www.oroboros.at
Service of the
polarographic
oxygen sensor
OroboPOS
Gnaiger E
OROBOROS INSTRUMENTS Corp
high-resolution respirometry
Schöpfstr 18, A-6020 Innsbruck, Austria
1. Accessories ............................................. 20
2. Cleaning ................................................. 20
3. Membrane mounting ................................ 22
4. Electrical cable connection ........................ 23
5. Storage of the POS .................................. 23
6. Replacement of the POS head ................... 24
7. References .............................................. 24
Summary:
Service of the polarographic oxygen sensors (
OroboPOS,
POS
) is the basis for signal stability, low noise and high time resolution.
Sensor service may not be required for several months of operation.
Performance of the OROBOROS Oxygraph-2k according to instrumental
specifications is obtained only with oxygen sensors which are maintained
in a good functional state.
POS service is required if (1) a new
sensor is prepared, (2) the raw
signal at air calibration declines, (3)
signal noise is high, (4) the time
response
is
prolonged
(time
constant >10 s) and biphasic, (5)
the oxygen signal at zero calibration
does not decline rapidly to a low
level of zero current (0% to max. 5%). For each sensor, the frequency of
POS service can be optimized on the basis of a long-term record of
calibration values as a quality control (O2k-Calibration-List.xls).
Mitochondrial Physiology Network 19.01(B01): 19-24 (2014)
2014 OROBOROS
B:
www.bioblast.at/index.php/MiPNet19.01B_POS-Service
Version B01: 2014-03-06
Oxygraph-2k Manual
B
