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H201-LG-DLS

User Manual

September 2016

www.oko-lab.com

Pa

ge

2

Index

Components and dimensions................................................................................................... 3

Sample Holders ................................................................................................................... 4

Available Sample Holders ....................................................................................................... 4

Insertion of the Sample Feedback Temperature Sensor .................................................................. 5

Removing and Inserting the Lid ............................................................................................... 6

Insertion of Sample Holder into Chamber ................................................................................... 7

Working with 35 or 60 mm Petri Dishes ....................................................................................10

Connection of the Gas Supply ................................................................................................12

Working with Perfusion ........................................................................................................12

Connection of the Chamber with Super Z-Galvo stage ...................................................................12

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Summary of Contents for H201-LG-DLS

Page 1: ...ember 2016 www oko lab com Page 1 H201 LG DLS Compatible with the following XY stages Compatible with the following Okolab Controllers LEICA super Z Galvo stage SP5 SP8 H201 T BL PLUS UNO STAGE TOP INCUBATOR H401 T DUAL Vers 01 16 ...

Page 2: ...Available Sample Holders 4 3 Insertion of the Sample Feedback Temperature Sensor 5 4 Removing and Inserting the Lid 6 5 Insertion of Sample Holder into Chamber 7 6 Working with 35 or 60 mm Petri Dishes 10 7 Connection of the Gas Supply 12 8 Working with Perfusion 12 9 Connection of the Chamber with Super Z Galvo stage 12 ...

Page 3: ...y it owns 12 holes for the insertion of perfusion tubing up to 2 0 mm Objective adapter it fits HC APO L 10x 0 3 W or Leica HC FLUOTAR L 25x 0 95 W Sample Holder it allows the Petri to be hold in the chamber Lid it features a magnetic system allowing to lift and release the Lid surrounding the objective together with it Total chamber weight 130g Figure 1 H201 LG DLS Components and Dimensions ...

Page 4: ...le Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1x35 DLS 1 35mm Petri dish 1x60 DLS 1 60mm Petri dish Figure 2 Available sample holders ...

Page 5: ... Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 LG DLS main body see 1 Frontal View 2 3D View Figure 3 Frontal and 3D views 1 Frontal View 2 3D View Figure 3 Insertion of the temperature sensor inside the chamber ...

Page 6: ...b Microscope Pillar Cover follow the instructions as shown in Figure 4 Figure 4 Changing the Microscope Pillar Cover 5 Removing and Inserting the Lid The H201 LG DLS is equipped with a magnetic Lid which directly holds into the provided microscope pillar cover If you want to access the specimen or you want to remove the sample holder you should remove the Lid first to re move it follow the steps s...

Page 7: ...the Lid 6 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by captive screws embedded the holder Screw the sample holder using a 1 5 mm Allen key don t care about the orientation since the sample holder is symmetric as illustrated in Figure 6 ...

Page 8: ...If you want to change the sample holder follow the steps shown in the images of Figure 7 and listed below 1 Remove the chamber body by lifting it from the workspace as shown in Figure 7 Image 1 and 2 2 Unscrew the sample holder captive screws using a 1 5 mm Allen key 3 Lift and replace the sample holder as shown in Figure 7 Image 4 ...

Page 9: ...H201 LG DLS User Manual September 2016 www oko lab com Page 9 Figure 7 Introduction of the Specimen Holder inside the Chamber Base ...

Page 10: ... steps below 1 Slightly loose the sample holder screws denoted as A in Image 1 of Figure 8 and Figure 9 using a flat head screw driver in order to have the locks released 2 Place the Petri in its holder as in Image 2 1 of Figure 8 and Figure 9 3 Block the sample holder screws denoted as B in Image 1 of Figure 8 and Figure 9 using a flat head screwdriver in order to have Petri locked in position Fi...

Page 11: ... com Page 11 Figure 9 Plate adapter for 60 mm Petri dish holder Note On the sample holders for petri dishes there are two water reservoirs see Image 4 of Figure 8 and Figure 9 Fill them with water to ensure a high humidity percentage inside the chamber ...

Page 12: ...DLS features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2 0 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M2 5x6 Remove small screws as nec essary before introducing perfusion tubing Figure 11 shows the location of perfusion holes 1 Frontal View 2 3D View Figure 11 Perfusion 10 Connection of the Chamber with Super Z Galvo st...

Page 13: ...H201 LG DLS User Manual September 2016 www oko lab com Page 13 Figure 12 How to fix the H201 LG DLS on the Super Z Galvo stage ...

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