23
A12.0910
(1) Rotate the turntable, to turn the fluorescence filter B
or G into the light path. (If there is no fluorescence
filter B or G, use another fluorescence filter.) (See Fig.
40)
(2) Rotate the objective nosepiece, to turn the 10X
objective into the light path.
(3) Put the fluorescence flashboard block
①
to
position “O”, to open the light path.
(4) Focus the slide on the stage, and adjust it to be
clear.
(5) Pull the aperture diaphragm pole
②
to the outmost,
to open the aperture diaphragm to the smallest.
(6) Take off one eyepiece, replace it with the CT
(Centering Telescope), and insert it into the observation
tube. Adjust the CT to find the image of aperture
diaphragm in the view field.
(7) Adjust the two aperture diaphragm centering screws
③
on the side of illuminator by a inner hexagon
spanner, to move the image to the center of view field.
(See Fig. 40)
(8) Open the aperture diaphragm gradually, if the image
is inscribed with the view field, then it means the
aperture diaphragm is rightly centered.
(9) In actual fluorescence observation, push the
aperture diaphragm pole
②
, to open the aperture
diaphragm to the largest.
★
The aperture diaphragm is centered when leaving
factory, so the user does not have to readjust it.
★
If the high-brightness excitation light is used, the
fluorescence of sample will decrease, then firstly use
the ND filter to reduce the brightness of the
excitation light. If there is no ND filter, then narrow
the aperture diaphragm to reach the same function.
Fig.40
Fig.41
