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31 

 

       

 

   

Filling water reservoir

 

 

To fill the water reservoir, do the following:   

 

1.

 Open the outer door and remove the lowest tray. 

See Fig.1. 

 

2.

 Remove a screw in front side and rotate the water 

supply inlet cover to open the water supply inlet. 
See Fig.2.   

 
3.

 When there is no water in the water reservoir (for 

example, first time to operate), add approximately 20 
L of preheated sterile distilled water at 37

 o

C. When 

the water level alarm lamp (RH PAN) is blinking, add 
approximately 15 L of preheated sterile distilled 
water at 37

 

o

C. See Fig.3. It might take 

approximately 2 minutes until the lamp goes off. 

 
4.

 Make sure the water level alarm lamp (RH PAN) 

on the control panel does not blink.   

 
5.

 Rotate back the water supply inlet cover and 

tighten the removed screw. 

 

6.

 Put the lowest tray back in place and close the 

outer door.   

 
Note: 

• Add sterile distilled water as water for humidifying. 
The ion exchange water is not fit to use. 
• The sterile water filled in the water reservoir should 
be pre-heated at 37 

o

C.  Cold water lowers the 

chamber temperature and humidity. 
 
 
 
 
 
 
 
 
 
 
 
 

Fig. 1 

Fig. 2 

Fig. 3 

Water supply inlet

Summary of Contents for MCO-80ICL

Page 1: ...arefully before using this product and keep the operating instructions for future use See page 39 for model number Operating Instructions CO2 Incubator This product is for research use only Not for cl...

Page 2: ......

Page 3: ...CAUTIONS FOR CULTURE 20 START UP OF UNIT 21 SETTING OF CHAMBER TEMPERATURE AND CO2 DENSITY 22 KEY LOCK FUNCTION 25 ALARMS SAFETY FUNCTIONS 26 SETTING OF ALARM RESUME TIME 28 Operation after power fai...

Page 4: ...ent of performance or functions Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect Contact our sales representative or agent if...

Page 5: ...lure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property Symbol shows This symbol means caution This symbol means an action is prohibited This s...

Page 6: ...vec mercure For more information on safe handling procedures the measures to be taken in case of accidental breakage and safe disposal options visit ec gc ca mercure mercury Dispose of or recycle in a...

Page 7: ...t is not grounded it will be necessary to install a ground by qualified engineers Never ground the unit through a gas pipe water main telephone line or lightning rod Such grounding may cause electric...

Page 8: ...may cause electric shock or fire When removing the power supply plug from the power supply outlet grip the power supply plug not the cord Pulling the cord may result in electric shock or fire by shor...

Page 9: ...of setting Be careful not to tip over the unit during movement to prevent damage or injury Prepare a safety check sheet copy the last page when you request any repair or maintenance for the safety of...

Page 10: ...C Maximum relative humidity 80 for temperature up to 31 o C decreasing linearly to 50 relative humidity at 40 o C Mains supply voltage fluctuations up to 10 of the nominal voltage Transient overvoltag...

Page 11: ...INCUBATOR COMPONENTS Rear upper side Bottom of chamber Upper left side When the bottom cover is removed 1 2 3 4 5 6 inner face of ceiling 7 8 9 10 12 11 13 14 15 16 17 18 19 20 21 22 24 25 23 27 26 2...

Page 12: ...roperly At the first time to operate press the reset button between two inside power outlets 11 Access port When not in use cap them with two attached silicon caps on outside 12 Connecting port for CO...

Page 13: ...r reservoir Detects the water level in the water reservoir 26 Auto water supply inlet When an optional component MCO 80AS auto water supply system is installed water is supplied from here 27 UV lamp o...

Page 14: ...lights when the outer door is open 8 Water level alarm lamp RH PAN This lamp blinks when the water in the water reservoir is less than approximately 5 L 9 Over heat lamp OVER HEAT This lamp lights whe...

Page 15: ...in the controller 18 Numerical value shift key Pressing this key in the setting mode causes the numerical value to shift In key lock mode pressing this key makes key lock ON or OFF 19 Digit shift key...

Page 16: ...n between COM to N C Close between COM to N O Contact capacity DC 30 V 2 A Note When the power switch is OFF or the power failure condition the contact output between COM to N O is CLOSE the contact o...

Page 17: ...ll the unit in stable condition to avoid the vibration or noise Unstable condition may cause vibration or noise WARNING Install the unit on a sturdy floor If the floor is not strong enough or the inst...

Page 18: ...unterclockwise to contact them to the floor Ensure the unit is level 3 Fix the unit Two fixtures are attached to the rear of the frame Fix the frame to the wall with these hooks and rope or chain 4 Gr...

Page 19: ...nect the gas regulator to the connecting port for CO2 gas pipe located at the rear upper side of the CO2 incubator 3 Set the CO2 pressure on the secondary side to 0 1 MPa G 1 0 kgf cm2 G 14 5 psi G at...

Page 20: ...in the chamber For this reason avoid using equipment in the chamber that take power source from any other than the outlet equipped in the product since it may be dangerous If there is too much heat em...

Page 21: ...ially when the culture medium is spilled clean and disinfect the chamber immediately Refer to page 29 Routine maintenance for details Keep the inside panels dry To protect the inside of the unit from...

Page 22: ...ture distribution and CO2 concentration in the chamber Stored materials Never place acid or alkaline materials or materials that release corrosive gas in the chamber Such materials can cause failure r...

Page 23: ...the chamber and internal attachments Refer to Sterilizing of chamber and attachment on page 29 3 Place the drain tank under the unit Insert a drain outlet into the hole of drain tank 4 Fill the water...

Page 24: ...digit shift key and numerical value shift key set the figure to 05 0 When pressed the changeable digit is shifted When pressed the figure of settable digit changes 6 Press enter key ENT Set CO2 densi...

Page 25: ...s enter key ENT Set value is memorized The current chamber temperature is displayed Table 2 No humidifying mode setting procedure Description of operation Key operated Indication after operation 1 The...

Page 26: ...he unit is under normal conditions The unit enters calibration mode by pressing this key Wrong key operation may affect the basic performance When the unit enters calibration mode by mistake wait for...

Page 27: ...g change from key lock OFF to key lock ON Description of operation Key operated Indication after operation 1 The current chamber temperature and CO2 density are displayed 2 Press digit shift key for 5...

Page 28: ...ternately with the temperature on the digital temperature indicator Gas supply line indicator blinks Intermittent tone Gas supply line is altered Remote alarm Chamber temperature sensor abnormality If...

Page 29: ...displayed alternately with the temperature on the digital temperature indicator Intermittent tone Remote alarm Glass frame heater abnormality If the glass frame heater or the glass frame heater relay...

Page 30: ...tal CO2 density indicator The middle digit blinks 5 Set the figure to 020 with the numerical value shift key When pressed the figure of settable digit changes 6 Press enter key ENT The setting is memo...

Page 31: ...ff lightly Sterilizing of chamber and attachments When the chamber of the unit is contaminated the chamber and internal attachments should be cleaned and sterilized as follows Note Take care not to da...

Page 32: ...water reservoir with a gauze containing alcohol for sterilization and then wipe off with a dry gauze 10 Wipe the water level sensor with a gauze containing alcohol for sterilization and then wipe off...

Page 33: ...blinking add approximately 15 L of preheated sterile distilled water at 37 o C See Fig 3 It might take approximately 2 minutes until the lamp goes off 4 Make sure the water level alarm lamp RH PAN on...

Page 34: ...gital CO2 density indicator goes out 3 Adjust the set value to the actual value of 36 8o C with the digit shift key and numerical value shift key then press enter key ENT 4 The unit automatically reve...

Page 35: ...en press the enter key ENT 6 The unit automatically reverts to the display mode Note This calibration is available when the setting of CO2 density is 2 or more Example For an internal CO2 density of 5...

Page 36: ...as in the cylinder Replace the cylinder soon While an equipment are operated in chamber Generation of heat by the equipment raises temperature in chamber If the chamber temperature is not equal to the...

Page 37: ...sales representative or agent DISPOSAL OF UNIT WARNING If the unit is to be stored unused in an unsupervised area for an extended period ensure that children do not have access and doors cannot be clo...

Page 38: ...line switching key 5 Select a CO2 gas supply line A or B 6 When one cylinder is empty the indicator blinks buzzer sounds and E01 and current chamber temperature are displayed alternately on the digit...

Page 39: ...voir decreases less than approximately 5 L water is supplied little by little to prevent the water temperature from dropping rapidly The water supply valve is opened and closed repeatedly Automatic wa...

Page 40: ...sted Air filter 0 3 m Efficiency 99 97 or more Water level sensor Thermal type Alarm High Low temperature alarm CO2 density alarm Upper limit temperature alarm Door alarm Remote alarm contact Allowabl...

Page 41: ...2 control range 0 to 20 CO2 variation 0 15 ambient temperature 25o C setting 37o C 5 no load Chamber humidity Normal mode Over 80 R H High humidity mode Over 90 R H Maximum heat emission 6307 kJ h Usa...

Page 42: ...of the unit Unit interior No contamination Decontaminated Contaminated Others 3 Instructions for safe repair maintenance disposal of the unit a The unit is safe to work on b There is some danger see b...

Page 43: ......

Page 44: ...Printed in Japan LDCL041600 0 S0418 0 1 1 1 Sakada Oizumi machi Ora gun Gunma 370 0596 Japan PHC Corporation 2018...

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