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Sigma 2-16PK, Operating Manual

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Sigma 2-16PK Operating Manual Download Page 15

 

Operating Manual SIGMA 2-16PK 

 

 

page 15 of 77 

05/2007

 

1.4 Scope 

of 

Supply 

 

The centrifuge comprises: 

 

• 

Connecting cable 

Part no. 269 010 

• 

Rotor wrench 

Part no. 930 100 

• 

20 ml slushing oil 

Part no.   70 104 

• 

1 tube of grease for load-bearing bolts 

Part no.   70 284 

 
 
 

Documentation: 

 
Operating manual incl. 

• 

EU Declaration of Conformity (page 5) 

• 

Equipment Decontamination Certificate (page 69) 

 
 
 

Accessories 
 

according to your order, our order confirmation, and our delivery note. 
 
 
 
 
 

1.5 

Standards and Regulations 

 
Please refer to the enclosed EU Declaration of Conformity (page 5). 

Summary of Contents for 2-16PK

Page 1: ...Refrigerated Centrifuge 2 16PK Operating Manual From Serial No 120951 Please retain for later use...

Page 2: ......

Page 3: ...hey also check the entire operation All of the settings are executed via the control panel with a coated surface that protects the device against moisture and dust In addition the interior of the cent...

Page 4: ......

Page 5: ...004 108 EG La machine d sign e ci dessous est produit et examin conforme aux directives 2006 42 EG 2006 95 EG und 2004 108 EG Bezeichnung der Maschine Laborzentrifuge Machine Laboratory Centrifuge D s...

Page 6: ......

Page 7: ...Safety 17 2 4 Safety Instructions for Centrifugation 18 2 4 1 Special Instructions 18 2 4 2 Resistance of Plastics 19 2 5 Prohibited Centrifuging Operations and Hazard Warnings 19 2 5 1 Special Hazar...

Page 8: ...9 5 2 1 Switching the Centrifuge ON 29 5 2 2 Opening and Closing the Lid 29 5 2 3 Installation of Rotors 30 5 2 3 1 Installation of Angle Rotors with a Hermetically Sealed Lid 31 5 2 3 2 Installation...

Page 9: ...Mode 46 6 2 Error Correction 46 6 2 1 No Indication on the Display 46 6 2 2 Centrifuge cannot be started 46 6 2 3 Centrifuge decelerates during Operation 46 6 2 4 Lid cannot be opened 47 6 2 5 Tempera...

Page 10: ...and Liability 55 10 Suitable Accessories 56 10 1 Graphical Representation of the Rotors 63 11 Appendix 64 11 1 Formulae Mathematical Relations 64 11 1 1 Relative Centrifugal Force RCF 64 11 1 2 Densi...

Page 11: ...ting the centrifuge In addition the local rules and regulations for the prevention of accidents must be complied with 1 2 Intended Use Centrifuges are power driven machines that separate liquids from...

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Page 13: ...sec 11 h 59 min short run continuous run 10 to 40 C 50 Physical data Depth mm Width mm Height mm Weight kg EMC as per EN 61326 Noise level dBA 570 550 320 60 Class B 65 Notes by the user please fill...

Page 14: ......

Page 15: ...ng oil Part no 70 104 1 tube of grease for load bearing bolts Part no 70 284 Documentation Operating manual incl EU Declaration of Conformity page 5 Equipment Decontamination Certificate page 69 Acces...

Page 16: ...ndung On Power Marche mise sous tension Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Terre Netzstecker ziehen U...

Page 17: ...rmal Notes on Safety The operating manual is an integral part of the product Please retain the operating manual throughout the service life of the centrifuge Please hand the operating manual over to a...

Page 18: ...rve the instructions on the installation of accessories see 5 2 4 page 33 2 4 1 Special Instructions If liquids with a density 1 2 g cm3 are used reduce the speed see 11 1 2 Density page 64 Protective...

Page 19: ...ngs In the case of non compliance with the instructions the manufacturer cannot be held liable or subject to any warranty claims Only persons who have read and understood the operating manual in whole...

Page 20: ...to fall contact Service if necessary Risk of crushing Attention Ensure that all repairs are performed only by authorized and specialized personnel see 6 2 8 Service Contact page 48 2 5 1 Special Haza...

Page 21: ...of Rotors an Accessories page 34 2 7 Instructions for Emergency Situations If an emergency arises actuate the emergency switch at the exit or in the room next door see 4 2 3 Fuses Emergency Circuit Br...

Page 22: ...r the centrifuge from the side Install the transport safety device foamed plastic ring into the rotor chamber Transport the centrifuge within suitable packaging and if at all possible in its original...

Page 23: ...approx 60 kg Please retain the packaging for any possible future transport of the centrifuge 4 1 1 Transport Safety Device The transport safety device of the SIGMA 2 16PK centrifuge consists of a foa...

Page 24: ...ockproof right angle plug They are equipped with temperature fuses Switch the unit off by actuating the mains power switch If they have tripped let the fuses cool down for approx 2 minutes Switch the...

Page 25: ...1 1 Operating Elements 1 2 3 4 5 6 7 8 1 Mains power switch 2 Stop key 3 Lid key 4 Lid 5 Display 6 Start key 7 Left rotary knob 8 Right rotary knob Fig 5 1 Total view of the centrifuge Fig 5 2 Rear vi...

Page 26: ...ffice 2 Type name 3 Serial number 4 Max speed 5 Kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for special disposal see chapter 8 page 51 10 CE mark in accordance with the direct...

Page 27: ...tically sealed LCD display It is operated by way of two rotary knobs and indicates the operating statuses 5 1 5 Electronic System The microprocessor controlled electronic system ensures a wide range o...

Page 28: ...the data transfer and sensor signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages with a number on the speed and rcf display see 6 2 7 Error...

Page 29: ...1 page 25 The centrifuge display then illuminates 5 2 2 Opening and Closing the Lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in or...

Page 30: ...in This must be done once a day or after 20 cycles This ensures a proper connection between the rotor and the motor shaft The rotor tie down screw should only be tightened when a rotor is installed in...

Page 31: ...r tie down screw at 5 Nm using the supplied rotor wrench so that the spring washer assembly is compressed tightly The rotor can also be used without a cover Slightly grease the rotor and lid seals aft...

Page 32: ...m at one end with putty or by fusion Place the capillary tubes into the recesses of the rotor with the sealed end against the rubber ring Ensure that the capillary tubes fit tightly against the rubber...

Page 33: ...talled symmetrically so that the buckets and their suspensions are loaded evenly Centrifugation with different tube sizes Working with different tube sizes is possible In this case however it is very...

Page 34: ...essels must be filled up to their useful volume the volume stated for the vessel If the vessels are only partially filled they will deform This may result in leaks at the seals that may become loose W...

Page 35: ...t function is used to extend the acceleration time whereas the softstop function is used to extend the deceleration time The current combination is shown on the display The stop key can be used to cyc...

Page 36: ...of the display Fig 5 10 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired duration is displayed Press the right rotary knob...

Page 37: ...e short run is displayed in the time display and the duration of the short run is also displayed Fig 5 11 When the start key is released the centrifuge decelerates at maximum power to a standstill 5 3...

Page 38: ...ight rotary knob Set will now flash Turn the right rotary knob until the desired value is displayed Press the right rotary knob to confirm the selected value If this is not done the value will be auto...

Page 39: ...nt of the parameter rcf on the display Fig 5 14 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired value is displayed Press...

Page 40: ...he value will be automatically reset to the last setting The temperature scale shows the actual temperature in the rotor chamber If the selected temperature set temperature is below the actual tempera...

Page 41: ...nd that the rotor depending on its type will reach this temperature only after a corresponding precooling time Open the lid in order to insert the samples The refrigeration unit will stop so that no w...

Page 42: ...he display Fig 5 18 Select the option by pressing or turning the right rotary knob Set will now flash Turn the right rotary knob until the desired rotor number is displayed Press the right rotary knob...

Page 43: ...ntil the desired program number is displayed Empty storage location numbers flash Press the right rotary knob to confirm the input The settings are now saved under this program number program number 8...

Page 44: ...down The parameter settings can also be locked with the help of the start key when the lid is open Press the start key three times and on the third time hold it down for approx two seconds After the a...

Page 45: ...every change the current status of the setting is displayed in the form of a message running over the display Auto Lid Open on or off 5 3 9 Activating Deactivating the Sound Signal If this function i...

Page 46: ...and line voltage present Plug in power cord correctly Fuse OK Switch unit off and let fuses cool down for approx 2 min Mains power switch on Switch on power Lid closed Close lid see 6 2 4 Lid cannot b...

Page 47: ...ach the set temperature value Check clean the condenser see 7 1 5 page 52 If the error occurs again call the service 6 2 6 Emergency Lid Release In the event of a power supply failure it is possible t...

Page 48: ...ce 50 59 Lid error press lid key close lid remove foreign matter from the opening of the lid lock device With error 50 and 51 the centrifuge will stop 60 69 Process error allow to slow down power off...

Page 49: ...intensive UV radiation or thermal stress e g by heat generators 7 1 1 Centrifuge Disconnect the power cord from the socket before cleaning Carefully remove all liquids including water and particularly...

Page 50: ...ination agent depending on the type of contamination Take suitable precautions for your own safety if there is a risk of toxic radioactive or pathogenic contamination Dry the accessories with a soft c...

Page 51: ...e signs Check the material regularly at least once a month for cracks visible damage of the surface pressure marks signs of corrosion other changes Check the bores of the rotors and multiple carriers...

Page 52: ...nce Glass particles in the centrifuge chamber will cause metal abrasion due to the strong air circulation This metal dust will not only pollute the centrifuge chamber rotor and materials to be centrif...

Page 53: ...2 Sterilization and Disinfection of the Rotor Chamber and Accessories Use commercially available disinfectants such as for example Sagrotan Buraton or Terralin available at chemist s shops or drugsto...

Page 54: ...cles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30 40 20 Polypropylene tubes 115 118 30 40 30 Teflon tubes 134 138 3 5 100 Aluminum rotors 134 138 3 5 Polycarbonate Polyallomer lids for angl...

Page 55: ...ration of decontamination Please comply with any other applicable local rules and regulations 8 2 Disposal of the Packaging Use the packaging to return the centrifuge for disposal Or Dispose of the pa...

Page 56: ...e 30 Attention Rotors from batch number 201 00 may be run with lid rotors of older batches e g 15 00 or 99 have to be run without lid 15 300 20 414 12141 Angle rotor 10 x 10 12 ml for sealed tubes e g...

Page 57: ...us 9 5 cm min radius 3 cm angle 28 9 000 8 603 13060 Adapter for 1 culture tube 15 ml Nunc Corning Falcon Greiner e g 15115 suitable for 12151 13150 13079 Bottom adapter for 1 tube 40 42 ml 15051 1505...

Page 58: ...r 17925 17950 5 000 3 773 17925 Adapter for 1 glass tube 25 ml 15025 15026 suitable for 13097 17950 Adapter for 1 tube 50 ml 15049 15050 15056 suitable for 13097 13041 Bucket aluminium incl screw cap...

Page 59: ...4 cm min radius 4 3 cm 5 000 4 025 17151 Sealing cap polysulfone clear for 13150 13060 Adapter for 1 culture tube 15 ml 15115 suitable for 12151 13150 19776 13152 Multiple carrier aluminum for 2 cult...

Page 60: ...0 Polycarbonate tube with screw cap 12 ml 16 1 x 81 1 mm suitable for 12141 13059 15000 ditto Teflon 15039 ditto polypropylene 15020 Polystyrene tube 15 ml 17 x 100 mm suitable for 11191 12072 12073 1...

Page 61: ...tubes Part no Description 15007 Centrifuge glass tube 7 ml 12 x 100 mm suitable for 13004 14029 15027 ditto graduated 0 5 5 ml in steps of 0 1 ml 15015 Centrifuge glass tube 15 ml 16 x 100 mm suitable...

Page 62: ...lass tubes microtubes culture tubes fluoropolymer tubes and especially high volume tubes can be used in our rotors buckets and adapters at higher speeds than their breaking limit Always fill the tubes...

Page 63: ...l representation of the rotors shows the maximum and minimum radii of the accessories used If necessary the values must be manually calculated see 11 1 1 RCF page 64 Fig 10 3 Microhematocrite rotor Fi...

Page 64: ...If then the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is altered the speed will be adapted accordingly under the consideration of the radius r radius in...

Page 65: ...Operating Manual SIGMA 2 16PK page 65 of 77 05 2007 Fig 11 1 Speed gravitational field diagram...

Page 66: ...7 and the Declaration of Decontamination page 69 serve for maintaining the safety and health of our employees Fill out the forms and attach them when returning centrifuges accessories and spare parts...

Page 67: ...eclaration YES NO Decontamination declaration inside Unit component contaminated Unit component unused Thank you very much for a brief problem description This may reduce the repair costs Please make...

Page 68: ......

Page 69: ...acteristics of the substances Caustic Toxic Corrosive Explosive Biologically hazardous Radioactive In Combination with which substances can hazardous mixtures develop Names of the substances Have the...

Page 70: ......

Page 71: ...90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylate C7H12O2 100 1 2 4 2 3 4 4 4 1 1 Butyl alcohol nor...

Page 72: ...roxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H2S 10 1 1 1 1 1 1 1 3 1 1 Iodine tincture of I2 1 4 3 1 1 4 4 1 1 Isopropyl alcohol C3H8O 100 1 1 1 1 1 1 1 4 1 2 Lactic acid C3H6O3 3 1 3 1 2 1 1 2...

Page 73: ...1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H...

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Page 75: ...33 Centrifuges definition 11 Chemical reactions 51 Cleaning agents 49 50 Compressor 41 Condensation 24 Condenser 53 Connected load 13 Connecting cable 15 Contamination contamination with harmful subst...

Page 76: ...l emissions 21 Microhematocrite rotor 32 Motor shaft 21 23 30 31 32 49 Multiple carrier 34 51 Noise level 13 Nominal voltage 13 Operating voltage 24 Operational safety 20 50 Overseas shipping 22 Padlo...

Page 77: ...azard warnings 30 31 Serial number 5 13 Set temperature 40 Short run 27 37 Slushing oil 15 50 51 Solvents 19 49 50 Sound signal 28 45 46 Spare parts 48 66 Storage temperature 13 22 Stress corrosion 51...

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