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SliceMate Manual
Page 1: ...1 SliceMate Manual...
Page 2: ...e housing integrates the tissue bath heat exchanger Peltier device and fluidic control valve within a dedicated Acetyl enclosure The compact and precision design ensures system variables are minimised...
Page 3: ...he Stainless Steel ground plane of the SliceMate The heat exchanger spiral is held in position by a stainless steel spring clip this ensures the heat exchanger has ideal contact with the Peltier devic...
Page 4: ...a low resistance earth bond via the connector shells Reference Electrode Ground The reference electrode ground is located in between the primary well and the suction port in the brain bath The head st...
Page 5: ...livery The system can be used with either a gravity fed or pumped system The system is designed to operate over a flow rate range of 1 to 10 ml minute The fluid should be delivered to the SliceMate us...
Page 6: ...the vacuum The bath solution height is maintained and adjusted using the vacuum wicking method The SliceMate bath has a pair of oval suction vents which are connected to the outlet port at the rear of...
Page 7: ...done so that when the flow is turned back on there will be a minimal risk of a hot surge of fluid entering the bath and the desired temperature is quickly regained The system initialises on power up...
Page 8: ...low and allow the target temperature to stabilise Continue with the experiment End Experiment Turn the thermal control off by selecting the station number on the console handset and pressing stop Note...
Page 9: ...ith exception of the brain bath module AgCI Electrode Assembly Heat Exchanger Tube Pinch Valve Tube Feed through barbed connectors Brain bath mesh Replacing the Brain Bath Mesh Firstly carefully ease...
Page 10: ...oved from the SliceMate base Unscrew the two black Acetyl knobs in an anticlockwise direction and lift the SliceMate clear from the table Replacing the heat exchanger tubing 1 Firstly obtain a piece o...
Page 11: ...led free 6 Now install the new tubing take the angled cut end have this extending from the edge of the spiral by 6 5 to 7 5mm to allow attaching the silicon valve tubing later on Being careful not to...
Page 12: ...es lifting the brain slice during operation Remove the tubing from the inlet port to the heat exchanger by first pulling it out of the pinch valve and then pulling away from the heat exchanger and fin...
Page 13: ...ng 5 Now avoiding kinking the heat exchanger tubing form a comfortable transition from the heat exchanger into the valve jaw 6 Using a spatula or similar tool press the silicon tubing into the valve j...
Page 14: ...venient replacement with minimal tooling The silver chloride pellet is sleeved within silicon tubing this acts as both a seal between the pellet and brain bath and as a pull sleeve enabling easy insta...
Page 15: ...from the top side of the bath and then gently pulled from the underside until the surface of the pellet is level with the surface of the bath If the user prefers to make the assembly themselves the fo...
Page 16: ...or wire polythene tube assembly through a new piece of silicon tube of 35mm length 6 Solder the connector wire to AgCI Electrode ensure smooth streamline joint 7 Ensure polythene sleeve slides freely...
Page 17: ...Heat Exchanger Lid Retainer Screws Tube Cutter Silicon Tubes Steel Rule Measuring Tubing Length s Soldering Station Ref Electrode Off line Assembly Solder 5 core Ref Electrode Off line Assembly Plast...
Page 18: ...um cured silicone return heat exchanger to Outlet Port 1 8 ID platinum cured silicone Bath Earth Ag Cl pellet E201 33 0003 Harvard Bath Earth tube seal Silicone tubing 125 OD 125 ID Thermistor ALA Bea...
