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MiPNet19.01 

B: OroboPOS service

 

20

 

 

 

OROBOROS INSTRUMENTS

 

 

OROBOROS Oxygraph-2k

 

Figure  2

.  Removal  of  a  used 

membrane from the POS. 

2

 

2. Grab the PEEK 

 

membrane ring 

 

on the sensor 

 

head

 

3

 

4

 

3. Pull the PEEK 

 

membrane ring from 

 

the sensor head

 

4. Remove and 

 

discharge the old 

 

membrane

 

Remove an old 

 

membrane from 

 

the POS

 

Membrane 

 

mounting tool

 

POS on POS 

 

connector

 

1. Remove 

 

POS seal tip

 

1

 

1.

 

Accessories 

 

Accessories  for  sensor  service  are  provided  in  the  OroboPOS-Service  Kit 

(Fig. 1).  In addition, you need distilled water and 25% ammonia solution 
(fresh). Store the OroboPOS in the dark. 

 

 

2.

 

Cleaning 

 

  Prevent  damage  by  electrostatic 

discharge  (ESD)  when  handling  the  POS 

connectors  or  cable  connections  to  the 
O2k (

MiPNet14.01

).  

 

 

For sensor service, remove the black 

POS seal tip (1). It is normal to see many 
small bubbles in the electrolyte reservoir. 
This  does  not  indicate  that  this  caused  a 
problem  while  the  sensor  was  actually  in 

use.  Remove  the  PEEK  membrane  ring 
(2,3)  and  membrane  (4;  Fig.  2).  Wash 

off the electrolyte with distilled water.  

 

 

For  cleaning  of  the  anode  and 

cathode,  the  sensor  head  is  best 
removed from the OroboPOS-Connector 

 

Fig. 1.

 

       

OroboPOS-Service Kit

   -    

Oxygraph-2k  

 

26300-01  OroboPOS-Electrolyte powder, KCl 

26200-01  OroboPOS-Membranes, FEP 25 µm; 40/Pck. 
26520-01  OroboPOS-Polishing Powder for cathode cleaning 

26510-01  OroboPOS-Polishing Cloth for cathode cleaning 
26400-01  OroboPOS-Mounting Tool for membrane application 

26800-01  Pipette\Plastic\1 ml ungraded for electrolyte 

26600-01  O2-Zero Powder, dithionite (Na

2

S

2

O

4

26550-01  Pen-Contact Oil for stable low contact resistance 

 

 

1

 

2

 

3

 

4

 

5

 

6

 

7

 

1

 

2

 

3

 

4

 

5

 

6

 

7

 

Summary of Contents for Oxygraph-2k

Page 1: ...s of operation Performance of the OROBOROS Oxygraph 2k according to instrumental specifications is obtained only with oxygen sensors which are maintained in a good functional state POS service is required if 1 a new sensor is prepared 2 the raw signal at air calibration declines 3 signal noise is high 4 the time response is prolonged time constant 10 s and biphasic 5 the oxygen signal at zero cali...

Page 2: ...Net14 01 For sensor service remove the black POS seal tip 1 It is normal to see many small bubbles in the electrolyte reservoir This does not indicate that this caused a problem while the sensor was actually in use Remove the PEEK membrane ring 2 3 and membrane 4 Fig 2 Wash off the electrolyte with distilled water For cleaning of the anode and cathode the sensor head is best removed from the Orobo...

Page 3: ...ig 3 Hold the sensor in a vertical position and polish the cathode for one minute in a figure eight motion Fig 3 Wash the aluminum oxide powder carefully off the sensor with distilled water and wash the cleaning tools You may further clean the gold cathode with ultrasonic treatment at low power for up to 30 seconds while immersing the tip of the sensor head in distilled water In extreme and rare c...

Page 4: ...rystals Fig 4 As electrolyte use KCl solution 1 mol dm 3 74 56 g potassium chloride per litre in distilled water Add distilled water to the electrolyte powder Fig 1 up to the 10 ml mark Alternatively dissolve 1 49 g KCl in distilled water with a total volume of 20 ml Store at room temperature or 4 C in a closed bottle To prevent the formation of gas bubbles in the electrolyte heat the electrolyte ...

Page 5: ...2k is switched on Such stabilization may require several hours For this purpose the O2k may be left on overnight 4 Electrical cable connection Connect the POS cable to the O2k Main Unit avoiding bending and torsions of the cable Insert the male plug of the POS cable into the female connector next to the control light of the stirrer The red dot on the male plug has to face accurately upwards when i...

Page 6: ...t or drying the sensor body at 60 C for a few days the zero current should be reduced In addition the zero current of the bare cable without sensor head connected is tested for any leak currents If the latter test excludes any sources of leak currents other than the POS and POS service is not successful the sensor head must be replaced A new sensor head can be screwed onto the sensor connector if ...

Page 7: ...ights of the Oxygraph 2k left chamber between the MAINS and STIRRER lights right chamber between the STIRRER and COMM lights The ridge on the male plug has to face accurately downwards when inserting the plug before rotating the connector screw and fixing it finger tight O2k Manual MiPNet19 01A Oxygraph 2k start high resolution respirometry MiPNet14 01 Electrostatic discharge ESD damage and protec...

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