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Kryo550-16

Operator’s Manual

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Summary of Contents for Kryo550-16

Page 1: ...Kryo550 16 Operator s Manual...

Page 2: ...and other components which have a limited life To ensure the long life of the equipment and help ensure trouble free operation we recommend that you join one of our Maintenance and Support Schemes Ple...

Page 3: ...s Manual Failure to comply with these instructions could result in personal injury Failure to follow these instructions could result in damage to your equipment system or samples This indicates first...

Page 4: ...erride Mains power off Mains power on Sample display key Caution Read this manual Failure to observe the instructions within this manual could result in personal injury Alarm output Reset Sample PRT C...

Page 5: ...ere is a risk of reduced or insufficient ventilation when a room is unoccupied then alarms to indicate oxygen deficiency must be installed and positioned outside of the room so that operators are awar...

Page 6: ...nuisance trips The 9 way D Type plug on the Kryo550 16 interface panel is only for connecting to the RS232 serial port of a computer which complies with IEC950 The RJ45 ethernet connector provided wi...

Page 7: ...t the plastic covers and plugs provided to all unused connectors Immediately before touching the sample PRT touch an earthed part such as the metal surface at the rear of the Kryo550 16 Do not touch a...

Page 8: ...was started from DeltaT 3 2 If the run was started from the control panel 3 2 Warming the Chamber 3 2 Removing the Samples 3 1 Loading the Samples 3 1 Starting the Run 3 1 Chapter 3 Freezing Samples 2...

Page 9: ...C 1 Safety Declaration Contents vii...

Page 10: ...viii Contents...

Page 11: ...m cells skin cord blood and other critical high volume samples This manual is intended for both operators of the freezer and those persons responsible for its installation System Components The major...

Page 12: ...pical Installation 1 2 Introduction to the Kryo550 16 Mains power Personal computer Printer Ethernet hub Alternative network connection if kryo connect card fitted Pump power supply LNP4 pump and dewa...

Page 13: ...ile Stop button Press to stop a profile Cool indicator light Illuminated when cooling Heat indicator light Illuminated when heating Power indicator Illuminated when mains power is switched on Sample k...

Page 14: ...Interface Panel 1 4 Introduction to the Kryo550 16 Reset button Manual override heat Manual override cool Sample PRT Alarm output RS232 Serial port kryo connect ethernet connector if fitted...

Page 15: ...Rear Panel Introduction to the Kryo550 16 1 5 Mains on off switch Pressure relief valve Mains power inlet Liquid nitrogen inlet...

Page 16: ...alone operation The Kryo550 16 can store a profile that can run independently of the personal computer PC See Kryo550 16 Profile Memory Heat Cool switches Manual override push button switches on the i...

Page 17: ...act your service engineer if you want the hose configuration changed Kryo550 16 Profile Memory The Kryo550 16 has two memory locations that are used for storing profiles These are the Permanent Profil...

Page 18: ...1 8 Introduction to the Kryo550 16...

Page 19: ...nternal to the building If you have the kryo connect option installed connection to a PC must be made through the Ethernet connection In this case follow the instructions in the kryo connect guide Ens...

Page 20: ...e thread with PTFE tap and fix to the LN2 input on the back of the chamber Tighten securely with a spanner Connect an appropriate adapter to the cylinder observing the manufacturer s instructions then...

Page 21: ...re that the fibre washer is fitted to this joint Tighten securely with a spanner Filling the Dewar When refilling a dewar that already contains liquid nitrogen you must first depressurise it and remov...

Page 22: ...pressure release valve Ensure that the mains power outlet to which the pump power supply is to be connected is readily accessible Disconnection of the pump power supply from the mains supply is by dis...

Page 23: ...6 Disconnect the mains supply at the wall socket Open the red pressure release valve Cold gas will issue from the valve Wait until the pressure on the gauge has fallen to zero and the flow of gas has...

Page 24: ...2 6 Installing the Kryo550 16...

Page 25: ...start to be heated or cooled until the start temperature is reached When the start temperature has been reached the Run Indicator on the control panel will start to flash and the Kryo550 16 will begin...

Page 26: ...nd not stored in the Permanent Profile memory or the program in the freezer s memory does not have a start temperature above 0 o C then a profile with a start temperature of 0 o C and a single step of...

Page 27: ...start temperature and soak time are defined in the profile When the seeding start temperature is reached the chamber temperature is maintained at that temperature for the specified soak time After thi...

Page 28: ...re the liquid nitrogen valve is opened fully for the time defined by the pulse duration This causes the temperature in the chamber to fall very quickly The sudden drop in temperature can be used to in...

Page 29: ...If the solenoid valve has stopped for more than two minutes and the profile should be cooling estimate the temperature of the samples by reading the temperature from the display or from DeltaT or esti...

Page 30: ...3 6 Freezing Samples...

Page 31: ...has been repaired by a suitably trained person All parts of this equipment including internal fuses and batteries must only be replaced by a suitably trained person With the exception of fuses and ba...

Page 32: ...filter is not blocked by ice if using an LNP4 pump The Chamber will not Heat Check that the freezer did not overheat wait until the thermal cut out resets No Response from the System Check the mains s...

Page 33: ...paired on site by Planer plc personnel the Safety Declaration described in Appendix C Safety Declaration must be completed Disposal Do not dispose of with general waste Ensure that the system has been...

Page 34: ...4 4 Routine Maintenance and Troubleshooting...

Page 35: ...perating temperature For indoor use only Operating environment 5 to 95 RH non condensing Storage humidity 10 C to 50 C Storage temperature Environment 11 500 ml blood bags 11 250 ml blood bags 22 50 m...

Page 36: ...been cleared F1 T 1A L 250V IEC 127 F2 T 5A L 250V IEC 127 Batteries Batteries must only be replaced by suitably trained service personnel This product contains a lithium battery which is an explosion...

Page 37: ...ed as the sole alarm or for safety critical applications Do not exceed the maximum rating of the relay output stated below The user must ensure that the equipment attached complies with all regulatory...

Page 38: ...B 2 Alarm Output...

Page 39: ...SS recommendations support the issue of a certificate statement of decontamination prior to submitting the equipment for servicing The declaration overleaf must be completed signed by a competent auth...

Page 40: ...r agreement of the recipient whose reference or contact name must be given above 1 Has this equipment item been exposed internally or externally to hazardous materials as indicated below Provide furth...

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