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H301-ALPHA3-SMALL 

User Manual 

   

REV 02

 

 

 www.oko-lab.com 

  

 

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4

 

2.

 

Insertion of the Sample Feedback Temperature Sensor 

Insert  the  Sample  Feedback Temperature  Sensor  through the  dedicated  opening  located  in  the  H301-ALPHA3-SMALL 

(see Figure 2). 

To read the temperature  of the  culture medium and realize a self-calibration of the chamber, fix the Temperature 

Sensor tip with adhesive tape on the sample holder inside the H301-ALPHA3-SMALL. For more information about the 

self-calibration, please refer to the User Manual of the Okolab Controller in use. 

 

Figure 2. Insertion of the temperature sensor inside the chamber. 

3.

 

Connection of the Gas Supply 

A single silicon tubing carries output gas from the Okolab Gas Controller to H301-ALPHA3-SMALL. Silicon tubing con-

nects to a gas input - brass opening - located on a corner of the H301-ALPHA3-SMALL (see Figure 3). Connect by gently 

pushing silicon tubing onto brass opening. 

Gas Input

 

Figure 3.Connection with gas supply. 

Summary of Contents for H301-ALPHA3-SMALL

Page 1: ...ser Manual REV 02 www oko lab com Page 1 H301 ALPHA3 SMALL Compatible with the following microscope Compatible with the following Okolab Controllers PhaseView Alpha3 Light Sheet microscope Bold Line U...

Page 2: ...www oko lab com Page 2 Index 1 Components and dimensions 3 2 Insertion of the Sample Feedback Temperature Sensor 4 3 Connection of the Gas Supply 4 4 Working with perfusion 5 5 Installing the H301 ALP...

Page 3: ...ons H301 ALPHA3 SMALL includes the following components Objective Collar compatible with water immersion objective 21 mm diameter Sample Arm Cover Chamber Lid Chamber Base Chamber Base Objective Colla...

Page 4: ...ape on the sample holder inside the H301 ALPHA3 SMALL For more information about the self calibration please refer to the User Manual of the Okolab Controller in use Figure 2 Insertion of the temperat...

Page 5: ...with perfusion 1 Use the supplied Stainless Steel Perfusion Tubes Attach a Silicon tubes O D 1 6mm I D 0 8mm not supplied to the Stainless Steel Perfusion Tubes see image 1 in Figure 4 2 Insert the S...

Page 6: ...Note Please pay attention to the sample holder glass 4 Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided hexagonal key see image 2 in Figure 5 5 Place...

Page 7: ...H301 ALPHA3 SMALL User Manual REV 02 www oko lab com Page 7 5 4 2 1 3 7 6 Figure 5 Connection of the Chamber with Stage H301 ALPHA3 SMALL_User Manual Rev 02_Published in September 2020...

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