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3) Use the fine focus knob to obtain a sharp image.
4) To get a good focused image, you may need to combine the focus knob adjustment
and interpupillary distance adjustment, along with eyepiece diopter adjustment
stated in
3.3
and
3.4
.
5) You may now switch to another magnification objective.
Tips:
To prevent your specimen slide from making contact
with an objective, raise the stage to its highest position
without contacting the 100X objective, then tighten the
focus stop lever (
Fig. 8
).
Give the stage a tiny extra
moving space to ensure the objective can be focused
every time.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in
Fig. 9 (a)
.
2) Place a drop of immersion oil on the slide cover as shown in
Fig. 9 (b)
.
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the lens cleaning paper to clean the 100X
objective lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and
the specimen.
Caution (important):
When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
When you apply the immersion oil with the 100X objective, do not let the
immersion oil to contact with the dry objective lenses (especially the 40X). If the
immersion oil is on the dry objectives lens, please use the lens cleaning paper to
clean the objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective
lens first.
Fig.8
Fig.9
Focus Stop Lever
(b)
(a)
