4.3 Olink Focus Workflow with the 24.192 chip
NOTE:
Prepare your primers and samples as described in the separate instructions for the specific panel.
4.3.1 Prepare the 24.192 chip
Before pipetting assays and samples into the 24.192 chip, prepare the chip with control line fluid.
IMPORTANT:
When injecting control line fluid:
• Follow the best practices for handling chips and control line fluid, see
“3.3.1 Chip and Control Line Fluid Handling”
• Only use an Olink Control Line Fluid for a 24.192 chip syringe. Control line fluid syringes are prefilled according
to a specific chip type.
1. Remove the control line fluid syringe from the packaging and the chip from the box and foil envelope.
IMPORTANT:
Do not evacuate air from the syringe before injecting control line fluid (Step
).
Optional step to ensure proper chip function:
2. Actuate the top check valve:
a. Place the chip on a flat surface.
b. Use the syringe with the shipping cap to actuate the check valve in the top accumulator with gentle pressure.
Ensure that the poppet can move freely up and down (
).
Cap
Check valve
Notched
corner
Top accumulator
Bottom accumulator
DO NOT USE!
Figure 11. Actuating the check valve in the top accumulator on the 24.192 chip
3. Hold the syringe firmly in 1 hand with the tip facing up and away from the chip, pull back on the plunger slightly to
create negative pressure, and remove the shipping cap with the other hand. Be careful when removing the syringe cap
of the control line fluid to prevent drips.
4. Holding the chip at a 45° angle, insert the syringe tip into the top accumulator (
IMPORTANT:
• Avoid bending the syringe tip. Be careful when removing the syringe cap to prevent drips.
• Avoid getting control line fluid on the exterior of the chip or in the inlets because this makes the chip unusable.
If this occurs, use a new chip.
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