45
Figure 64. T Sensor attached on the bottom of the Petri dish (a -b).
Tip
►
Use the same dish that you use in regular practice.
•
Fill the sample holder with water or oil to the level used during standard operation. Make sure the tip
of the T Sensor is immersed in the liquid. Cover the sample holder with the lid or leave it open,
according to the protocol followed during standard operation.
•
Close the incubating chamber with its lid.
•
Make sure that the microscope objective heater is equipped with the OBJ-COLLAR. Make sure that the
Objective Heater touches the sample in both oil immersion and water dipping imaging.
•
Be sure that the reading of T Sensor is stable and then press
Manual
button (see pointer 3 in Figure
62 b) and insert the offset value clicking on
+
or
-
on the Offset tab (see pointer 1 in Figure 65 b) to
adjust the Objective Heater offset value and press on
OK
to save (see pointer 2 in Figure 65 b). The
offset is defined as the temperature difference between the set point and the reading of T Sensor.
a
b
1
2
Figure 65. Settings
–
Calibrations
–
Obj. Heater - Manual (a -b).
•
To verify if the
Manual
procedure ends correctly, wait until the reading of T Sensor is stable and check
if it is equal to the set point value. If this does not happen, insert a new manual offset as the difference
between the set point temperature and the reading of T Sensor. Repeat the procedure until the
reading of T Sensor becomes equal to the set point.
4.
By pressing
Calibrate
button (see pointer 4 in Figure 62 b) UNO-CONTROLLER will perform a calibration
routine for your specific environment.