Submit a Sample or Set of Samples
Note:
Run the sample again in the event of an abnormal termination during sample acquisition. If the abnormal
termination is caused by a power failure, then the temperature of the autosampler tray is not maintained and
sample integrity might be compromised.
1. Select one sample or a set of samples.
2. Click the
Submit
tab in the
Batch Editor
.
3. If the
Submit Status
section contains a message about the status of the batch, then do one of the following:
• If the message indicates that the batch is ready for submission, then proceed to the next step.
• If the message indicates that the batch is not ready for submission, then make the changes as indicated by
the message.
4. Click
Submit
.
Set Up Sample Calibration
The software can automatically schedule and execute the external auto calibration while samples are being acquired
in batch mode. This ensures good mass accuracy is maintained throughout the acquisition.
If the CDS is not configured, calibration is done using an autosampler and users must supply the calibration method
(*.dam) and the vial position of the calibrant sample.
1. In the
Batch Editor
, click the
Calibrate
tab.
2. In the
Calibrate Every _ Samples
field, type the number of samples to be acquired between calibration
samples.
3. From the
Calibrant Reference Table
, select a table from the list of all calibrant reference tables available
for the current polarity. Make sure that the selected reference table has the correct
Calibrant Valve Position
.
4. Set the
CDS Inject Flow Rate
.
When the batch is submitted, the calibration samples are inserted into the queue. Each set starts with a
calibration sample. The calibration method is named AnalystCal_ plus the acquisition method name (for
example, AnalystCal_TOF.dam). If the CDS is configured, the software automatically creates a calibration
method that matches the acquisition method that is used for the next sample in the queue. Calibration data
is saved to a separate data file for each calibration sample. The calibration data file along with the calibration
report is saved in the subfolder Cal Data and named with Cal plus the time stamp and calibration sample index
(for example, Cal200906261038341.wiff) if the Keep Calibration Data File was selected in the Queue Options
dialog. The calibration report is named with Cal plus the time stamp, calibration sample index, and the word
report (for example, Cal20130822154447030_report.txt). The report displays the peak finding criteria, the
TripleTOF
®
5600/5600+ System
System User Guide
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RUO-IDV-05-7040-B
Operating Instructions — Batches
