•
Capacity of culture mediums and containers
Pour less liquid in the container than the specified volume.
Excessive liquid will spill when heated.
•
Sterilization of large volume culture mediums
There is a time lag between when the temperature in the chamber
reaches the sterilization temperature and when the temperature of
the whole culture medium reaches the sterilization temperature.
For this reason, this time lag has been included in the sterilization
time in advance. Therefore, take the time lag into account when
setting the sterilization time.
•
Sterilization with the use of plastic containers
Prolong the sterilization setting time even more. Otherwise, poor
heat conduction of the container may cause incomplete
sterilization.
•
Melting of solidified general agar mediums
Refer to the below table to set the melting time (per flask).
•
Test of Durham tube
Air remaining in the Durham tube can be purged by setting the
exhaust temperature and exhaust rate to appropriate levels.
Agar media in 500ml flask x 1~21, or
Agar media in 1000ml flask x 1~12
11min.
8min.
Liquid Volume
Time Lag
Melting Temperature
Capacity
2 L
100
°
C
1 L
60min.
45min.
Melting Time
An
Object temperature sensor
that detects the temperature
of objects to be sterilized and controls sterilization time is available
as an option. Even after this sensor has been installed, control can
be switched over to the product’s own sensor by pressing the
object temperature ON/OFF button. Use this optional sensor to
ensure sterilization is more reliable. For details, contact your
dealer.
Recommendation
Sterilization and melting of liquids such
as culture mediums, etc.
•
Do not sterilize objects in a steam-impermeable container or bag.
•
Do not seal the object container tightly. Use a porous cap or
loosen the cover sufficiently.
•
Do not allow the sterilization objects to block the holes or
temperature sensor in the chamber.
WARNING
MLS-3751
MLS-3781
Sterilization Setting Time = Sterilization Time + Time Lag
Porous caps
Culture medium
temperature
Time
121
°
C
100
°
C
Temperature
Sterilization
setting time
Time lag
Sterilization
time
Plastic
container
Durham tube
Temperature inside
the chamber
12
Sterilization Objects
