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MiPNet19.01 

B: OroboPOS service

 

23

 

 

 

OROBOROS INSTRUMENTS

 

 

O2k-Core Manual

 

B

• 

Figure  4

.  Mounting  of  a  membrane  onto  the  POS, 

using the membrane mounting tool which consists of 

two  parts,  the  membrane  guide  and  the  membrane 
ring holder. 

No  gas  bubbles 

should be trapped 
in  the  electrolyte 

reservoir 

after 

membrane 

application.  As  a 
check,  you  may 

inspect 

the 

electrolyte 

reservoir  under  a 
binocular. 

No 

folds  should  be 

visible 

in 

the 

membrane  in  the 

central 

area. 

Small folds in the 

membrane  near 
the 

outer 

circumference 

have  no  influence,  but  large  folds  should  be  avoided.  Wash  excess 

electrolyte off the outside of the sensor and  POS connector and wipe dry, 
before attaching the POS connector to the POS holder at the O2k-Main Unit.  

 

 

After  sensor  service  and  membrane  mounting,  the  POS  needs  some 

time  in  operation  to  stabilize  while  the  O2k  is  switched  on.  Such 
stabilization  may  require  several  hours.  For  this  purpose  the  O2k  may  be 

left on overnight. 
 

4.

 

Electrical cable connection 

 

Connect  the  POS  cable  to  the  O2k-Main  Unit, 
avoiding  bending  and  torsions  of  the  cable.  

Insert  the  male  plug  of  the  POS  cable  into  the 
female connector next to the control light of the 
stirrer.  The red dot on the male plug has to face 

accurately upwards when inserting the plug.  

 

See Supplement for O2k Series B to C.

 

 

5.

 

Storage of the OroboPOS

 

 

5.1. Short-term storage in the Oxygraph-2k

 

 

For  short  periods  of  days  or  several  weeks,  the  POS  is  stored  in  the 
Oxygraph-2k  chamber.  The  chamber  is  washed  with  distilled  water  and 

completely filled with 70% ethanol for chemical sterilization. The stopper 
is  inserted  loosely  without  pushing  it  down  beyond  the  point  where  the 

sealing ring is inserted into the glass chamber. This ensures a longer life 
time of the sealing rings. The receptacle of the stopper is completely filled 

with ethanol  from the top,  and is sealed with a  black cover slip  to avoid 

Summary of Contents for Oxygraph-2k

Page 1: ...s of operation Performance of the OROBOROS Oxygraph 2k according to instrumental specifications is obtained only with oxygen sensors which are maintained in a good functional state POS service is required if 1 a new sensor is prepared 2 the raw signal at air calibration declines 3 signal noise is high 4 the time response is prolonged time constant 10 s and biphasic 5 the oxygen signal at zero cali...

Page 2: ...Net14 01 For sensor service remove the black POS seal tip 1 It is normal to see many small bubbles in the electrolyte reservoir This does not indicate that this caused a problem while the sensor was actually in use Remove the PEEK membrane ring 2 3 and membrane 4 Fig 2 Wash off the electrolyte with distilled water For cleaning of the anode and cathode the sensor head is best removed from the Orobo...

Page 3: ...ig 3 Hold the sensor in a vertical position and polish the cathode for one minute in a figure eight motion Fig 3 Wash the aluminum oxide powder carefully off the sensor with distilled water and wash the cleaning tools You may further clean the gold cathode with ultrasonic treatment at low power for up to 30 seconds while immersing the tip of the sensor head in distilled water In extreme and rare c...

Page 4: ...rystals Fig 4 As electrolyte use KCl solution 1 mol dm 3 74 56 g potassium chloride per litre in distilled water Add distilled water to the electrolyte powder Fig 1 up to the 10 ml mark Alternatively dissolve 1 49 g KCl in distilled water with a total volume of 20 ml Store at room temperature or 4 C in a closed bottle To prevent the formation of gas bubbles in the electrolyte heat the electrolyte ...

Page 5: ...2k is switched on Such stabilization may require several hours For this purpose the O2k may be left on overnight 4 Electrical cable connection Connect the POS cable to the O2k Main Unit avoiding bending and torsions of the cable Insert the male plug of the POS cable into the female connector next to the control light of the stirrer The red dot on the male plug has to face accurately upwards when i...

Page 6: ...t or drying the sensor body at 60 C for a few days the zero current should be reduced In addition the zero current of the bare cable without sensor head connected is tested for any leak currents If the latter test excludes any sources of leak currents other than the POS and POS service is not successful the sensor head must be replaced A new sensor head can be screwed onto the sensor connector if ...

Page 7: ...ights of the Oxygraph 2k left chamber between the MAINS and STIRRER lights right chamber between the STIRRER and COMM lights The ridge on the male plug has to face accurately downwards when inserting the plug before rotating the connector screw and fixing it finger tight O2k Manual MiPNet19 01A Oxygraph 2k start high resolution respirometry MiPNet14 01 Electrostatic discharge ESD damage and protec...

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