ver.210702E
SHOWA DENKO K.K. (https://www.shodex.com/)
4
Method 1: Apply after using the column under highly aqueous condition.
Introduce water/acetonitrile = 20/80.
Method 2: Apply to samples containing proteins.
Introduce water/acetonitrile = 50/50 - 30/70 (containing 0.1 % TFA).
Attention!
· Some proteins may precipitate in high-concentration organic solvents.
· Keeping acidic solution in the column for a long time will lead to the column deterioration.
Complete the suggested column cleaning steps all the way up to the eluent replacement
at once and do not hold the procedure in middle way.
8. Column Storage
Remove the column from the system after replacing the in-column solvent with the shipping solvent. Securely
tighten the end caps and store the column at a location with stable temperature (a cool and dark space is
recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent.
Attention!
· Never allow inside of the column to dry. It can damage the column.
9. Column Inspection
Inspection method is described in the Certificate of Analysis (CoA).
Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations.
(1) Theoretical Plate Number (N)
(2) Asymmetry Factor (Fas)
10. Additional Warnings
(1) Do not remove end fittings.
(2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface.
Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications.
For additional assistance, contact the distributor from whom you purchased the column or contact your regional
Shodex
support office (https://www.shodex.com/en/support_office/list/).