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Parker Hannifin

Precision Fluidics Division

45 Route 46 East

Pine Brook, NJ 07058

973-575-4844

http://www.parker.com/ppf

PICOSPRITZER

®

III Manual

Pressure Systems for Ejection of

Picoliter Volumes in Cell Research

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Summary of Contents for PICOSPRITZER III

Page 1: ...er Hannifin Precision Fluidics Division 45 Route 46 East Pine Brook NJ 07058 973 575 4844 http www parker com ppf PICOSPRITZER III Manual Pressure Systems for Ejection of Picoliter Volumes in Cell Research ...

Page 2: ...nspections of the connections in the pressure system should be made to ensure they are all still tight particularly after a period of inactivity or if the system is moved This instrument is intended for use in an indoor environment between 10 and 40 degrees Celsius 50 to 104 degrees Fahrenheit with no more than 90 humidity non condensing EQUIPMENT SPECIFICATIONS Power Requirements 100 to 240 Volts...

Page 3: ... front panel push button external stimulator 5 volt or remote push button foot pedal The Picospritzer III pressure system was designed for rapid and reproducible ejections of picoliter to nanoliter volumes used in conjunction with intracellular or extracellular studies while avoiding the inherent desensitization of nerve cells which accompanies Iontophoretic methodology Intracellular applications ...

Page 4: ...on it PLEASE NOTE An important detail in arranging the location of the solenoid in relation to the pipette holder is to maintain a loose coil in the interconnecting small bore 1 16 inch Teflon tubing to absorb or dampen the pressure pulse thus avoiding movement of the pressure pipette DO NOT stretch the tubing out tight between the valve housing and the pipette holder II OPERATING SPECIFICATIONS S...

Page 5: ... held down It also restricts the interval between repetitive pulses initiated by the push button to approximately 200 milliseconds The PULSE indicator will light during the course of the pulse EXTENDED RANGE The basic Picospritzer is furnished with extended range timing controlled by a three position toggle switch located at the right side of the Duration thumbwheel The top position labeled MSEC s...

Page 6: ...he duration of the pulse with respect to a biological signal much like that of the artifact associated with iontophoresis It is a 5 volt TTL signal controlled by channel one The time mark not only provides a useful indication of the duration of the pulse but it may also serve as a sync out signal for triggering the sweep of an oscilloscope etc HOLDERS FOR PRESSURE PIPETTES Standard pipette holders...

Page 7: ...ars as precursors of glycoproteins 12 in order to study neuron specific transmitter biosynthesis axonal transport and cellular topography The reproducible and quantifiable ejections obtained with pressure systems make them ideal for neuropharmacological studies of agonist and drug interactions with membrane receptors 1 3 4 As you find additional uses for your Picospritzer please send us a reprint ...

Page 8: ...tylcholine by identified neurons of Aplysia Proc Nat Acad Sci 70 3371 1973 12 Thompson E B Schwartz J H and Kandel E R A radio autographic analysis in the light and electron microscope of identified Aplysia neurons and their processes after intrasomatic injection of L H3 Fucose Brain Research 112 251 1976 13 Baux G Simonneau M Tauc L Transmitter release Ruthenium red used to demonstrate a possible...

Page 9: ...d FIGURE 2A LINEARITY OF VOLUME EJECTED WITH VAYRING PULSE DURATION AT CONSTANT PRESSURE The X intercept 15 MSEC represents the mechanical lag time of the particular solenoid valve FIGURE 2B LINEARITY OF VOLUME EJECTED WITH VARYING PRESSURE AT CONSTANT PULSE DURATION The X intercept 7 5PSI represents the minimum pressure necessary for ejection and is a characteristic of a particular pipette FIGURE...

Page 10: ...onse standard error of the mean Problems of desensitization can be circumvented with a pressure pipette since the pipette is usually filled with agonists in the concentrations of 10 6 to 10 3 M in contrast to iontophoretic pipettes Braking current is not necessary for the pressure pipette thus avoiding inconsistent ejection of compounds FIGURE 3C Reproducibility of 12 consecutive responses to acet...

Page 11: ...ch OD tubing 3 feet long nut ferrule on both ends REPLACEMENT GASKETS P N 050 00XX GSK KIT XX should be replaced by your Pipette s OD Example P N 050 0010 GSK KIT is for a 1 0 mm pipette holder Each Kit comes with 10 replacement gaskets and 1 replacement nut PICOSPRITZER ACCESSORIES Pushbutton Cable Assembly P N 050 0000 800 1 Footswitch Cable Assembly P N 050 0000 801 1 PIPETTE HOLDERS P N 050 00...

Page 12: ...the tubing required to connect it to the front panel mounted regulator assembly and to a pipette holder 12 feet of inch OD tubing are supplied to make the connection between the regulator assembly and the compressed gas supply maximum 150 psig Expansion capability for a second valve box is built in B Two Channel This PICOSPRITZER uses the same electrical chassis as the single channel but includes ...

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