scinco Mega-900 UV-Vis Instruction Manual Download

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Mega-900

SCINCO

Introduction

Thanks for your purchase of the SCINCO CO., LTD. Mega-900 spectrophotometer.

UV-Visible Spectrophotometer is a well-accepted, documented technique with many

applications. The technique is extensively used for the analysis of agricultural

products and is widely used in public health, environmental protection, life sciences

industries and many other organic and biochemical applications.

For obtaining maximum performance and trouble-free operation, we give you the

following suggestions:

◆

Upon receiving your instrument, please check all the items listed in the

packing list are included and install the instrument according to the instruction

manual.

◆

The spectrophotometer is a precise electronic instrument. Please read the

instruction manual carefully before operation. If the equipment is used in a

manner not specified by the manufacturer, the protection provided by the

equipment may be impaired.

◆

Avoid collision and handle the instrument carefully during transportation.

◆

If the tested solution is harmful to humans, please wear gloves or use other

protection methods.

◆

Please keep the accessories in a safe place for the use in future maintenance.

For more information, please visit

www.scinco.com

Summary of Contents for Mega-900 UV-Vis

Page 1: ...0 5 Mega 900 UV Vis Spectrophotometer Instruction Manual Janurary 2016 ...

Page 2: ...ns Upon receiving your instrument please check all the items listed in the packing list are included and install the instrument according to the instruction manual The spectrophotometer is a precise electronic instrument Please read the instruction manual carefully before operation If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may b...

Page 3: ...ell holder 5 1 4 Specifications 6 Chapter 2 Installation 7 2 1 Installation location 7 2 2 Installation 7 2 3 Start 8 2 4 Performance inspection in the installation process 10 2 4 1 The accuracy and reproducibility of wavelength 10 2 4 2 Flatness of the baseline 11 Chapter 3 Instrument operation 13 3 1 About double beam measurement 13 3 1 1 What s the double beam 13 3 1 2 How to use double beam in...

Page 4: ...cell holder 28 4 7 MR19 1 Specular reflection accessory 29 4 8 MS19 1 Manual 8 cell holder 30 4 9 PS19 2 Sipper pump 31 4 10 S19 1 Solid sample holder 33 4 11 SS19 1 Short pathlength cell holder 34 4 12 TR19 1 Test tube cell holder 35 Chapter 5 Maintenance 36 5 1 Points for attention 36 5 2 Daily care 36 5 3 Trouble shooting and maintenance 36 Appendix 1 Light source replacement 39 Replace the tun...

Page 5: ...ween different electronic energy level this method can be used for qualitative and quantitative analysis of organic and inorganic compounds Lambert Beer s law is the basic law of radiation absorbance it s the foundation of spectrophotometer analysis method The absorbance A of solution is directly proportional to the path length b and the concentration c when the wavelength of the incidence light i...

Page 6: ...in the absorbance law is negative logarithm to the concentration C from the curve of negative logarithm we can see the relative error of the same transmittance in different concentration is different The relative error is very big when the concentration is too high or too low so we should choose the proper absorbance range to decrease the relative error of the result In practice we can adjust the ...

Page 7: ...t overview 1 3 1 Front view The front view is shown as Figure 1 2 the name and function of all components is described below Figure 1 2 Front view Instrument cover Front baffle of sample compartment standard front baffle for sample compartment It should be replaced when using some accessories Chassis the steel made chassis can greatly reduce vibration and ensures the credibility of the instrument ...

Page 8: ...ew is shown as Figure 1 3 the name and function of all components is described below Figure 1 3 Rear view Fan Foot packing Shockproof rubber washer is used RS 232 interface Communicate with PC Power supply Connect to power supply AC 220V 50Hz Fuse 1 Can be replaced refer to Appendix 3 fuse replacement Fuse 2 Can be replaced refer to Appendix 3 fuse replacement Instrument nameplate 1 3 3 Side view ...

Page 9: ...general configurations are fixed cell holder manual 8 cell holder and long path length cell holder We can also provide you multiple kinds of sample cell holders for your selection Please refer to chapter four Accessories for the detailed information The following figure 1 5 shows the fixed cell holder which is used as standard configuration This kind of sample cell holder is the most general struc...

Page 10: ...hin the scope of 322 392nm at 1nm interval Photometric system Double beam dynamic feedback direct ratio recording system Photometric scope 4 4 Abs Recording scope Absorbance 9 999 9 999 Abs Transmittance 999 9 999 9 T Photometric Accuracy 0 3 T 0 100 T Photometric reproducibility 0 1 T 0 100 T Stray light 0 01 T 220nm NaI 0 05 T 340nm NaNO2 Baseline flatness 0 001 Abs scan with 850 200nm Stability...

Page 11: ...from a strong electromagnetic interference source or harmful or poisonous gases Power Supply 220V 10 50Hz 1Hz A voltage regulator 500VA Ensure satisfactory earth connection for the instrument 2 2 Installation MEGA 900 is composed of the three major components A UV Vis spectrophotometer main unit An IBM compatible Personal Computer completed with a monitor and a keyboard For the computer outfitted ...

Page 12: ...trument PC and printer to the power supply connector Note make sure that the rating of the power source be an AC of 220 V with 50 HZ Note The spectrophotometer is a precision electronic instrument Don t pull out or insert the power cable or tie cable when the instrument is on otherwise it will destroy the instrument and the computer 2 3 Start Be sure to there is nothing to block in the sample cell...

Page 13: ...ting lasting 60 minutes after switching on the instrument in order to insure the precision of result During this time of initialization we advise you being familiar with the operation of the instrument and software by instruction manual on the back If you need the instruction manual of the LabPro Scan software please refer to the software manual and help document of the software menu After the mea...

Page 14: ...er 1 4 Specification The checkup should be made in the following manner by using the two characteristic spectrum lines of the deuterium lamp Setting the parameters by using the spectrum scan function refer to LabPro Scan software manual as shown in table 2 1 and table 2 2 Table 2 1 656 1nm characteristic spectrum Scan parameter Instruction Photometric Es Energy for sample light Scan speed Medium S...

Page 15: ... 1 Formula n i i n 1 1 2 1 i measured value of wavelength n measured times here n 3 The difference value between the mean value and standard value is the wavelength accuracy The difference value between the maximum and the minimum is the wavelength reproducibility 2 4 2 Flatness of the baseline Testing method Check that the sample beam is blank empty with no solution Then set the measuring paramet...

Page 16: ...velength as 850 200nm and start for spectrum scanning Read the absorbance of the spectrogram by the graph reading function The largest absorbance should conform to the requirement of the flatness of the baseline A sudden change at the wavelength of lamp changeover is permitted ...

Page 17: ... beam UV Vis spectrophotometer takes two light beams who s energy are the same to measure sample and reference respectively at the same time as it s optical principle as figure 3 1 shown R means reference light beam and S means sample light beam increase the stability and precision of the instrument by the stability of real time feedback compensation measure system of the reference beam The most d...

Page 18: ...ngle beam you should put the blank and sample into the sample cell holder to measure at twice when zero and measurement while to double beam just once To double beam instrument you just have to put the black block into sample light beam cell holder to do the dark current correction You can familiar with the operation of Mega 900 by the following several application examples Reference cell holder S...

Page 19: ...nstrument Power on the PC which connect with the Mega 900 enter into the Windows operate system Be sure to there is nothing to block in the sample cell holder and power on the instrument start LabPro Scan software carry through self checking After self checking enter main working program The measurement can only be done for an interval of preheating lasting 60 minutes after switching on the instru...

Page 20: ...sample light beam cell holder cover the sample compartment cover press Start button of the LabPro Scan software to finish the measurement After finishing measurement one time press Start again to measure the next data We can get the result by the averaging of several measurements Analyze the result You can save the result as special file format of LabPro Scan software for checking and printing lat...

Page 21: ...Pro Scan software Take out the black block after correction Notice Dark current correction can eliminate some noise of the instrument and insure the precision of result You don t have to do it when at every measurement just when the environment has been changed such as the temperature changed to much the location has been changed measuring high absorbance sample and so on Advise you doing the dark...

Page 22: ...esult You can use the Peak picking function of software to analyze the spectrum after finishing the measurement By shortcut of Peak picking or under the Graph menu You can search the peak by peak picking function if not you can set the threshold The software will list the result of measurement correspond to the peaks after peak picking Result output You can save the result of spectrum and peak pic...

Page 23: ...s re prepare it Dispose the sample Dispose the electronic and electrical products have been split into strip or powder sample which the diameter no more than 1 mm and length no more than 5 mm by crusher Weight 0 5 2 5 g precision to 1mg sample exactly into conical flask add into 25 0 mL leaching solution and 0 5 mL buffer solution and the sample should be submerged in the leaching solution and mix...

Page 24: ...riol solution 1 9 make the pH value of the filtrate in the range of 1 5 to 2 5 constant volume with water The concentrations of Cr Ⅵ in these calibration solution are 0 0 0 1 0 2 0 3 0 4 0 5 0 6mg L Mixed and shake and placed for half an hour Sample solution Add 2 0mL Diphenylcarbazide color producing reagent into sample filtrate mixed and drop vitriol solution 1 9 make the pH value of filtrate in...

Page 25: ... Abs in the real time At last the calibration curve will be finished as below shown Table 3 3 Calibration Curve The parameters of calibration curve is Abs K1 C K0 K0 0 0014 K1 0 68847 R2 0 9999 Measure the sample Input the ID of sample in the Sample interface under the same condition and measure the Abs of sample solution and blank solution then the software will calculate the concentration of Cr ...

Page 26: ...ncentration of leaching solution by calibration curve the unit is mg L B the concentration of blank solution by calibration curve the unit is mg L V the constant volume of leaching solution the unit is mL N the dilution multiple of leaching solution M the mass of sample the unit is gram g ...

Page 27: ... can measure samples at a specific temperature The picture is as figure 4 1 shown the specification is as Table 4 1 shown Figure 4 1 Thermostatic cell holder Table 4 1 specifications NO Item Specifications Remark 1 Product model CH19 1 2 Connection tube Inner diameter Φ4mm 3 Wavelength range 190 900nm 4 Path length 10mm 5 Cell holder NO 2 Sample beam and reference beam sample holder ...

Page 28: ...sted within 45 It is easy to measure the absorbency for solid samples from multi angles The picture is as figure 4 2 shown the specification is as Table 4 2 shown Figure 4 2 Angle changeable solid sample holder Table 4 2 Specifications NO Item Specifications Remark 1 Product model DS19 1 2 Wavelength range 190 900nm 3 Changeable angle range 45o 4 Min sample size φ4mm 5 Max sample size 80x55x5mm ...

Page 29: ...lor difference and whiteness The picture is as figure 4 3 shown the specification is as Table 4 3 shown Figure 4 3 Integrating sphere Table 4 3 Specifications NO Item Specifications Remark 1 Product model IS19 1 2 Incident angle Sample light beam 0 Reference light beam8 3 Min sample dimensions Width 15mm Height25mm Diffuse reflectance 4 Min sample dimensions Diameter 20mm Transmittance 5 Wavelengt...

Page 30: ...es with long pathlength The picture is as figure 4 4 shown the specification is as Table 4 4 shown Figure 4 4 Long pathlength cell holder Table 4 4 Specifications NO Item Specifications Remark 1 Product model LS19 1 2 Wavelength range 190 900nm 3 Pathlength 5mm 10 mm 20 mm 30 mm 40 mm 50 mm 100 mm Adjust according to needs 4 Cell NO 2 Sample light beam and reference light beam ...

Page 31: ... cell holder With micro cuvettes it can improve measurement precision for micro sample The picture is as figure 4 5 shown the specification is as Table 4 5 shown Figure 4 5 Micro cell holder Table 4 5 Specifications NO Item Specifications Remark 1 Product model MH19 1 2 Wavelength range 190 900nm 3 Cell NO 2 ...

Page 32: ...used to shrink imaging of beam in the sample and reference optical circuits of the ultra micro cell holder After the beam passes through the ultra micro cell holder it will be magnified by quartz lens to collect energy and shorten the facula The signal noise ratio and measurement precision of the instrument will be improved greatly The picture is as figure 4 6 shown the specification is as Table 4...

Page 33: ...e reference beam is the standard reflecting mirror the user can calculate the absolute reflectivity of the measured surface The picture is as figure 4 7 shown the specification is as Table 4 7 shown Figure 4 7 Specular reflection accessory Table 4 7 Specifications NO Item Specifications Remark 1 Product model MR19 1 2 Incidence angle 5o 3 Measured sample area 11 9mm to 60 40mm 4 Wavelength range 1...

Page 34: ...mples can be put in the cell holder for separate measurement The picture is as figure 4 8 shown the specification is as Table 4 8 shown Figure 4 8 Manual 8 cell holder Table 4 8 Specifications NO Item Specifications Remark 1 Product model MS19 1 2 Wavelength range 190 900nm 3 Cell NO 9 Reference cell holder 1 Sample cell holder 8 ...

Page 35: ...the last operation when it is turned on e 3 It has new control system with jiggle switch and quantitative sampling system There are two working modes continual and quantitative It can be used for continuous sampling and discontinuous quantitative sampling 4 It has an auto calibration function Users can calibrate the sampling volume through the calibration function in order to avoid the error after...

Page 36: ... range 0 1 250rpm Clockwise counterclockwise 3 Speed resolution 0 1rpm lower than 30rpm 1 rpm higher than30rpm 4 Display mode 3 1 2 LCD display 5 Sampling accuracy 1 mL 6 Power supply AC85v 264v 50 60Hz 7 Working environment Temp 0 400C Relativity humidity 80 8 Driver size L W H 370 210 500 mm 9 Driver weight 10kg ...

Page 37: ...optical circuit It is easy to measure the absorbency for solid samples The picture is as figure 4 10 shown the specification is as Table 4 10 shown Figure 4 10 Solid sample holder Table 4 10 Specifications NO Item Specifications Remark 1 Product model S19 1 2 Wavelength range 190 900nm 3 Cell NO 2 4 Max sample size 80x55x5mm ...

Page 38: ...pathlength cell holder can be used in the following holders standard fixed cell holder 8 cell holder micro cell holder ultra micro cell holder and etc The picture is as figure 4 11 shown the specification is as Table 4 11shown Figure 4 11 Short pathlength cell holder Table 4 11 Specifications NO Item Specifications Remark 1 Product model SS19 1 2 Wavelength range 190 1100nm 3 Path length 1mm 2mm 5...

Page 39: ...al and precision for test tubes If precise measurement is needed it is recommended that the related accessory for cuvettes be used The picture is as figure 4 12 shown the specification is as Table 4 12shown Figure 4 12 Test tube cell holder Table 4 12 Specifications NO Item Specifications Remark 1 Product model TR19 1 2 Wavelength range 190 900nm 3 Test tube range Diameter 15 25mm Height 90 120mm ...

Page 40: ...ovided with the instrument Silica gel bags can be put in the sample compartment and the light source housing to keep dry But be sure to take the bags out before switching on the instrument Periodically check the performance of the instrument If anything abnormal occurs contact the seller or the manufacturer immediately If the instrument is not to be used for a long time pay great attention to envi...

Page 41: ... Wavelength check A Opaque object in the sample compartment B Wrong positioning of D2 lamp C WL mechanism failure there is noise A Open the sample compartment B Open the light source housing C Open the base A Remove the opaque object B Check the facula of D2 lamp C According to the following document Noise abnormality A Light source ageing A Measure in Energy mode A Replace the light source B Wron...

Page 42: ...oss stand as figure 5 1 upper one shown rotate the screws on the base disassemble the base 2 Find the scanning mechanism as the figure 5 1 shown familiar the mechanism of the instrument according to the picture Check the nut which end it stops 3 If the nut stops as the picture shown rotate the connecter counterclockwise make the nut can move freely on the guide screw If the nut stops at the other ...

Page 43: ...replace the tungsten lamp please observe the following procedures to replace it Figure 6 1 Tungsten lamp set Open the light source house cover Disassemble the two fixed screws of the light source house cover and take it off as the figure 6 2 shown Figure 6 2 Open the light source house cover Loosen the two holding screws on the tungsten lamp socket and remove the tungsten lamp set as shown in figu...

Page 44: ...one upon switching on the power First remove the tungsten lamp holder loosen the adjust screw on the tungsten lamp holder with a watch screwdriver and adjust the height of tungsten lamp As figure 6 1 shown The distance between the center of the filament and the bottom should be 40mm according to the design requirements But it can be adjusted slightly And then tighten the setscrews and insert the W...

Page 45: ...ped the light beam window should face to the convergent lens as shown in figure 6 6 Figure 6 6 Insert the D2 lamp Slightly sway the deuterium lamp and install it securely Note 1 The light source and light source housing are considerably heated during operation Therefore be sure to turn off the power switch so as to cool down the light source sufficiently before replacing it 2 The convergent lens s...

Page 46: ... instrument Remove the fuse holder by a big plain screwdriver as shown in figure 6 1 Figure 6 1 Remove the fuse Remove the old fuse and replace it with a new one And then install the fuse holder The fuse replacement has finished Figure 6 2 Replace the fuse Note The instrument takes two kinds of fuse 2A and 0 5A in case of the damage to instrument please select the same fuse as the old one when rep...

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