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H301-MIZAR-TILT

User Manual

REV 02

www.oko-lab.com

Pa

ge

1

H301-MIZAR-TILT

Compatible with

Compatible with the following Okolab Controllers

•

Mizar Tilt system

•

UNO-T

•

UNO-T-H-PREMIXED

•

UNO-T-H-CO2

•

H301-T-UNIT-BL-PLUS

•

Bold Line Gas controllers

IST1748_REV02

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2
3
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Summary of Contents for H301-MIZAR-TILT

Page 1: ...al REV 02 www oko lab com Page 1 H301 MIZAR TILT Compatible with Compatible with the following Okolab Controllers Mizar Tilt system UNO T UNO T H PREMIXED UNO T H CO2 H301 T UNIT BL PLUS Bold Line Gas controllers IST1748_REV02 ...

Page 2: ...5 4 1 Available Sample Holders 5 4 2 Available Lid 5 5 Insertion of the Sample Feedback Temperature Sensor 6 6 Working with OKO MH Sample Holder 7 7 Working with 1 x3 chamber slide 9 8 Working with 35mm petri dish 11 9 Working with MIZAR CUBE GL 13 10 Connection of the Gas Supply 13 11 Working with perfusion 14 12 Connection of the Chamber with Z stage 14 13 Cleaning 15 ...

Page 3: ...sor Light Trap to avoid any reflected light Magnetic Clamps to snap lock the chamber slide Chamber base with embedded temperature sensor Chamber Bracket to fix the chamber to the stage Distancebetween focal plane and upper side of the glass lid Chamber top view Chamber Lid Light Trap Magnetic Clamp Chamber Base Chamber Bracket Figure 1 H301 MIZAR TILT Components and Dimensions ...

Page 4: ... guide arm LIGHT TRAP GS Light trap for 1 x3 slides chamber slides LIGHT TRAP MCH LIGHT TRAP GS Figure 2 Available Light Trap 3 Available Magnetic Clamp H301 MIZAR TILT can accept standard glass slide with thickness between 1 50 up to 2 mm and also glass slide with thickness between 2 1 to 4 mm The following magnetic clamps are available for H301 MIZAR TILT MAG CLAMP 1 5 2 Magnetic clamp for slide...

Page 5: ... cannot find the sample holder you are looking for We are constantly adding new inserts to the list MIZAR 1x35 Holder for 35mm petri dish MIZAR 1x22by40 Holder for 22x40 mm coverslip MIZAR 1X35 MIZAR 1x22by40 Figure 4 MIZAR 1X35 and MIZAR 1x22by40 Sample holder 4 2 Available Accessories MIZAR CUBE GL Stainless Steel Top Lid for 12 50x12 50 Mizar Imaging Cube It helps to minimize evaporation Figure...

Page 6: ...e medium temperature and realize a self calibration of the chamber fix the Temperature Sensor tip with adhesive tape on the bottom of the medium volume of the sample holder For more information about the self calibration please refer to the User Manual of the Okolab Controller in use Sensor Tip AdhesiveTape Opening for Temperature Sensor 1 Frontal view 2 Temperature Sensor Placement Figure 6 Inser...

Page 7: ...nd listed below 1 Insert the MIZAR 1x22by40 into the Chamber Base see image 1 in Figure 8 2 Place the magnetic clamps over the insert see image 2 in Figure 8 then push down the head of the clamps sim ultaneously and move it in the direction shown in the image 3 of Figure 8 3 Insert the Light Trap into the Chamber Base as shown in image 4 in Figure 8 4 Close the Chamber Base with the Lid see image ...

Page 8: ...H301 MIZAR TILT User Manual REV 02 www oko lab com Page 8 1 2 3 4 5 6 Figure 8 Working with MIZAR 1x22by40 Holder ...

Page 9: ...he steps shown in Figure 10 and listed below 1 Insert the 1 x3 chamber slide into the Chamber Base see image 1 in Figure 10 2 Place the magnetic clamps over the 1 x3 chamber slide see image 2 in Figure 10 then push down the head of the clamps simultaneously and move it in the direction shown in the image 3 of Figure 10 3 Insert the Light Trap into the Chamber Base as shown in image 4 in Figure 10 ...

Page 10: ...H301 MIZAR TILT User Manual REV 02 www oko lab com Page 10 1 2 3 4 5 6 Figure 10 Working with 1 x 3 chamber slide ...

Page 11: ...see image 1 in Figure 12 2 Place the magnetic clamps over the 1 MIZAR 1x35 see image 2 in Figure 12 then push down the head of the clamps simultaneously and move it in the direction shown in the image 3 of Figure 12 3 Turn the flat springs see image 4 in Figure 12 and insert the petri dish see image 5 in Figure 12 into the sample holder 4 Lock the petri dish by using the flat springs see image 6 i...

Page 12: ...H301 MIZAR TILT User Manual REV 02 www oko lab com Page 12 1 2 3 4 5 6 7 8 Figure 12 Working with 35mm petri dish ...

Page 13: ...o insert the Sample Feedback Temperature Sensor see 2 in Figure 13 Temperature Sensor dedicated opening 1 Insertion of MIZAR CUBE GL 2 Temperature Sensor Figure 13 Working with MIZAR CUBE GL 10 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 MIZAR TILT Silicon tubing con nects to a gas input brass opening located on rear side of th...

Page 14: ...hows the location of the perfusion holes Openings for Perfusion 1 Frontal view 2 3D view Figure 15 Perfusion 12 Connection of the Chamber with Z stage To fix the H301 MIZAR TILT on the stage follow the steps listed below and shown in Figure 16 1 Fix the bracket to the SLR 300 Piezoconcept Stage with four M2 5x6 screws see Image 1 of Figure 16 2 Fix the base of the chamber with two M3x6 screws on t...

Page 15: ...he chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 MIZAR TILT_User Manual Rev ...

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