5
X -t
SlantLine-t
FreeLine-t
Y -t
Y-Z-t
X-Y-t
ZoomIn-t
X-Z-t
X-Y-t
X-Y-Z-t
ZoomIn-Z-t
Patch clamp
BX61WI fixed stage upright mictranslation stage
Image acquisition
ROI designation
Intensity versus time
measurement
Calcium sparks in isolated cardiac myocyte
Dr. Sandor Gyorke
Texas Technical University
X -t
X -t
Time Course
Using different scanning modes to chart time-lapse changes efficiently.
High-speed (4 frames/sec) image
acquisition
For the high speed observation of the
sample, Fluoview is capable of scanning 4
frames per second in a fast scanning mode
at an image size of 512X512. By limiting the
image size, the frame rate will be even
faster. This scanning mode is suitable for
living cell observation.
Versatile line scanning modes have
many uses
The wide variety of the line scanning modes
(linear/slant/free-line) enables flexible
analysis of rapid time-lapse experiments.
Superior slice patching system
In combination with the unique fixed stage &
nosepiece focusing BX61WI microscope,
the FV300 provides a highly effective
system for slice patching. This unique set-
up has a small footprint for increased room
in a space-limited cage. The remote control
microscope options minimize the danger of
accidentally touching the delicate
experimental settings.Olympus also offers
ideal non-cover glass long working distance
water immersion objectives and an optional
XY translation stage that moves the entire
confocal microscope system while the
sample and other experimental hardware
remains in a fixed position.
Highly precise time-lapse analysis
Fluoview’s wide dynamic range of 12-bit or
4096 grey levels provides enough sensitivity
to detect even the slightest changes in
intensity. The user can designate multiple
regions of interest (ROI) by using drawing
tools. The fluorescence intensity or the ratio
can be analyzed with the intuitive GUI driven
program.
Calcium wave in Xenopus oocyte, Calcium Green staining, fluorescence
pseudo-colored fluorescence image after injection of inositol 3-trisphospate
Japan Science and Technology Corporation, Exploratory Research for Advanced Technology,
Mikoshiba cell control project, Prof. Aya Muto
Calcium wave in isolated cardiac myocyte
Dr. Sandor Gyorke
Texas Technical University
Long working distance, non-cover
glass water immersion objective
Immersion-type LUMPLFL objectives
The 40X water immersion objective in this series has a
3.3mm working distance and an extremely fine tip which
is suitable for micromanipulation using a fixed stage
upright microscope. It has a large N.A. (0.8) and is also
ideal for confocal observations. When using the BX61WI
fixed stage & nosepiece focusing upright microscope
with water immersion objectives, confocal imaging can
be used to monitor time-lapse fluorescence changes in
thick specimens such as brain slices.
