Shodex SUGAR KS-800 Series Operation Manual Download Page 2

Operation Manual Shodex SUGAR KS-800 series 

 

Version 2020 

 

Operation Manual 

Shodex™ SUGAR™ 

KS-800 series 

 

1. 

Introduction 

Shodex  SUGAR  KS-800  series  are  packed  with  ion  exchange  resin  gels  of  sulfonated  styrene-
divinylbenzene  copolymer.  They  are  designed  for  high-speed  and  high-performance  liquid 
chromatography.  They  are  capable  of  separating  carbohydrates  such  as  monosaccharides, 
disaccharides, oligosaccharides and sugar alcohols, as well as non-ionic water-soluble substance, with 
distilled water or 0.001 M sodium hydroxide aqueous solution used as the mobile phase. 
The columns demonstrate an excellent performance, particularly, in high-speed analysis of saccharides 
present in living bodies, food, pharmaceuticals and industrial wastewater. 
The columns fulfill USP L22 and L58 requirements. 
 

2. 

Specifications  

Product 

code 

Product name 

Counter 

ion 

Particle 

size (µm) 

Exclusion limit 

(Pullulan) 

Plate 

number 

(TP/per 

column) 

Size (mm) 

Shipping 

solvent 

F6378010  SUGAR KS-801 

Sulfo (Na

+

1,000 

 17,000 

8.0 x 300 

H

2

F6378020  SUGAR KS-802 

Sulfo (Na

+

10,000 

 17,000 

8.0 x 300 

H

2

F6378025  SUGAR KS-803 

Sulfo (Na

+

50,000 

 17,000 

8.0 x 300 

H

2

F6378035  SUGAR KS-804 

Sulfo (Na

+

400,000 

 17,000 

8.0 x 300 

H

2

F6378050  SUGAR KS-805

*

  Sulfo (Na

+

17 

5,000,000 

 9,000 

8.0 x 300 

H

2

F6378060  SUGAR KS-806

*

  Sulfo (Na

+

17 

*(50,000,000) 

 9,000 

8.0 x 300 

H

2

F6700020  SUGAR KS-G 6B  Sulfo (Na

+

10 

(guard 

column) 

6.0 x 50 

H

2

* columns marked with asterisk are available until end of 2021 

 
Packing material: 

Styrene divinylbenzene copolymer 

 

 

 

Sulfonated strong cation exchange resin gel with sodium ions 

Column material: 

SUS 316 (stainless steel) 

Screw type: 

 

Internally-threaded type No. 10-32 UNF 

*() 

 

 

Estimated value 

 

Usable conditions 

Mobile phase 

Column temperature 

Flow rate 

Column pressure 

H

2

O or 0.001 M NaOH aq. 

Max. 20% ethanol 

20 - 29°C 

0.5 mL/min 

Max. 5 MPa 

30 - 49°C 

1.0 mL/min 

50 - max. 85°C 

Max. 1.5 mL/min 

 
The columns can also be used in row. 

Summary of Contents for SUGAR KS-800 Series

Page 1: ... Shodex SUGAR KS 800 series Europe Middle East Africa Russia www shodex de Sales Office Showa Denko Europe GmbH Shodex Business Konrad Zuse Platz 3 81829 Munich Germany Technical Support info shodex de 49 0 89 93 99 62 37 Quotes and Orders order shodex de 49 0 89 93 99 62 34 ...

Page 2: ...r ion Particle size µm Exclusion limit Pullulan Plate number TP per column Size mm Shipping solvent F6378010 SUGAR KS 801 Sulfo Na 6 1 000 17 000 8 0 x 300 H2O F6378020 SUGAR KS 802 Sulfo Na 6 10 000 17 000 8 0 x 300 H2O F6378025 SUGAR KS 803 Sulfo Na 6 50 000 17 000 8 0 x 300 H2O F6378035 SUGAR KS 804 Sulfo Na 7 400 000 17 000 8 0 x 300 H2O F6378050 SUGAR KS 805 Sulfo Na 17 5 000 000 9 000 8 0 x ...

Page 3: ...n such a way that the arrow mark on the column will point to the direction of the flow Caution Do not let the air into the column during the connection 4 Heat the column to 40 50 C and start the pump Caution i Keep the column pressure below 5 MPa per column otherwise the column will be ruined ii The maximum working flow rate depends on the working temperature as follows Working temperature Maximum...

Page 4: ...3 Cap both ends of the column place it back in the same case in which it was delivered from the manufacturer and store the case in a place where the temperature does not vary more than 5 C from the ambient temperature Caution i Do not remove the end fittings of the column otherwise its performance will deteriorate or the column may be rendered unserviceable ii Do not let the in column solvent free...

Page 5: ...S 802 2 5 ethylene glycol aqueous solution 5µL KS 803 0 5 ethylene glycol aqueous solution 15 µL KS 804 0 8 glucose aqueous solution 5 µL KS 805 0 4 glucose aqueous solution 5 µL KS 806 0 8 glucose aqueous solution 5 µL 2 Mobile phase distilled water 3 Flow rate 1 0 mL min 4 Column temperature 50 C 5 Detector High sensitivity refractive index detector such as Shodex RI 6 Calculation formula NTP 5 ...

Page 6: ... following cases 3 1 If the Shodex product is not used in accordance with the operating manual 3 2 If the Shodex product is remodelled by anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not con...

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