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LM

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o

2.

    

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one

 

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and

 

t

MW

 

Microscop

uorescence

 

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Click

 

the

 

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on

 

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off

 

by

 

p

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of

 

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tion

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the

 

lower

 

bar

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ropriate

 

fluor

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sure

 

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the

r

 

pushed

 

in)

 

a

c

y

 

sition

 

he

 

remote

 

co

utter

 

button

c

 

rescence

 

mirr

ods

 

buttons

 

e

 

light

 

path

 

b

and

 

XM10

 

cam

b

ontroller

 

box

 

t

c

 

on

 

the

 

rem

ror

 

cube

 

by

 

p

bars

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in

 

mera

 

is

 

select

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use

 

mercu

mote

 

box.

   

ressing

 

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(excep

correct

 

posit

ted.

  

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ry

 

arc

 

lamp

 

li

or

 

buttons

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pt

 

BF

 

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DIC

tion

 

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m p u s

 

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The

 

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X 5 3

 

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box

 

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mirror

 

wil

mid

 

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extend

f

 

 

G u i d e

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e

 

light

 

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position

 

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4

 

Summary of Contents for BX53

Page 1: ...epiec turning the no controls the b e condenser le d moves the s DOWN button e movement c s on the LED s X53 Digit t end time select USER ntrol unitc t orescence im ce camera o osepiece turr brightness of ens stage up or d n brings it dow can be selecte screen Note f tal Micro and enter th the Prior rem maging or both reth by hand transmission own to focus wn quickly F ed High H M DON T USE t b c ...

Page 2: ...tage election of im irror cube for t shutter ic image acqu he program f orescence ima x objective by w will pop up n the messag ns on the Obs e with Prior z maging mode r different co uisition or your imag aging for fluor y pressing an Turn the obj ge window It servation Me stage contro O l y i e BF or Flu lors of fluore ing needs i e rescently labe objective jective nosep t is critical to m thods...

Page 3: ...uisition era is ON and is pushed in and obtain p ith Spot 30 Click the but ctory click Sn king the right XM10 mono tic exposure de list You can choos selected from and the lowe proper exposu region and a tton again to nap t mouse butto ochrome cam to obtain bet se a lower res m the camera er bar11 is p ure and white utomatic wh stop live view button and s on over the im O l y mera for fluor tter i...

Page 4: ...emote co utter buttonc rescence mirr ods buttons e light path b and XM10 cam b ontroller box t c on the rem ror cube by p barsf are in mera is select to use mercu mote box ressing Mirro excep correct posit ted O l y ry arc lamp li or buttonsD pt BF and DIC tion the uppe e m p u s B X ight for fluore in the contro C The select er bar at the X 5 3 U s e r escence The oller box or cl ed mirror wil mi...

Page 5: ... tiff files Se bit image Us ultiple channe images for ea th View Scale tamp comma image as JPE e acquisition w eck the Auto en and TxRe o show curren ot 30 is use ditions are set the Automat n to read the ed click the ulting multich er as soon as t at without an elect Image M se File Save a els Or use Fil ach channel e bar comma and These an EG JFIF jpg with an exam matic Proces button d red chann...

Page 6: ... uplicate if it i dow tab ast of the disp ram or the Di oose the cha ick the color c st the histogr is necessary t from play can be ad splay Enhanc nnels to be v channels you ram and the b O l y to make any c m the main wi djusted cement appli visible eye sy want to show brightness co m p u s B X changes in th indow tabs in es to all the v mbol indi w and click o ontrast of the X 5 3 U s e r e ima...

Page 7: ...l im e image type f From the Ed peration will g Use Edit Cop cale bar in pix sion Selector channel imag d click OK mage you just from 16 bit g it menu use generate a co py Display to xel unit losin r Use Image ges Then use O l y t made as abo grayscale to 8 Edit Select A mposite imag o Clipboard an g some dime Separate Ch e Image Com m p u s B X ove there ar bit color All then Edit ge with the sa ...

Page 8: ...image me from a z st ame view and enu range of fram w starting pos se a new end ake sure all th n and select E ame will be ge Objective s acquired at el To set the ntroller or by the stage to fo buttonc wh er region close Image acquis end a step siz this step size accordingly es You can co can be manip ack by clickin d selecting Ex mes within the sition within t position and e channels ar Extract ...

Page 9: ...ed y ack frames int aximum Proje you can displ y cross hairlin ptions d x y slice pos to a single 2D ection Over Z ay a cross sec e to the desir sition D image by sel on the naviga ctional z dime red position such as O l y lecting Proces ator bar men ension view o Extended Slic m p u s B X ss Projection u of an x and y ce X 5 3 U s e r ns Maximum plane You ca you can visu G u i d e 9 m Z command a...

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