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23

10.

Draining

Draining can be performed in a number of ways according to the specific application requirements. 
Table 1 below gives drainage approaches according to application requirements.

Before draining the mixer, follow the below instructions:

1. Connect the outlet port to the desired downstream equipment or receptacle according to the specific

fitting on the single-use system (e.g. Kleenpak sterile connector or quick connector).

2. Open the outlet port valve by gently rotating the flush port bayonet valve to a stop, allowing fluid to

flow out of the Allegro mixer biocontainer.

CAUTION!

Take care to not over-rotate, apply excessive force, or pull the flush outlet valve during 

operation. This may cause it to detach from the mixer. Use MINIMUM force when rotating the bayonet
valve to open the outlet line.

Table 1

Draining the mixer system

Mixer rotation

‘Bag Inflation’ Setting

When to Use

Comments

On

ON 

To keep the mixer inflated 

Once the mixer is empty, ‘Bag Inflation’ 

Then OFF

Then VENT

and to continue mixing 

can be switched to ‘Vent’ to allow 

during dispensing the 

collapse of the bag prior to removal. 

mixed volume

Once ‘Vent’ has been selected, the top 
of the bag should be gently pushed down 
into the mixer tote to free the top hat 
from the upper gasket to vent the 
biocontainer and allow removal.

OFF

ON (until fluid dispensed)

To keep the mixer inflated 

As above

Then VENT

during dispensing the mixed 

No agitation of fluid during dispensing

volume

OFF

OFF

To allow the mixer to collapse 

The top of the mixer SU system should 

during dispensing the mixed 

be gently pushed down into the mixer 

volume

tote to free the top hat from the upper 
gasket at the start of the fluid dispensing 
phase. Allows for removal of the single-
use mixer system as soon as fluid 
volume is fully dispensed from the system. 

Note: Fluid dispensing is normally done using a peristaltic pump on the outlet tubing, especially when a filter is being used down-
stream of the mixer chamber. 

The floor of the Perspex tote is specifically designed to 
provide very high yield/fluid recovery during draining.

Summary of Contents for Allegro 50 L

Page 1: ...USD2915a Instructions For Use Allegro 50 L Single Use Mixer ...

Page 2: ...m the Packaging 12 6 2 Installing the System 14 6 3 Connecting Sensors 18 6 3 1 Non Aseptic Port Sensor Attachment 18 6 3 2 Aseptic Port Sensor Attachment Connecting to a Female Kleenpak Sterile Connector 19 7 Fluid Addition 20 8 Running the Allegro Mixer 21 9 Adding Powder 22 10 Draining 23 11 Single Use System Removal 24 12 Troubleshooting 24 13 Specifications 25 14 General Maintenance 26 15 WEE...

Page 3: ...he exterior of the biocontainers may in circumstances where significant stress repetitive bending and twisting is applied during use cause damage to the molded LDPE inlet and outlet ports The flexible LDPE film of the Allegro 3D biocontainers is not affected This mixer must only be used by persons who have been trained and authorized to do so 3 Receipt of Equipment 3 1 Allegro Mixer Hardware Take ...

Page 4: ... systems used in the Allegro mixer range are designed for single use only 4 Pictures and Schematics 4 1 Mixer Tote Pictures Figure 1 Standard Allegro 50 L mixer with plastic tote Figure 2 Standard Allegro 50 L mixer jacketed tote as an alternative to the plastic tote Acrylic Perspex Tote Powder Bag tubing Support Frame optional Plastic Tote Door Sensor Support Bracket Motor and Gear Box 4 Sided Ja...

Page 5: ... Cabinet Vent 225 mm pH Probe Electrical Power Isolation Switch and Indicator Light in Hose Barb Gas Exhaust Port Blanking Plate Used for Optional Transmitters Interlock LEDs Mixer Operation Ready LED Emergency Stop in Hose Barb Gas Inflation Port Mixer Bag Pressure Gauge Impeller Speed and Direction Controls Variable Speed Drive VSD Inflation and Vent Control ...

Page 6: ...ed PSU Polysulfone in Fluid Inlet Port in Gas Exhaust Port 3 in Powder Addition Port in Fluid Inlet Ports 2 e g acid base in Powder Addition Port Mixer Biocontainer PSU Low Volume Mixer Impeller in Gas Inflation Port in Sensor Port in Sample Port in Sensor Ports 2 Probe Manifold Clip Drive Coupling PSU in Flush Port Outlet PSU Low Volume Mixer Impeller ...

Page 7: ... Bag Vent using optional 12 mm pneumatic hose if collecting exhaust nitrogen 4 BAG VENT 2 Upstream Bag Vent using optional 12 mm pneumatic hose if collecting exhaust nitrogen Optional Connections Figure 6 Optional sensor and comms connections UCI INNER FACE FRONT PORT 1 PORT 2 Used for connecting appropriate sensor cables at manufacture depending on mixer configuration PORT 3 PORT 4 Used for conne...

Page 8: ... the other end to the Allegro MVP system allows the impeller to be started and stopped the speed adjusted and the motor direction to be reversed remotely For further information on how to operate these functions please refer to the instructions for use of the Allegro MVP system Note If the remote control cable is removed the port plug must be re attached to enable normal operation from the UCI key...

Page 9: ... of heat transfer fluid The heat transfer fluid flow rate is adjusted according to the pressure ratings of the Allegro jacketed mixer tote Typical pressure drop is shown above CAUTION Ensure the feed and return lines are isolated and depressurized prior to removal The heat transfer fluid is drained through the jacketed tote drain valve following the operation using the low point drain for heating ...

Page 10: ...m is shipped with the weight of the tote lifted off the load cells by means of 4 half nuts that are rotated upwards on transit bolts This protects the load cells from shock and vibration during transit The 4 nuts must be wound down the transit bolts to allow the tote to sit freely on the load cells before any measurements are attempted The transit bolts must not be removed full perform ance is obt...

Page 11: ...any liquids or solids added after this point will be displayed Likewise if the user wishes to only weigh a proportion of the compounds being added pressing the tare key again just before that addition will allow this setting the display back to zero The tare button can be pressed multiple times each time removing the last added weight from the display in readiness for the next to be added and meas...

Page 12: ...11 If the weigh platform will not zero check the wheel locks are on and the system is stable In certain instances you may need to Set Zero by following the steps indicated below ...

Page 13: ...ng These can damage the single use system Inspect system for damage Do not use if damaged Allegro single use mixer systems are typically double bagged Remove system carefully from packaging www pall com biopharm 12 1 Check packaging is undamaged prior to opening 2 Open the box and discard the protective packaging according to local regulations ...

Page 14: ... down the sides to reveal the inner packaging bag Remove outer bag 5 Use the easy tear to open up side of the SUS inner bag 6 Pull the top face of the outer bag to tear down the sides to reveal the single use mixer system Remove outer bag 7 Carefully turn the system over to expose the drive shaft coupling ...

Page 15: ... power and check that the power light comes on WARNING Power must be ON to control bag pressure during operation 1 Apply wheel locks to the mixer tote 3 Open the door to the Allegro 50 L mixer tote 4 Push the white section at the top of the single use system up through the hole in the lid of the plastic or jacketed tote ...

Page 16: ...light is illuminated 7 Connect the inflation and exhaust lines from the single use system to the hose barbs on the User Control Interface UCI WARNING Inflation and exhaust lines MUST be connected to appropriate ports and be free from blockage or restriction to ensure proper regulation of air pressure during operation ...

Page 17: ...9 As the system inflates the shaft coupling will move towards the bottom of the tote Align the shaft coupling sample line and outlet tubing with the ports on the bottom of the tote see next picture 10 As the system approaches full inflation STOP inflation by turning the BAG INFLATION switch to the OFF position to allow operator to conduct steps 11 12 Drive coupling port Outlet tubing port Outlet t...

Page 18: ... the shaft coupling is correctly located the 2 SHAFT light is illuminated on the UCI If the light is not on refer to troubleshooting SHAFT interlock pin which will activate the UCI light when the SUS is in the correct position 12 Close and secure the door and check DOOR interlock light is illuminated 13 Turn the BAG INFLATION switch to the ON position ...

Page 19: ...Check all lights are on and the variable speed drive is energized shows STOP on the display 1 Turn the BAG INFLATION switch to the OFF position 2 Unscrew the plug from the non aseptic sensor port on the Allegro mixer biocontainer 3 Insert appropriate sensor e g conductivity into the sensor port and screw in to hand tight Note this should be sufficient to create a fluid seal during operation typica...

Page 20: ...attached to the single use system and the male Kleenpak sterile connector on the bellows assembly and follow the Kleenpak sterile connector instructions Pall document reference number USD 2233 to make the sensor connection 2 Connect the male and female Kleenpak sterile connectors peel strips still in folded position strips facing up by clicking them together inspecting the locating regions on both...

Page 21: ...r by pushing the sensor into the mixer biocontainer collapsing the bellows 7 Engage the end of the bellows in to the sensor support bracket to maintain optimum sensor position in the mixer 1 Connect a suitable fluid addition line to the mixer inlet line and add fluid WARNING Filling phase must be observed to ensure pressure does not exceed specifications as outlined below Approximate fill volume c...

Page 22: ...eration Accurate fill volume by weight measurement can be determined using the weigh platform version of the 50 L mixer Here the mixer trolley is equipped with built in load cells To set the speed for the rotation use the panel to increase the figure to the desired value The direction of rotation is set as either FWD or REV FWD Clockwise Up flow REV Counter clockwise Downflow To change from curren...

Page 23: ...port on the mixer A When connected the red clip B on the powder bag can be removed to dispense the powder into the fluid Some powder bags have a flush line WARNING Do not attempt to place hand through the 3 in powder addition port at any point during operation Risk of personal injury 5 Reconnect 3 in powder port cap secure with clamp and switch inflation to ON to re inflate the mixer biocontainer ...

Page 24: ...ce the mixer is empty Bag Inflation Then OFF Then VENT and to continue mixing can be switched to Vent to allow during dispensing the collapse of the bag prior to removal mixed volume Once Vent has been selected the top of the bag should be gently pushed down into the mixer tote to free the top hat from the upper gasket to vent the biocontainer and allow removal OFF ON until fluid dispensed To keep...

Page 25: ...ating correctly by carefully switch operating it manually to check that it is working Check the cable connection to the micro switch Replace with spare item or call Pall for warranty servicing At the end of Inflation process System is not powered up Check system is connected correctly to power supply and the the 2 SHAFT interlock LED is LED on the UCI power light is illuminated GREEN NOT illuminat...

Page 26: ...support mm in Materials of Construction Tote Acrylic Perspex 304 Stainless Steel Acrylic Perspex 304 Stainless Steel Frame 304 Stainless Steel 304 Stainless Steel 304 Stainless Steel 304 Stainless Steel Wheels Nylon Stainless Steel Nylon Stainless Steel Nylon Stainless Steel Nylon Stainless Steel Weighing Platform N A N A 20 20 Accuracy g Gas connections Supply Cabinet Vent 10 mm Pneumatic tubing ...

Page 27: ...al the following critical spares should be considered to be held in stock since failure of these items in use will prevent use of the mixing system Component replacement instructions can be obtained from Pall Advanced Separations Systems PASS engineering group Please contact Pall To isolate the mixer before disconnection or maintenance work Switch off electrical power supply at the main isolating ...

Page 28: ... that Allegro hardware and systems manufactured by Pall when properly stored and installed and operated at ratings specifications and design conditions will be free from defects in material and workmanship during their shelf life Pall liability under any warranty is limited solely to replacing or issuing credit for the Allegro systems and hardware that may become defective during the Warranty Peri...

Page 29: ...t the single use system when controlled collapsing the mixer system prior to removal from the system Switch in FILL position Use this position to control the inflation of the mixer bag during normal operation Switch in OFF position Use this position when adding powder to the mixer via the powder port or when removing the bag from the system following use ...

Page 30: ...29 20 Appendix 2 Wiring Diagrams 21 Appendix 3 General Assembly Drawings ...

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Page 36: ...calibration buffers for a range of pH values Conditioning pH electrodes are shipped with the electrodes moist Prior to using your electrode for the first time follow these three steps to condition your electrode 1 Remove the protective cap or rubber boot from the bottom of the sensor and rinse the electrode with distilled or deionized water 2 Place the electrode in a beaker containing one of the l...

Page 37: ...cover the glass bulb and replenish as needed to keep the bulb moist From http www coleparmer com TechLibraryArticle 550 Corporate Headquarters Port Washington NY USA 1 800 717 7255 toll free USA 1 516 484 5400 phone biopharm pall com e mail European Headquarters Fribourg Switzerland 41 0 26 350 53 00 phone LifeSciences EU pall com e mail Asia Pacific Headquarters Singapore 65 6389 6500 phone sgcus...

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