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Shodex RSpak DM-614, Operation Manual, Page: 5 / 6

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Shodex RSpak DM-614 Operation Manual Download Page 5

ver.210707E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

5 

9. Column Inspection

 

Inspection method is described in the Certificate of Analysis (CoA).   
Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations.   

 

(1) Theoretical Plate Number (N) 

 

 

(2) Asymmetry Factor (Fas) 

 

 

 

10. Additional Warnings 

(1) Do not remove end fittings. 

 

(2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. 

 

Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications.   
For additional assistance, contact the distributor from whom you purchased the column or contact your regional 
Shodex

 support office (https://www.shodex.com/en/support_office/list/). 

 
 

 

Summary of Contents for RSpak DM-614

Page 1: ...1 Please visually inspect the package and outside of the column for any damage 2 Please check if product name and serial number written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex RSpak DM 614 is a polymer based reversed phase chromatography column It can be used in a wide ...

Page 2: ...ances may result in deterioration of the column and or they appear as noise on the chromatograms Filter the eluent with a 0 45 μm disposable filter to prevent the problems Attention Whenever water is required use ultra pure water freshly generated by a water purification system or water from a newly opened HPLC grade distilled water bottle Use HPLC grade organic solvent whenever possible Solvents ...

Page 3: ... the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the column side of the end fitting Presence of an extra space will let the sample to spread out and may result in wide peaks 3 Set the initial flow rate at less than 0 3 mL min and start the system If using the column at an elevated temperature keep a low flow rate until the tempera...

Page 4: ...the column If a guard column is used with the analytical column first remove the guard column and check the performance of the analytical column alone If the problem is solved most likely the cause is from the guard column In this case clean the guard column If the problem is not solved by removing the guard column clean both guard and analytical columns Make sure to clean the guard and the analyt...

Page 5: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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