Olympus BXFM Series, Instructions Manual

Page: 18 / 36

15
2 Setting the DIC Slider
(Fig. 18)
1. Loosen the mounting knob @ on the front of the DIC revolving nosepiece,
insert the DIC slider ² so that the surface with indication faces up, and
clamp by tightening the mounting knob.
2. With the U-DICR interference slider, set the slide lever ³ according to the
objective in use.
Fig. 18
@
²
³
|
Lever ³ position Applicable Objectives
Pushed in
UIS2 MPLFLN/MPLFLN-BD series
UIS UMPlanFl/UMPlanFl-BD series
MPlanApo20X, 100X
MPlanApo100XBD
Pulled out
UIS2 LMPLFLN/LMPLFLN-BD series
UIS LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series
3. With the U-DICRH or U-DICRHC slider that does not have the slide lever,
the applicable objectives are as follows.
DIC Slider Applicable Objectives
U-DICRH
MPLFLN/MPLFLN-BD series
UMPlanFl/UMPlanFl-BD series
MPlanFl-BD series
MPlanApo20X, 100X
U-DICRHC
LMPLFLN/LMPLFLN-BD series
LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series
UIS2
UIS
UIS2
UIS
3 Observation Method
(Fig. 18)
Place the specimen on the stage plate and adjust the focus by using the
coarse/fine adjustment knobs.
U-DICR U-DICRHC
1. Adjust the background contrast by turning the prism movement knob |
on the DIC slider as described below. (Fig. 18)
2. When the prism movement knob on the DIC slider is turned, the interfer-
ence color in the background varies continuously from the gray sensitive
color to magenta sensitive color (from -100 to 600 nm). Set the interfer-
ence color which can provide best contrast with respect to the specimen.
· Selecting a gray background color enables 3D-looking observation with
high contrast thanks to the highest sensibility of the gray sensitive color.
· Selecting a magenta sensitive color allows even small phase variation to
be observed as a change in color.