Scientifica SliceMate, Manual, Page: 5 / 18

5
The SliceMate should ideally be sited on a stable vibration isolated table. The base
plate accommodates mounting on either 25mm or inch hole spaced table
surfaces.
For safety during transit, the SliceMate is shipped as one assembly. In order to
mount the SliceMate first unscrew the two black Acetyl knobs and lift the
SliceMate from its base plate.
The base plate can now be sited as required and screwed down using the 4
button headed screws which can be found in the accessory kit. These are either ¼
x 20 x ¾” screws if using an inch table or, M6 x 20mm if metric.
Now locate the SliceMate onto the base plate and screw down the Acetyl knobs
to secure the assembly.
Setting up the fluid delivery.
The system can be used with either a gravity fed or pumped system. The system is
designed to operate over a flow rate range of 1 to 10 ml/ minute. The fluid should
be delivered to the SliceMate using tygon (or similar) tubing with an internal
diameter of 1.6mm (1/16”). If the fluid is to be pumped Scientifica recommend the
use of a fluid break in order to minimize pulsation effects.
Minimizing bubbles
It is suggested that the solutions to be used should be pre-heated. This is generally
done by placing gassing flasks of the solution in a water bath set at approximately
5ºC higher than the desired SliceMate bath temperature. The solution is then
transferred to the perfusion vessels prior to starting the experiment.
The theory is that although the solution is being gassed it should outgas at the pre-
heated temperature and as it never gets hotter than the pre-heated temperature