmodul e Leerzeile 0...

Page 34: ...this case the symbol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol F...

Page 35: ...e and final speed are restored and displayed Fig 15 Indication Short during a short run Pos 132 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 133 100 Sig ma 100 BA Zentrifugen S...

Page 36: ...from being exceeded Pos 134 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 135 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Ze...

Page 37: ...ing The bar remains visible as long as the cursor is placed over the symbol Fig 18 The automatic lid opening function Auto Open is activated Pos 138 010 Uni versalmodul e Leerzeile 0 mod_1202116244500...

Page 38: ...ress the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed over the symbol Fig 20 The program lock ProgLock is activated Pos 142 010 Uni ve...

Page 39: ..._1202116244500_0 docx 114 1 Pos 145 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Zent6 060 0030 0020 0141 Eing abesperr e ohne Temp 27 mod_140531930...

Page 40: ...tory Centrifuge Sigma 3 16L IVD 6 Using the centrifuge 40 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902 Pos 146 010 Uni versalmodul e Seiten...

Page 41: ...of an input lock see chapter 6 3 2 13 Input lock Pos 148 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 149 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi n...

Page 42: ...es in the activated status Select the desired program and confirm the selection by pressing the function knob The program is now loaded Fig 25 Loading a program Pos 152 010 Uni versalmodul e Leerzeile...

Page 43: ...d in Plug in power cord correctly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started s...

Page 44: ...fuge lid Switch off the mains power switch and disconnect the power cord from the socket Remove the stopper see figure item 1 from the opening at the side panel e g with a screwdriver Fig 26 Position...

Page 45: ...rror only for refrigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled cent...

Page 46: ...the event of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab...

Page 47: ...thermal stress e g by heat generators Pos 167 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 168 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 080 War tung und Instandhalt...

Page 48: ...d CAUTION Do not clean the accessories in a dishwasher Cleaning in a dishwasher removes the anodised coating the result is cracking in areas that are subject to stress If the rotors or accessories hav...

Page 49: ...of corrosion other changes Check the bores of the rotors and multiple carriers Replace any damaged components immediately for your own safety After every cleaning process grease the rotor tie down scr...

Page 50: ...hamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at t...

Page 51: ...laving Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 11...

Page 52: ...the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension ar...

Page 53: ...ment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and sp...

Page 54: ...port safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in ne...

Page 55: ...f the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapte...

Page 56: ...e I 20 Connected load kVA Power consumption kW Max current consumption A Thermal circuit breaker A 0 70 at 220 240 V 50 60 Hz 0 82 at 120 V 60 Hz 0 85 at 100 V 50 60 Hz 0 50 at 220 240 V 50 60 Hz 0 50...

Page 57: ...of air 80 up to 31 C with a linear decrease to 50 relative humidity of air at 40 C Maximum altitude 2 000 m above sea level Pos 199 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Po...

Page 58: ...ts rectangular carriers no 18015 and 48 PPCO tubes 15 ml no 15023 Radius max 17 1 cm radius min 7 6 cm 4 200 3 372 11182 Swing out rotor 12 x 50 ml culture tubes complete no 11180 incl 2 sets buckets...

Page 59: ...ppendorf reaction caps incl hermetic aluminium lid no 17182 software version 32 or higher Radius max 8 4 cm radius min 4 3 cm 15 000 21 130 12310 Angle rotor for 6 x 50 ml culture tubes Biosafe certif...

Page 60: ...ess steel tube 50 ml 28 5 x 101 5 mm sealable with cap no 17054 suitable for 12150 12156 13082 13059 Adapter for 1 round bottom tube 10 ml max 16 2 19 x 75 85 mm e g no 15000 15010 15039 1 set 2 pcs 1...

Page 61: ...glass tubes the maximum value of 4 000 x g must not be exceeded except special high strength glass tubes please refer to the information provided by the manufacturer Pos 207 010 Uni versalmodul e Lee...

Page 62: ...riginal operating manual part no 0700902 Pos 211 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 mod_1405320705642_68 docx 195153 2 1 11 2...

Page 63: ...mages of the acceleration curves and are assigned the same numbers An exception is curve 0 It decelerates brakeless spin out In general the runtime until the set speed is reached depends on the moment...

Page 64: ...fuge Sigma 3 16L IVD 11 Appendix 64 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902 Fig 31 Diagram of linear curves Pos 214 010 Uni versalmodu...

Page 65: ...rs 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x 1 16 1 16K 12135 5 years 20x 1 16 1 16...

Page 66: ...1 1 4 1 1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4...

Page 67: ...1 2 1 Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimeth...

Page 68: ...100 1 2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1...

Page 69: ...NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sul...

Page 70: ...ion 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902 Pos 219 100 Sig ma 110 BA Zentrifugen Sig ma Proj ekte 3 16L 110 Anhang 110 0060 Konfor mit tser kl r...

Page 71: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 11 Appendix Version 03 2014 Rev 2 11 of 11 08 2017 sb 71 79 Translation of the original operating manual part no 0700902...

Page 72: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 11 Appendix 72 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902...

Page 73: ...Laboratory Centrifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 11 Appendix Version 03 2014 Rev 2 11 of 11 08 2017 sb 73 79 Translation of the original operating manual part no 0700902...

Page 74: ...uge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 11 Appendix 74 79 Version 03 2014 Rev 2 11 of 11 08 2017 sb Translation of the original operating manual part no 0700902 11 7 Declaration of confo...

Page 75: ...rifuge Sigma 3 16L Laboratory Centrifuge Sigma 3 16L IVD 11 Appendix Version 03 2014 Rev 2 11 of 11 08 2017 sb 75 79 Translation of the original operating manual part no 0700902 Ende der Liste f r Tex...

Page 76: ...fuge decelerates during operation 42 Centrifuge is inclined 42 Centrifuge was moved during run 42 Centrifuge cleaning and care 46 Changes in colour 50 Changes in structure 50 Chemical and biological s...

Page 77: ...18 I Imbalance 29 30 48 Imbalance error 44 Imbalance monitoring system 22 Importance of the operating manual 9 Important information 16 Improper loading 42 Infectious substances 49 Inflammable substan...

Page 78: ...32 Quitting the modification mode 32 R Radioactive substances 19 46 Radius 14 Range of accessories 57 Reaching the maximum number of cycles 38 Relative centrifugal force RCF 14 33 Removal of the tran...

Page 79: ...corrosion 48 Structural changes 19 Supply voltage 26 Switching the centrifuge off 41 Switching the centrifuge on 27 System check 22 System error 42 44 T Table of error codes 44 Table of rotors and ac...

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