ver.211201E
SHOWA DENKO K.K. (https://www.shodex.com/)
4
Method 1: Adsorption of hydrophilic compounds
Washing Solution
Cleaning Time
1 H
2
O
30
min
2
4 mM Methanesulfonic acid aq. solution/CH
3
CN = 70/30
100 min
3 H
2
O
60
min
4 Eluent
100
min
Method 2: Adsorption of hydrophobic compounds
Washing Solution
Cleaning Time
1 H
2
O
30
min
2
20 mM Methanesulfonic acid aq. solution
60 min
3 H
2
O
30
min
4 Eluent
100
min
8. Column Storage
Remove the column from the system after replacing the in-column solvent with the shipping solvent. Securely
tighten the end caps and store the column at a location with stable temperature (a cool and dark space is
recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent.
Attention!
· Never allow inside of the column to dry. It can damage the column.
9. Column Inspection
Inspection method is described in the Certificate of Analysis (CoA).
Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations.
(1) Theoretical Plate Number (N)
(2) Asymmetry Factor (Fas)