Qsonica SONICATOR S-4000 Operation Manual Download Page 48

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Microplate Horn Diagram (#431MPX)

Clear Acrylic
Deep Well Collar

O-ring

Microplate Horn Adapter
(Grey)

Hose Barb Connector

Microplate Horn

Tygon Tubing

Stud

Pinch Clamps

•

The tall, clear collar is removable. If it is not required, simply slide it upwards and it will be
released.

•

The Sound Enclosure is included with the purchase of the # S-4000MPX or # 431MPX.

•

If you only purchase # 431MPX, the Generator and Converter are not included.

Summary of Contents for SONICATOR S-4000

Page 1: ...SONICATOR ULTRASONIC PROCESSOR Part No S 4000 OPERATION MANUAL 53 Church Hill Road Newtown CT 06470 203 426 0101 tel 203 426 7026 fax info sonicator com www sonicator com...

Page 2: ...e Getting Started 11 14 6 Operating Instructions 15 24 7 Accessories 25 26 8 Sound Reducing Enclosure 27 29 9 Techniques for Optimizing Results 30 10 Maintenance 31 36 11 Troubleshooting 37 38 12 Warr...

Page 3: ...d the manual in its entirety The new digital Sonicator offers several new and innovative features This manual provides the necessary instruction and guidance to help ensure the successful operation of...

Page 4: ...es in temperature and humidity Do not place the Generator within a Fume Hood NEVER immerse the converter in liquids of any kind or let condensed moisture or liquid drip into the converter NEVER grasp...

Page 5: ...C 120 VAC 8 Amps Fast Acting For 220 VAC 240 VAC 4 Amps Fast Acting Weight 13 5 lbs 6 1Kg Dimensions 11 W x19 5 L x 5 25 H 27 9cm x 49 5cm x 13 3cm Output Voltage 1000 V rms max Output Frequency 20 KH...

Page 6: ...djustment See Option menu The Power Cord supplied with the Sonicator must be used If the 220V plug is not configured to match the wall receptacle a properly grounded universal AC socket adapter must b...

Page 7: ...the cavitation field The erosion and shock effect of the collapse of the cavitation bubble is the primary mechanism of fluid processing The probe tip diameter dictates the amount of sample that can be...

Page 8: ...1 100 giving a greater degree of resolution for setting the amplitude needed to effectively process samples Reference information such as converter power output joules and process time is also displa...

Page 9: ...9 The S 4000 includes a standard diameter tapped horn 200 Generator Converter Horn with replaceable tip Converter Cable...

Page 10: ...10 S 4000 Rear Panel On Off Switch Power Cord Connector Converter Cable Connector USB Connector Temperature Probe Connector Footswitch Jack Cooling Fan...

Page 11: ...at the factory Check the tightness of the horn and flat tip by using the wrench set Please refer to images on page 12 of this manual A loose horn or tip may cause damage to the generator circuitry or...

Page 12: ...Horn Tightening Tip Removal Tip Tightening Microtip Removal Microtip Tightening Wrench Set Note When tightening a Microtip the tip must not be in contact with the worksurface Always have the tip exte...

Page 13: ...secure the connectors If a foot switch or timer switch is used plug it into the foot switch connector Make sure that the plug is inserted properly Converter and horn mounted in Sound Enclosure 432B P...

Page 14: ...iquids solvents require a solid tip horn Set up regular preventive maintenance schedule See the Maintenance section for details Hearing protection is highly recommended The Sound Enclosure 432B reduce...

Page 15: ...anually performed b Program Mode Allows the user to program specific On Off times at any programmed output setting The ability to save up to 10 programs and sequence several individual programs togeth...

Page 16: ...then appears When using a Microtip the YES button must be selected Warning Improper selection will result in tip damage or poor sonication Damage caused by not following this step is not covered under...

Page 17: ...s the user to create a program select or run a program and select or run a sequence of programs Option Screen The Option menu allows for the selection of Temperature units Footswitch operational modes...

Page 18: ...intensity setting for your sample by touching the Up or Down arrow Touch Start to begin sonication Touch Pause to pause sonication Manual and Program modes both show the Start Pause and Exit buttons...

Page 19: ...erent programs Select Modify Sequence 1 2 Select a sequence of programs for the unit to run in succession A maximum of 6 programs can be sequenced at one time Option Screen The Option menu enables the...

Page 20: ...red the unit will run continuously without pulsing Process Time Pulse On and Pulse Off times are measured in Hours Minutes Seconds format 6 Activate Temperature Monitoring if necessary 7 Program a Shu...

Page 21: ...n progress The Temperature Monitoring and Shutdown Temperature option have been selected during this example program Program Progress is displayed by a bar graph This displays the progress of the curr...

Page 22: ...in the PGM fields 3 If a delay or rest time between programs is desired touch the Delay After field Enter the appropriate time frame 4 Unused PGM fields should have a dash Select unsused programs tou...

Page 23: ...must be in the liquid sample If the initial sample temperature is above the set point sonication will not turn on During sonication if the temperature reaches the Shutdown value the unit will pause so...

Page 24: ...g on the type of fault detected Below are two examples Further details are included in the Troubleshooting section The self diagnostic features of this unit apply to all standard horns and Microtips D...

Page 25: ...ates the amount of sample that can be effectively processed Smaller tip diameters Microtip probes deliver high intensity sonication but the energy is focused within a small concentrated area Larger ti...

Page 26: ...sels at one time Indirect sonication is most effective for very small samples because foaming and sample loss are eliminated Pathogenic or sterile samples are ideal for this method because aerosols an...

Page 27: ...installation of horn Note A diameter rod is mounted inside the enclosure A standard laboratory clamp can be used to hold samples if necessary A clamp is not included with the enclosure For shipping p...

Page 28: ...e assembly into the holder making sure the cable is not crimped There is a capped port on either side of the enclosure for cold water circulation during sonication See Section 14 for Cup Horn instruct...

Page 29: ...d converter must be properly tightened using the appropriate wrench set before being inserted into the sound enclosure Remove collar and insert covertor as shown Attach collar around Converter and sec...

Page 30: ...as a Cup Horn or Microplate Horn may be a better option Viscosity Limitations Viscous solutions and highly concentrated liquids can be difficult to sonicate If the liquid is so thick that it will not...

Page 31: ...dure done with the Sonicator power switch in the OFF position and with the unit unplugged from the AC power source Long exposure to acids or caustics results in corrosion of metal parts or components...

Page 32: ...eneral use the cable assembly should not be used to carry the converter or pull it toward the user Make certain the cable always has slack and is never tensioned If necessary move the generator or con...

Page 33: ...33 Clean threaded Stud Clean threading in converter Do not allow liquid to drip into Convertor Allow parts to dry Tighten Stud to horn...

Page 34: ...34 Cleaning horn stud Cleaning horn threading...

Page 35: ...d turn the power on for a few seconds to remove residue The tip also can be sterilized using alcohol with the power on Alcohol is a volatile compound and proper handling precautions must be taken whil...

Page 36: ...probe tip will increase the rate of future erosion For that reason it is recommended that after every 5 or 6 hours of use the tip be examined and if necessary polished with a fine emery cloth Since t...

Page 37: ...ve step by step instructions to help remedy the problem If the Start button does not initiate sonication please go to the Option Screen Check the Footswitch option and even if you are not using a Foot...

Page 38: ...visit our website at www sonicator com and fill out our brief Repair Form This form provides us with the necessary information to quickly diagnose your problem and will speed up the repair of your equ...

Page 39: ...plant in Farmingdale New York or at any of its designated service centers without charge including parts and labor but not including shipping charges necessitated by any defect in workmanship or mater...

Page 40: ...U2000A0CL4 both are needed The total cost for the boxes and foam is 50 00 including shipping to any US location QSonica is not responsible for shipping damage on equipment returned to our facility for...

Page 41: ...sample If the water temperature rises pour it out and refill the cup with cold water Another method of operation is to fit the outlet port with the hose adapter soft tubing and a pinch clamp Run the...

Page 42: ...rtical diaphanous streams in the center of the cup sometimes forming a spider like pattern Output Amplitude Settings When working with small samples there is a tendency to turn the Amplitude setting t...

Page 43: ...43...

Page 44: ...a clear acrylic collar to contain the liquid media within the reservoir This format allows the user to process deep well microplates or other large vessels Standard microtiter plates or pcr tubes requ...

Page 45: ...at the bottom of the Acoustic Enclosure Connect the cable to the converter and slowly feed the cord down through the bottom of the enclosure until the converter sits properly in the holder be sure tha...

Page 46: ...rough air it requires a liquid medium to be transmitted effectively If air pockets are present and water is not in contact with the bottom and side of each well sonication will be inefficient and your...

Page 47: ...ts The optimum scenario is to re circulate ice cold water with pulsed sonication Maintenance To ensure optimum performance it is highly recommended to remove the horn from the converter and clean the...

Page 48: ...Connector Microplate Horn Tygon Tubing Stud Pinch Clamps The tall clear collar is removable If it is not required simply slide it upwards and it will be released The Sound Enclosure is included with t...

Page 49: ...Corning Costar 1 800 492 1110 Chillers Peristaltic Pumps Thermo Fisher Catalog Chillers Catalog 13 874 7 Model 3006 capacity 6L Catalog 22 037 157 Model WK 230 Benchtop chiller capacity 6L Thermo Fish...

Page 50: ...her end of the body and the mating surface of the Horn and fit the body over the Horn 8 Screw the Flocell body around the Horn until the end of the horn thread contacts the orifice plate and then back...

Page 51: ...body over the Horn 8 Screw the Flocell body around the Horn until the end of the horn thread contacts the orifice plate and then back it off 1 full turn 9 The Flocell should not be tightened on the h...

Page 52: ...ure proper installation of the horn into the Focell body 7 Push horn into upper section of Flocell until fully seated 8 Place 3 sanitary gasket on upper section flange of Flocell body 9 Place each hal...

Page 53: ...53...

Page 54: ...be clean tight mating surfaces Please refer to Maintenance section of this manual for direction on how to clean the threaded mating surfaces and ensure that the threaded studs are attached tightly to...

Page 55: ...extender horns are tested in pairs at the factory to ensure balanced operation and consistent output when used together It is highly recommended to replace both horns at the same time Mixing extender...

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