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version 1.5 rev 4 Dec 2019

7

Sona & 

Marana

s

afety

 

and

 W

aRning

 i

nfoRMation

READ THIS INFORMATION FIRST

  

1.  If the equipment is used in a manner not specified by Andor, the protection provided by the equipment may be 

impaired.

2.  Before using the system, please follow and adhere to all warnings, safety, manual handling and operating 

instructions located either on the product or in this Hardware Guide.

3.  Users must be authorised and trained personnel only; otherwise this may result in personal injury, and/ or 

equipment damage and impaired system performance.

4.  There are no user-serviceable parts inside the product and the enclosure must not be opened. Only authorised 

service personnel may service this equipment.

5.  Do not position this product so that it is difficult to operate the Mains disconnecting device. See SECTION 4.1, 

“Emergency Mains Disconnection”.

6.  Protective earth is an integral part of the protection against electric shock in this product, and is provided via 

the earth pin of the external power supply. Ensure that this is plugged into the building earth system via the 
mains socket. Do not tamper with any of the earthing measures.

7.  Only the correctly specified mains supply should be used.

8.  Only the AC/DC external power supply provided with the product should be used.

9.  Only the power supply cord provided with the product should be used. Should this not be correct for your 

geographical area, contact your local Andor representative.

10.  Make sure the power supply cord is located so that it will not be subject to damage. If replacement of the  

detachable power supply cord is required, ensure replacement is of same type and rating.

11.  Performance of the system may be adversely affected by rapidly changing environmental conditions or 

operation outside of the operating conditions specified in “TECHNICAL SPECIFICATIONS”

12.  While running an experiment, try to keep temperature as stable as possible.

13.  This equipment has not been designed and manufactured for the medical diagnosis of patients.

14.  Electromagnetic Compatibility: This is a Class B product.

15.  This product has been designed and tested to perform successfully in a normal (basic) electromagnetic 

environment, e.g. a typical life science test laboratory, as per the EU EMC Directive. It is not designed to 
operate in a harsh electromagnetic environment, e.g. close to the following equipment: EMI/RFI generators, 
electrostatic field generators, electromagnetic or radioactive devices, plasma sources, arc welders, x-ray 
instruments, intense pulsed sources, or other similar sources of high energy fields whose emissions are not 
within the normal range expected under the EU EMC Directive.

16.  Please note that this product is not designed to provide protection from ionising radiation. Any customer using 

this product in such an application should provide their own protection.

17.  Your product is a precision scientific instrument containing fragile components.  Always handle it with care.

18.  Do not wet or spill liquids on the product, and do not store or place liquids on the product.

19.  If spillage occurs on the product, switch off power immediately, and wipe off with a dry, lint-free cloth.

Summary of Contents for Andor Sona 2.0B-11

Page 1: ...Sona Marana andor com Andor Technology Ltd 2019 Version 1 5 rev 4 Dec 2019 Hardware Guide for Sona 4 2B 11 2 0B 11 and Marana 4 2B 11 Models ...

Page 2: ...ECTION 3 INSTALLATION 18 3 1 TRANSPORT AND STORAGE INFORMATION 18 3 2 USING THE LENS MOUNT 18 3 2 1 C Mount Adaptor 18 3 2 2 F mount and T mount Adaptors 19 3 3 USING A MAGNIFYING COUPLER UNIT MCU 19 3 4 MOUNTING THE CAMERA USING THE MOUNTING FLANGE 19 3 5 CONNECTING THE CAMERA TO THE PC 20 3 5 1 Installing the USB 3 Card in the PC if required 20 3 5 2 Connecting the Camera to the PCIe Card 21 3 6...

Page 3: ... 27 4 4 1 sCMOS Sensors 27 4 4 2 Extended Dynamic Range EDR 28 4 4 3 Using ROIs AOIs 29 4 4 4 Binning 29 4 4 5 Spurious Noise Filter 29 4 4 6 Blemish Correction Filter 30 4 5 TRIGGER MODES 30 4 6 SOFTWARE ACQUISITION EVENTS 31 4 7 ROLLING SHUTTER 31 4 7 1 Rolling Shutter Mechanism 32 4 7 2 Signal Information 32 4 7 3 Timing Parameters and External Triggering 33 4 7 4 Rolling Shutter Internal Trigg...

Page 4: ...ETY CHECKS 41 5 3 GENERAL CLEANING DECONTAMINATION INFORMATION 41 5 4 CLEANING THE CAMERA WINDOW 41 5 4 1 Tools Required 42 5 4 2 Window Cleaning Procedure 42 5 5 FUSE REPLACEMENT 42 5 6 COOLING HOSES AND CONNECTIONS 42 SECTION 6 TROUBLESHOOTING 43 6 1 PREVENTING CONDENSATION 43 6 2 QUICK TROUBLESHOOTING GUIDE 44 ...

Page 5: ... 1 5 rev 4 Dec 2019 5 Sona Marana APPENDIX A TECHNICAL SPECIFICATIONS 45 APPENDIX B MECHANICAL DRAWINGS 48 APPENDIX C DEWPOINT INFORMATION 49 APPENDIX D REFERENCE INFORMATION 50 APPENDIX E OTHER INFORMATION 51 ...

Page 6: ...ted China office address table on page 39 and power supply information 1 4 1 Aug 2019 Updated power supply information 1 5 4 Dec 2019 Updated accessories table and model applicability of hardware guide Updates to the Manual Changes are periodically made to the product and these will be incorporated into new editions of the manual Please check for new releases of the manual in MyAndor http my andor...

Page 7: ...ly cord is located so that it will not be subject to damage If replacement of the detachable power supply cord is required ensure replacement is of same type and rating 11 Performance of the system may be adversely affected by rapidly changing environmental conditions or operation outside of the operating conditions specified in TECHNICAL SPECIFICATIONS 12 While running an experiment try to keep t...

Page 8: ...eaks that may find their way to the mains electricity supply and create a hazard Refer to the information in SECTION 3 7 for general guidelines on the correct installation and use of a liquid cooling system 22 See SECTION 5 1 Cleaning and Decontamination 23 Do not expose the product to extreme hot or cold temperatures 24 Do not expose the product to open flames 25 Do not allow objects to fall on t...

Page 9: ...etain the packaging materials to transport or return equipment if required If the equipment appears damaged in any way return it to sales outlet in its original packaging No responsibility for damage arising from the use of non approved packaging will be accepted Ensure all items and accessories specified at the time of ordering and as detailed on the packing list are present if any items are miss...

Page 10: ...na for physical science imaging such as space debris tracking and occultations This manual includes a description of the main features of the cameras their installation routine operation and troubleshooting It also provides a summary of some of the technical features of the Sona and Marana back illuminated sCMOS camera series For further information on operation and control of the camera refer to ...

Page 11: ...92 Andor Technology 300 Baker Avenue Suite 150 Concord MA 01742 USA Tel 1 860 290 9211 Fax 1 860 290 9566 Asia Pacific China Andor Technology Japan 5F IS Building 3 32 42 Higashi Shinagawa Tokyo 140 0002 Japan Tel 81 3 6732 8968 Fax 81 3 6732 8939 Andor Technology China Haitong Times Business Center Building B2 West No 11 West Third Ring North Road Haidian District Beijing 100089 China Tel 86 0 10...

Page 12: ...THE INFORMATION PROVIDED HEREIN 1 3 Copyright and Protective Notices The copyright in this document and the associated drawings are the property of Andor Technology Ltd and all rights are reserved This document and the associated drawings are issued on condition that they are not copied reprinted or reproduced nor their contents disclosed The publication of information in this documentation does n...

Page 13: ...ordered 1 Software Driver CDs or Flash Drive Solis is ordered separately 1 Hardware Guide in electronic format 1 Power Supply 1 x 15 V 1 Power Cord Country specific 2 USB 3 Card 1 Quick Start Guide 1 USB 3 Standard A to Standard B cable 1 Anti static Wrist band 1 Multi timing I O cable 1 Coolant pipes hose inserts pair 1 Performance Sheet 1 ...

Page 14: ...ge of accessories is available see below The Sona and Marana are intended only for use with accessories supplied and recommended by Andor Description Code MCU with 2x magnification for matching Sona to Leica microscopes MCU SONA LEI MCU with x2 magnification for matching Sona to Nikon Ti Series TiE and Ti2 microscopes MCU SONA NIK TI MCU with x2 magnification for matching Sona to Olympus microscop...

Page 15: ... C mount other adapters are available Sensor The Sona and Marana feature high resolution sCMOS 2 0 and 4 2 Megapixel back illuminated sCMOS sensors Andors unique UltraVacTM vacuum technology has been utilised UltraVac provides a permanent hermetically sealed enclosure so that the QE and cooling performance can be maintained over many years operation Camera Window There is a single AR coated UV gra...

Page 16: ... trigger on a rising edge ARM and AUX_OUT_1 FIRE FIRE n and AUX_OUT_2 for 7 way cable only outputs are all TTL timing outputs These can be individually inverted via software e g Solis or SDK AUX_OUT_1 supplies the FIRE ALL output by default This is the logical AND of the FIRE pulses associated with Row 1 and Row n the last row read out in the image frame Therefore the FIRE ALL pulse represents the...

Page 17: ... power connector for the camera is inserted securely The connector is keyed to aid correct orientation A 15 V DC External Power Supply supplies the camera Refer to the Technical Specifications for the External Power Supply requirements NOTES 1 The electrical mains lead should be certified for use in your country and in applicable countries the plug must be fitted with a 240 V 5 A fuse 2 If users u...

Page 18: ...tion 6 1 for further information Storage Storage Temperature 10 C to 50 C If it is to be stored after use at a temperature below the coolant freezing point ensure that all liquid coolant has been expelled from the camera 3 2 Using the Lens mount An F mount lens mounting system is present on the 4 2B 11 models and a C mount is present on the Sona 2 0B 11 model 3 2 1C Mount Adaptor Note C Mount Uses...

Page 19: ...ate 3 3 Using a Magnifying Coupler Unit MCU A magnifying coupler e g Andor MCU or lens tube may be used to match the large area sensor to the objective magnification and the aperture that is available from the microscope These attach to the camera using a T mount style connection described in Section 3 2 2 For further information on the magnifying coupler please refer to the instructions supplied ...

Page 20: ...t Unused filler brackets Gently press the card into the slot PCIe 2 0 Slot x4 recommended 2 Open the PC enclosure to gain access to the expansion slots 3 Locate a suitable PCIe 2 0 or newer slot Card operates in an x1 slot or one with a greater number of channels i e x4 x8 or x16 4 If you are unsure which slot is correct please consult the PC user manual 5 Remove the filler bracket corresponding t...

Page 21: ...ntrol PC 3 6 Connecting the Camera to the Power Supply Connect the mains power cable between the camera power input 15 V DC and the 15 V DC Power Supply Unit Route cables carefully so they do not pose a tripping hazard or at risk of being unplugged Avoid tight bends or strain at the connections Connect the PSU to the mains power supply 15 V DC Camera Mains Supply ...

Page 22: ...n Appendix C for guidance Use of coolant at or below the dew point can result in permanent damage to the camera head due to formation of condensation on internal components Never use damaged split or worn hoses as leaks may find their way to the mains electricity supply and create a hazard The water cooling system of the camera is rated for operation up to 2 bar 200 kPa If your water supply exceed...

Page 23: ...affected by distance between camera head and cooler Recommended coolant water or water glycol mix depending on the ambient environmental temperature during operation De ionized water without additives may be used as the coolant Some mains supply water is heavily mineralized i e Hard which could cause deposits in the water circuit inside the camera This can reduce the flow rate and cooling efficien...

Page 24: ...the camera head ensure they click into place 3 Confirm the hoses are connected securely by applying pressure on the front of the camera body and pulling backwards on each hose 4 Connect the other ends of the coolant hoses to the cooling system refer to the cooling system manual 3 9 2 Disconnecting the Coolant Hoses 1 Press the latch on the camera hose connection away from the hose 2 Hold the latch...

Page 25: ...estart your computer when prompted to successfully complete the installation 6 The shortcut icon for Solis will appear on the desktop on re start 7 The camera is now ready to be connected to a PC laptop and powered on 3 10 3 New Hardware Wizard When the Sona camera is connected to a PC for the first time the New Hardware Wizard screen will appear 1 Select the No not this time only option then clic...

Page 26: ...us Controllers 2 Select the Advanced tab 3 Check the Disable USB 3 0 power management functions checkbox as shown in the screenshot above 3 10 5 Checking Setting BIOS options for PCs not supplied by Andor Enter the BIOS menu when starting PC For Dell workstations press F12 at start up and select System Setup in the One Time Boot Menu For Dell workstations 3 options in the Performance menu of the B...

Page 27: ... start up the camera software e g Solis or SDK Otherwise the SW SDK will not be able to connect to the camera 5 Launch your camera control software e g Solis or SDK3 6 The camera will now start up under control of the software Note It will take some time for the camera to reach the target cooling temperature This is visible in the temperature status bar in Solis Please wait until the camera has re...

Page 28: ...ow noise and maximum well depth to be used simultaneously This delivers a very high linearity of 99 7 across this range allowing for quantitative analyses When combined with the high frame rates and large sensor area this provides a lot of flexibility for how the camera may be used For dynamic images Use 16 bit mode to provide the data range required to capture full range images Frame rates will b...

Page 29: ...fore commonly used for CCD cameras to increase the signal to noise ratio and speed of readout at the expense of lower resolution For further information refer to the technical note CCD Binning However for cameras with sCMOS sensors such as in the Andor Zyla Neo Sona and Marana models the binning process is performed slightly differently Binning is processed by the FPGA after the pixels have been r...

Page 30: ...external intervention External Trigger the camera and software are in a high state of readiness to accept a trigger from an external source Refer to Table X for the minimum pulse width required to guarantee a trigger The external trigger is fed via the External Trigger input on the I O Connector on the camera head Software Trigger works in a similar manner to External Trigger mode whereby the came...

Page 31: ...g shutter readout mechanism is illustrated below Figure 6 Rolling Shutter Operation At the start of an exposure the Reset wave sweeps through the sensor clearing any accumulated charge from the pixels The pixels then start accumulating light induced charge At the end of the exposure the readout wave sweeps through the sensor transferring the charge from each row into the readout node of each pixel...

Page 32: ...because every time the node is reset it does not settle at exactly the same level and hence the actual level must be measured Step 2 and subtracted from the signal level Step 4 to get the real signal 4 7 2 Signal Information The Rolling Shutter signals in the diagrams are as follows FIRE Exposure for Row 1 In Rolling Shutter mode the FIRE output from the camera indicates to the user the exposure t...

Page 33: ...use all actions with the sensor are synchronised to this Row time In External and External Start Triggering Modes the minimum trigger pulse width detected by the camera is Minimum EXT Trigger Width EXT Trig Pulse Width 6 6 ns 4 7 4 Rolling Shutter Internal Triggering Non Overlap Mode Internal Trigger Mode allows the user to configure an exposure time and cycle time For Internal Triggering Non over...

Page 34: ...ggering long non overlap Parameter Minimum Maximum Exposure 1 Row 30 s Cycle Time 1 Frame Rate Exposure 1 Frame 1 Row 44 000 s Note actual minimum Exposure time is 15 μs in 12 bit mode and 9 48 μs in 16 bit mode The exposure time is incremented in 1 Row time steps ...

Page 35: ... exposure is being read out from the sensor When the required exposure time is less than the time it takes to read out a frame Short Exposures the cycle time is always defined by the time taken to read out a frame 1 Row time See figure 9 below When the required exposure time is greater than the time it takes to read out a frame Long Exposures the cycle time is defined by the Exposure time 1 Row ti...

Page 36: ...e trigger event can either be from the EXT Trigger input or sent via software The ARM signal is asserted to indicate it is ready to detect an incoming trigger input Once the trigger event is detected a rolling reset is initiated this effectively begins a new exposure When the exposure period has completed a signal frame read out phase begins Once the frame has been read out completely the Arm sign...

Page 37: ...ec 2019 37 Sona Marana Figure 12 External Triggering long non overlap Parameter Minimum Maximum Exposure 3 Rows 30 s Cycle Time 1 Frame Rate Exposure 1 Frame 1 Row External Start Delay 0 1 Row EXT Trig Pulse Width 6 6 ns ...

Page 38: ...dth defines the exposure time for all rows but is only coincident with the exposure time for Row 1 The exposure for Row 2 will be delayed by one row time relative to Row 1 and so forth The period of the external trigger pulse defines the overall cycle time If the width of the trigger event is less than 3 Rows the falling edge will be missed and a subsequent falling edge will be required to end the...

Page 39: ...f the next Row read period This frame is discarded as it does not contain the correct exposure period Reading out this first frame effectively begins the first exposure When the next positive edge of the external trigger is detected a signal frame read out is initiated at the start of the next Row read period As each row is read out the new exposure for that row begins Figure 14 External Exposure ...

Page 40: ...Rolling Shutter Triggering Modes Exposure Range Max Trigger Jitter Min Trigger Pulse Width Fast Exposure Switching Supported Internal Non Overlap User settable exposure time User settable Cycle Time 1 Row to 30 s P Internal Overlap On User settable exposure time 1 Row to 30 s P External Non Overlap Exposure time user settable Cycle Time controlled via exter nal trigger pulse 3 Rows to 30 s 1 row 6...

Page 41: ...ody with the dampened cloth Do not use abrasive or other detergents to clean the camera Decontamination In the event any product must be returned the customer must complete a decontamination form to declare the equipment os contamination free and safe for Andor employees to work on http www andor com decontamination aspx 5 4 Cleaning the Camera Window At some point it may become necessary to clean...

Page 42: ...t particles onto the camera window 3 With the shutter if present open turn the camera on its side making it easier for particles to fall out of the camera head Face the window toward you at the edge of a table 4 Give the window short blasts of compressed air to work dust particles out of the camera head 5 If there are still particles stuck on the window use an optics brush to very carefully dislod...

Page 43: ...on be detected You may see condensation on the outside of the camera body if the cooling water is at too low a temperature or if the water flow is too high The first signs of condensation will usually be visible around the connectors where the water tubes are attached If this occurs carry out the following actions 1 Switch off the system 2 Wipe the camera with a soft dry cloth NOTE It is likely th...

Page 44: ...rotating and free of obstruction Camera does not cool to the required temperature Ambient temp is above specified operating range Check ambient temp is with specified range Fans may be obstructed Check fans and vents are rotating and free of obstruction Water cooling system not functioning correctly Check cooling system Camera image quality not as expected e g image noise The sensor must cool down...

Page 45: ... MHz 12 bit mode Quantum Efficiency 2 95 max Read Noise e median 1 6 e at any readout rate Sensor operating temperature 3 Air cooled Water liquid cooled 25 C up to 30 C ambient 45 C 16 C water Dark Current Air cooled 25 C Water liquid cooled 45 C 0 7 e pixel s 0 3 e pixel s Active area pixel well depth 85 000 e Dynamic Range 53 000 1 Data Range 16 bit extended dynamic range 12 bit maximum frame ra...

Page 46: ...art and end of acquisition 6 The Sona connects to your control PC using a USB 3 0 connection This may also be referred to as USB 3 1 Gen 1 Andor provide a USB 3 0 card and cable and recommend that these are used to ensure optimum performance USB 3 0 USB 3 1 Gen 1 and USB 3 2 Gen 1x1 are equivalent 7 Optional user switchable C mount accessory available for use with smaller ROI sizes T mount fitting...

Page 47: ...a 0 15 Ω source resistance is 2 7 A min peak and a pulse width of 15 ms min measured at half the peak Safety Certified to an appropriate IEC standard e g IEC 60950 1 and meet the reinforced insulation from mains requirement of IEC 61010 1 Environmental Ensure that the EPS meets the environmental specification of the overall product see above Camera Power Specifications Mains Input for Supplied Ext...

Page 48: ...version 1 5 rev 4 Dec 2019 48 Sona Marana APPENDIX B MECHANICAL DRAWINGS Weight approx 2 7 kg 5 95 lbs Focal Distance pixel 1 1 row 1 column 1 Sona Marana ...

Page 49: ...condensation is forming will be on the coolant connections entering and exiting the camera Use of coolant at or below the dewpoint can result in permanent damage to the camera head due to formation of condensation on internal components It is therefore very important to ensure that coolant temperature is above the dewpoint Further guidelines are provided in Section 6 1 The relationship between Rel...

Page 50: ...Time Between Failure MTBF value is 100 years Outgassing is minimized through assembly in a state of the art facility with a stringent protocol and use of proprietary materials Outgassing is the release of trapped gasses that would otherwise degrade cooling performance and potentially cause sensor failure UltraVac also features a single camera window for the best optical performance For more inform...

Page 51: ...ng warranty conditions will have been made available during the ordering process The current version may be viewed at www andor com pdfs literature Andor_Standard_Warranty pdf Waste Electronic and Electrical Equipment Regulations 2006 WEEE The company s statement on the disposal of WEEE can be found in the Terms and Conditions found on the Andor website WEEE Policy ...

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