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H201-NIKON-TI-S-ER

User Manual

August 2019

www.oko-lab.com

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2

Index

Components and dimensions................................................................................................... 3

Available Sample Holders ....................................................................................................... 3

Available Lids ..................................................................................................................... 4

Sliding lid .......................................................................................................................... 5

Koehler Lid ........................................................................................................................ 6

Injection Lid ...................................................................................................................... 6

Laser Interlock Lid ............................................................................................................... 7

Insertion of the Sample Feedback Temperature Sensor .................................................................. 8

Insertion of Sample Holder into Chamber ................................................................................... 8

Working with 35 or 60 mm Petri Dish

Spacing Rings and Magnetic Locks ........................................... 9

Working with 1x3’’ and 1x2’’ chamber slides

- magnetic locks ........................................................11

Working with MW Plates - Magnetic locks and Chamber riser ..........................................................12

Connection of the Gas Supply ................................................................................................13

Working with Perfusion ........................................................................................................13

Connection of the Chamber with adapter ..................................................................................16

Connection of the Chamber with XY stage .................................................................................17

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Summary of Contents for H201-NIKON-TI-S-ER

Page 1: ...ith the following XY stages Compatible with the following Okolab gas modules Nikon Ti S E and Ti S ER IST450_rev01 CO2 UNIT BL CO2 UNIT BL CP2 CO2 O2 UNIT BL CP2 O2 UNIT BL 1 20 CO2 O2 UNIT BL 0 10 1 18 CO2 O2 UNIT BL 0 20 1 95 CO2 O2 UNIT BL 0 10 0 1 2GF MIXER 3GF MIXER HYPOXIA 3GF MIXER HYPEROXIA ...

Page 2: ...tion of the Sample Feedback Temperature Sensor 8 5 Insertion of Sample Holder into Chamber 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 9 7 Working with 1x3 and 1x2 chamber slides magnetic locks 11 8 Working with MW Plates Magnetic locks and Chamber riser 12 9 Connection of the Gas Supply 13 10 Working with Perfusion 13 11 Connection of the Chamber with adapter 16 12 Co...

Page 3: ... the chamber riser Chamber top view Sliding Lid Chamber Riser Chamber Base Sample Holder Order Separately Adapter Figure 1 H201 NIKON TI S ER Components and Dimensions 2 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1x35 M 1 35mm Petri...

Page 4: ...etri dish H201 MW HOLDER Multiwell plates holder Figure 2 Available sample holders 3 Available Lids The following glass Lids are available for H201 NIKON TI S ER H201 SLIDING LID For easy sample loading and pipetting Included in code H201 NIKON TI S ER H201 KOEHLER LID Reduces chamber height to 21 mm and allows imaging under Koehler illumination H201 INJECTION LID Glass lid with two small openings...

Page 5: ...VED when using any other Lid The Sliding Lid is fixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Sliding Lid Distance between focal plane and upper side of the glass lid without the chamber riser Distance between focal pla...

Page 6: ... and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber riser Chamber top view H201 KOHLER LID Figure 5 H201 NIKON TI S ER H201 KOEHLER LID 3 3 Injection Lid The Injection Lid is a glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample NOTE Compatible with s...

Page 7: ...ety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser Distance between focal plane and upper side of the glass lid without th...

Page 8: ... Temperature Sensor dedicated opening Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Fig...

Page 9: ...0 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 11 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively 1 3 2 Figure 11 1 Insertion of the ring 2 insertion of the 35 mm...

Page 10: ...lco 35x10 Grainer Petri35x10 Ibidi μ Dish 35 mm low MatTek P35G X14 X Corning 35x10 mm Figure 12 Ring selection scheme for 35 mm dish Corning 60x15 mm BD Falcon 60x10 Ibidi μ Dish 50 mm low Grainer Petri 60x15 MatTek P50G X30 X Willco 60x15 Figure 13 Ring selection scheme for 60 mm dish ...

Page 11: ...cks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 14 Magnetic lock for 1 x 3 chamber slide 1 2 3 Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Page 12: ...es holder must be ordered separately NOT INCLUDED Product code H201 MW HOLDER Figure 16 Magnetic locks for MW plates To be ordered separately Chamber Riser increases chamber s height and is REQUIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 17 shows how to install and fasten the chamber riser in place Screws location is indicated by letter A A Chamber Riser A Chamber Base Fi...

Page 13: ...ut Figure 18 Connection with gas supply 10 Working with Perfusion The Chamber Riser included with H201 NIKON TI S ER features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter To lock the Chamber Riser into the base use a 1 5mm Allen key to secure the 4 GUTB2 2 5 6 screws as shown in Figure 19 Figure 19 How to lock the Chamber Riser Small screws plug the perfu...

Page 14: ...H201 NIKON TI S ER User Manual August 2019 www oko lab com Page 14 1 Frontal view 2 3D view Perfusion dedicated opening Figure 20 Perfusion Figure 21 How to access the perfusion holes ...

Page 15: ...with 19x3 mm windows on the two short sides to allow insertion of microfluidic tubings Windows are magnetically closed Compatible with most of H201 and H301 Okolab chambers Thickness of this chamber riser is 8mm 2mm more than default chamber riser Requires Koehler lid and single ac commodation specimen holders Figure 22 MICROFLUIDIC C RISER ...

Page 16: ...the captive screws in the proper housings 2 Place the chamber on the adapter as shown in Figure 23 Image 3 3 Use a 1 5mm metric Allen Wrench to tighten two of the four captive screws in place A in Image 4 while keeping the sliding lid closed See Figure 23 Image 4 4 Tighten the remaining two captive screws A in Image 5 while keeping the sliding lid open 5 Image 6 of Figure 23 shows the chamber fixe...

Page 17: ...rub screws to align correctly the adapter on the stage Grub screws housings are indicated with letter A in Image 2 of Figure 24 3 Follow the arrows shown in Image 3 of Figure 24 for placing flat socket head cap screws use the provided Allen wrench for tightening the flat socket head cap screws and fastening the adapter on the stage Screws housings are indicated with letter B in Image 4 of Figure 2...

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