2.6
Scan Controls
2.6.1
Scan Mode, Figure 6.1
1. Unidirectional - Standard scan mode for routine imaging. Each line is scanned from left to right,
the laser beam is blanked while the scanner jumps back to the left side to scan the next line.
2. Bi-Directional – the specimen is scanned in both directions, odd lines are recorded from left to
right, even lines from right to left. This allows for extremely fast scanning of brief, rapid events.
There is a 30 sec. limit for bi-directional scanning.
2.6.2
Dwell Time, Figure 6.2
The Dwell Time is the length of time allotted for the focused laser spot to scan across each sample point
in the scan box. A longer dwell time increases the length of time spent moving over each point to
deliver more photons exciting the fluorescent label to generate signal. However, a longer dwell time
will increase photobleaching. The time required to scan a field is determined by the product of the
number of times each line is scanned, number of lines in the field, number of sample points per line and
the dwell time. Zoom does not affect scan time.
The numbers below the dwell time slider provide the effect of dwell time on image collection, for
example - P:4.0
µ
s L:3.144 ms F:1.644 s S:4.932 s
Figure 6. The Scan Mode and Scan Size settings of the Acquisition Setting Window.
6.5 Box Size
6.4 Image Aspect Ratio
6.6 Auto HV
6.3 Scan Regions
6.2 Dwell Time
6.1 Scan Modes
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011
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