Shodex Silica C18M 4D Operation Manual Download Page 1

ver.210702E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 Silica C18M 4D, 4E 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column adhesive label, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 Silica C18M series is a monomeric type ODS column 

filled with high purity silica gel modified with octadecyl group. 
 

2. Column Components   

 
 

                 

 

 

 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size (

μ

m) 

Theoretical Plate Number 

(Per Column) 

I.D. Length 

F6650040 

Silica C18M 4D 

4.6 150  5 

 10,000 

F6650041 

Silica C18M 4E 

4.6 250  5 

 16,000 

 

Base Material 

: Spherical porous particles of silica modified with octadecyl group

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Water/Methanol = 30/70 
 

4.

 

Usable Conditions 

 

 
 

Product Name 

Recommended  

Flow Rate (mL/min) 

Maximum 

Pressure (MPa) 

(Per Column) 

pH 

Range 

Temperature (°C) 

Recommended Maximum 

Silica C18M 4D 

1.0 

20 

2 - 7.5 

20 - 50 

60 

Silica C18M 4E

 

1.0 

Summary of Contents for Silica C18M 4D

Page 1: ...check if product name and serial number written on the column package column adhesive label and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex Silica C18M series is a monomeric type ODS column filled with high purity silica gel modified with octadecyl group 2 Column Components 3 Column Specifications Product Code Prod...

Page 2: ...ication system or water from a newly opened HPLC grade distilled water bottle Use HPLC grade organic solvent whenever possible Solvents left in an opened bottle for a long time should not be used The content may have been changed absorbed moisture or has been contaminated Always use freshly prepared solvents Solvents stored for a long time may have changed their compositions and may influence elut...

Page 3: ...d deteriorates the column Note It is recommended to set the pump limiter to avoid exceeding the maximum pressure 7 3 Solvent Exchange 1 When replacing the solvent start the system at less than 0 5 mL min Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume 2 Check miscibility solubility of the desired new solvent and the solvent currently filled in the column 3...

Page 4: ...ten the end caps and store the column at a location with stable temperature a cool and dark space is recommended Refer to section 7 3 Solvent Exchange for how to replace the eluent Attention Never allow inside of the column to dry It can damage the column 9 Column Inspection Inspection method is described in the Certificate of Analysis CoA Theoretical Plate Number N and Asymmetry Factor Fas were c...

Page 5: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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