background image

Preset the conditions for image
acquisition and loading

Storage of Acquisition Settings enables
immediate, one-touch recall of all the
relevant experimental settings and
conditions. Adding new conditions or
altering existing ones is quick and easy.

AOTF: flexible control of the laser
intensity to meet the specific
demands (optional)

The laser exposure will be limited within
the scanning area by default, minimizing
unnecessary bleaching of the specimen.
Option includes:
•Any laser intensity for any excitation area
("Region of Excitation" mode)
•Multiple laser applications
•AOTF controller that provides easy link
with external equipment

ZoomIn scanning

Zoom scanning can be
conducted over any
designated rectangular area.
Since only the region of the

targeted, zoomed-in area is acquired,
scan time and laser exposure of the
specimen is minimized.

Point scanning

The ultimate in fast scanning, 
the point scan enables
accurate quantitation of
intensity changes during rapid
physiological events.

Line scanning

A single line may be scanned,
oriented at any angle in the XY
plane. This fast scanning
option permits accurate

quantitation of physiological events such
as Calcium waves or sparks.

Free line scanning

Intensity changes may be
measured over a given period
of time along the length of a
freely drawn line, such as the

trace of an axon or along a cellular
junction.

Clip scanning

By cropping the image, selected areas
can be cut out of complex image stacks.

3

Flexible setting of scanning size,
zoom, movement and rotation

The observation field and scanning area
are both displayed graphically. Settings
can be confirmed while scrolling through
the zoom ratios. The “pan” button lets the
operator move the image acquisition area
at will, and rotation scanning of images is
also possible.

Simplified toolbar

A newly designed toolbar with various
dedicated buttons has greatly improved
ease of use. The user can execute a
succession of selected processes with
one-click operation for each.

Ultimate ease of operation and monitor display.

Software Graphical User Interface

Dye selection display

When a fluorescence dye is chosen, the
laser and light path settings are selected
automatically, with each of the selected
fluorescence dyes displayed graphically
on the monitor.

Innovative scanning method for
improved performance

ZoomIn

Spot

SlantLine

FreeLine

20%

50%

B

Versatile display options

Exchange between condensed and full
image display modes can be performed
with a single touch. Individual panel
layouts can be changed at will, and the
panel in use can be placed in any desired
position.

Summary of Contents for FLUOVIEW FV300

Page 1: ...CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW...

Page 2: ...stage upright microscope platforms the FV300 offers excellent versatility as well as top class laser scanning performance Highest image quality 12 bit 2048x2048 pixel resolution with economical cost E...

Page 3: ...r introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret 0 Emission beam split...

Page 4: ...ine scanning Intensity changes may be measured over a given period of time along the length of a freely drawn line such as the trace of an axon or along a cellular junction Clip scanning By cropping t...

Page 5: ...well Once optimized steps q r can be performed easily Scanning unit set up monitor display Human colon crypt Nuclei Blue TO PRO 3 Actin Green Alexa 488 APC gene product Red Alexa 568 Christine Anderso...

Page 6: ...er glass long working distance water immersion objectives and an optional XY translation stage that moves the entire confocal microscope system while the sample and other experimental hardware remains...

Page 7: ...low chart optional time course software TIEMPO Hardware and software support to optimize the environment for FRET FRET Input output of external trigger signal The optional time course software gives c...

Page 8: ...by describing the individual steps or phases within the experiment Users can specify detailed conditions and parameters for each step This function enables users to construct complex experimental prot...

Page 9: ...h point such as XYZ coordinates the Z axis acquisition range and the detector sensitivity 2 Up to 5 X 5 adjacent fields of view registered automatically Since adjacent fields of view are registered au...

Page 10: ...on driving mechanism that enables 0 01 m step control within the BX61 BX61WI and IX81 motorized microscopes high quality continuous cross sectional images can be acquired The 3D function also provides...

Page 11: ...hysioview software Axon PC side Working conditions Clampex Ver 8 0 or later Physiolink software FV300 side Colocalization image white Colocalization By using this function to analyze multi color speci...

Page 12: ...g pseudo colors Mituyo Kinoshita Pr Kentaro Arikawa Laboratory of Neuroethology Graduate School of Integrated Science Yokohama City University Purkinje cell in the rat cerebellum FITC vesicular GABA t...

Page 13: ...ish embryo Extended focus image of 10 mx28 slice Pr Yasuhiro Kamei Pr Shunsuke Yuba Institute for Molecular and Cellular Biology Osaka University GFP labeling of Drosophila adult brain with staining o...

Page 14: ...BA625IF BA660IF BA585 640 440 LD 405 LD 458 Multi Argon 488 Multi Argon 515 Multi Argon 543 Green HeNe 568 Krypton Argon 633 Red HeNe 488 Argon SDM515 SDM505 SDM570 RDM630 SDM630 SDM560 SDM600 ECFP E...

Page 15: ...lication Real time image calculation Kalman filtering peak integration software Hardware control Laser scanning unit microscope Each image display Single channel side by side merge cropping tiling ser...

Page 16: ...Multi Ar HeNe Red Green lasers Installation stand is not included in the unit Please consult your Olympus dealer for additional laser combinations External transmitted light detector system Fluorescen...

Reviews: